Mechanism of Postmortem Tenderization of Meat Estimated by Monoclonal Antibody
单克隆抗体评估肉类宰后嫩化机制
基本信息
- 批准号:02453135
- 负责人:
- 金额:$ 4.03万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for General Scientific Research (B)
- 财政年份:1990
- 资助国家:日本
- 起止时间:1990 至 1992
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
The mechanism of thaw rigor and tenderization of meat was studied by using monoclonal antibody against myofibrillar protein. Following results were obtained.1. Production of monoclonal antibody against native connection or titn was successful. The identity of connectin and titin in the native state was immunologically suggested on the basis of immuno-blotting and ELISA methods.2. The factors affecting the conversion of alpha-connectin to beta-connectin induced by pressurization of muscle were investigated over a pressure range from 100 to 400 MPa by using SDS-PAGE and immuno-blot analysis. When muscles were exposed to high pressure, the conversion of alpha-connectin to beta-connectin was markedly accelerated by pressurization at 200 MPa, but further increase of the con-version was not observed in the muscle pressurized at 300 and 400 MPa. The ability of calcium-activated protease ( CAF ) to hydrolyze connectin from alpha to beta was gradually reduced with increasing intensity of pressure. The obtained results indicates that CAF is responsible for the pressure-induced conversion of alpha- to beta-connectin.3. The immunoelectron microscopic studies using monoclonal antibody against connectin or titin revealed that the antibody-binding stripes between connectin and titin were different. One distinct antibody-binding stripe was recognized at the both side of the H-zone in the center of the A-band in the fiber bundles treated with antibody against connectin, while two stripes were recognized in each half of the A-band in the fiber bundles treated with antibody against titn. The gradual degradation of the stripe recognized at the both side of the H-zone in the fiber bundles treated with antibody against connectin was observed in the muscle stored at 2゚C for 7 days. This is one of the evidence that the filament structure consisting of connectin is gradually degraded during postmortem conditioning.
用抗肌原纤维蛋白的单抗研究了肉的解冻僵硬和嫩化机理。取得了以下研究结果:1.成功地制备了抗天然连接或TITN的单抗。通过免疫印迹和酶联免疫吸附试验,从免疫学角度提出了天然状态下连接蛋白和肌动蛋白的同源性。用SDS-PAGE和免疫印迹分析研究了肌肉加压诱导α-连接素向β-连接素转化的影响因素。当肌肉暴露于高压时,200 Mpa加压可显著加速α-连接素向β-连接素的转化,但300和400 Mpa加压时,转换率没有进一步增加。钙激活的蛋白水解酶(CAF)将连接素从α向β转化的能力随着压力的增加而逐渐降低。研究结果表明,CaF参与了压力诱导的α-连接向β-连接的转化。用抗连接蛋白和肌动蛋白的单抗进行免疫电子显微镜观察发现,连接蛋白和肌动蛋白之间的抗体结合条带不同。在用抗连接素抗体处理的纤维束中,在A带中心的H带两侧识别出一条明显的抗体结合条纹,而在用抗TITN抗体处理的纤维束中,在A带的每一半处识别出两条明显的抗体结合条纹。在2゚C保存7天的肌肉中,用抗连接素抗体处理的纤维束中H区两侧识别的条纹逐渐降解。这是由连接素组成的细丝结构在死后条件处理过程中逐渐退化的证据之一。
项目成果
期刊论文数量(22)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
鈴木 敦士・金 健・池内 義秀: "超高圧処理によるαーコネクチンからβーコネクチンへの変換" 第84回日本畜産学会大会講演要旨. 242-242 (1991)
Atsushi Suzuki、Ken Kane、Yoshihide Ikeuchi:“通过超高压处理将 α-连接蛋白转化为 β-连接蛋白”日本动物科学学会第 84 届年会摘要 242-242 (1991)。
- DOI:
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- 影响因子:0
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- 通讯作者:
Kim Ken: "Pressure-Induced Conversion of α-Connectin to β-Connectin" Meat Scince. 32. 237-243 (1992)
Kim Ken:“压力诱导的 α-Connectin 向 β-Connectin 的转化”《肉类科学》32. 237-243 (1992)。
- DOI:
- 发表时间:
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- 影响因子:0
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Suzuki Atsushi: "Acceleration of Meat Conditeoning by High Pressure Treatment" Abstract of First European Seminor on High Pres-sure & Biotechbology,a joint meeting with the Fifth Simpo.on High Pressure & Food Science. 21 (1992)
铃木厚:“通过高压处理加速肉类调理”第一届欧洲高压研讨会摘要
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
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岡本 明: "高圧処理が筋小胞体に及ぼす影響" 第87回日本畜産学会大会講演要旨集. (1993)
Akira Okamoto:“高压治疗对肌浆网的影响”日本动物科学学会第 87 届年会摘要(1993 年)。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Suzuki Atsushi: "Acceleration of Meat Conditioning by High Pressure Treatment" High Pressure and Biotechnology, Eds. C.Balny, R.Hayashi, K.Heremans and P.Masson, Colloque INSERM / John Libbey Eurotext Ltd.Vol.224. 219-227 (1992)
铃木敦:“通过高压处理加速肉类调理”高压和生物技术,编辑。
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