Development of of nanospace spectral microscope

纳米空间光谱显微镜的研制

• 批准号: 13558087
• 负责人: ITOH Shigeru
• 金额: $ 9.22万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-13558087/
• 关键词: Confocal microscope; Cyanobacteria; Heterocyst; Chlorosome; Excitation energy transfer; 1 molecular measurement; Coral; Photosynthesis; ナノ空間; 植物多様性; 顕微鏡; 顕微分光; 蛍光スペクトル; 単分子分光; イメージング; 共焦点

A new confocal laser microscope was developped to measure spectral features of absorption and fluorescence of biological materials and single molecules. The microscope developed has wavelength accuracy of 1nm, spacial resolution of 0.2 micrometer, respectively, and is equipped with a liquid He cryostat to enable observation at 350-4K. It can be ued for the spectral analysis of micro-organs inside living cells as well as of single molecules. We measured spectral differentiation of cyanobacterial vegetative cells and heterocysts, each chloroplast inside plant cells, chlorosome single molecules isolated from anaerobic photosynthetic bacteria. It was shown that each cell, molecules are fluctuating due to thermal agitation, biological hystory, and environmental differentions. The direct measurement of fluctuaion on each member of group gives new insight for the gene-ecpression, molecular structure etc.

研制了一种新的激光共聚焦显微镜，用于测量生物材料和单分子的吸收光谱和荧光光谱特征。研制的显微镜波长精度为1 nm，空间分辨率为0.2微米，并配备了液体氦低温恒温器，可在350-4K下进行观察。它既可用于活细胞内微小器官的光谱分析，也可用于单分子的光谱分析。我们测量了蓝藻营养细胞和异形胞、植物细胞内的每个叶绿体、从厌氧光合细菌中分离的叶绿体单分子的光谱分化。结果表明，每个细胞、分子都是由于热搅拌、生物细胞和环境差异而波动的。直接测量基团每个成员的起伏，为基因表达、分子结构等提供了新的见解。

项目成果

Gembos, Z., V'arkongi, Z., Hago M., Iwaki, M., Kov'acs, L., Masumoto, K., Itho, S.: "Regirement of phosphatidilglycerol for the function of electron acceptor plastoqninone QB in the PSII vacation canton"Biochemistry. 41. 3796-3802 (2002)

Gembos, Z.、Varkongi, Z.、Hago M.、Iwaki, M.、Kovacs, L.、Masumoto, K.、Itho, S.：“磷脂酰甘油对电子受体质体醌 QB 功能的调节

Itoh, S., Fukushima, Y., Itoh, K.Maeda, M, Mino, H., Kumazaki, S.: "Photosystem I and II reaction centers in a new type oxygenic photosythesis of Acharoehloris marina"I Journal of Photo science. 9巻2号. 70-73 (2002)

Itoh, S.、Fukushima, Y.、Itoh, K.Maeda, M、Mino, H.、Kumazaki, S.：“Acharoehlolis 码头新型含氧光合作用中的光系统 I 和 II 反应中心”I 摄影科学杂志第 9 卷第 2 期。70-73 (2002)

Ono, T., Rompel, A., Mino, H.and Chiba, N.: "Ca^<2+> function in photosynthetic oxygen evolution studied by alkali metal cation substitution"Biophysical Journal. 81. 1831-1840 (2001)

Ono, T.、Rompel, A.、Mino, H. 和 Chiba, N.：“通过碱金属阳离子取代研究光合产氧中的 Ca^2 功能”生物物理学杂志。

Itoh, S., et al.: "Photosystem I and II Reaction Centers in a New Type Oxygenic Photosynthesis of Acaryochioris marina Based on Chlorophyll d : Studies of Delayed Fluorescence and Triplet State ESR"J Photoscience. 9. 70-73 (2002)

Itoh, S., et al.：“基于叶绿素 d 的 Acaryochoriris marina 新型产氧光合作用中的光系统 I 和 II 反应中心：延迟荧光和三重态 ESR 的研究”J Photoscience。

Itoh.S., et al.: "Photosystem I and II Reaction centers in a New Type Oxygenic Photosynthesis of Acaryochloris marina Based on Chlorophyll d : Studies of Delayed Fluorescence and Triplet State ESR"J Photoscience. 9. 70-73 (2002)

Itoh.S. 等人：“基于叶绿素 d 的 Acaryocholis marina 新型产氧光合作用中的光系统 I 和 II 反应中心：延迟荧光和三重态 ESR 的研究”J Photoscience。