Genetic control of experimental autoimune uveitis in the mouse

小鼠实验性自身免疫性葡萄膜炎的遗传控制

• 批准号: 02454401
• 负责人: MOCHIZUKI Manabu
• 金额: $ 4.22万
• 依托单位: Kurume University School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1990
• 资助国家: 日本
• 起止时间: 1990 至 1991
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-02454401/
• 关键词: mouse; retinal soluble antigen; autoimmune uveitis; B10 congenic mouse; Hー2ハプロタイプ; マウス; 免疫遺伝学

A model of experimental autoimmune uveitis(EAU)in the mouse was established by an enhanced immunization(2-4 times injection at an interval of 2-4 weeks)with S-antigen or interphotoreceptor retinoid binding protein(IRBP)using Krebsielia lipopolysaccharide(K03 LPS)as an adjuvant. The incidence of EAU was tested in several strains of B10. BR, B10. A, B10. MBR, B10. A(4R)were found to be high responders to S-antigen-induced EAU as well as to IRBP-induced EAU. On the other hand, B10. A(5R)was non-responder to S-antigen-induced EAU and low-responder to IRBP-induced EAU. These data thus indicate that the susceptibilty to EAU in the mouse was restricted by the immune response genes(H-2), particulady by 1-A^k. The susceptibility to EAU in AKR/J was very low, although H-2 haplotype ws identical to B10. BR. Therefore, it is suggested that non-MHC genes also plays some minor role in EAU.

以克雷伯氏菌脂多糖（K03 LPS）为佐剂，用S抗原或光感受器间视黄醇结合蛋白（IRBP）强化免疫（注射2~4次，间隔2~4周），建立小鼠实验性自身免疫性葡萄膜炎（EAU）模型。在几种 B10 菌株中测试了 EAU 的发生率。 BR，B10。 A、B10。 MBR，B10。发现 A(4R) 对 S 抗原诱导的 EAU 以及 IRBP 诱导的 EAU 具有高反应性。另一方面，B10。 A(5R)对S抗原诱导的EAU无应答，对IRBP诱导的EAU低应答。这些数据因此表明小鼠对EAU的敏感性受到免疫应答基因(H-2)，特别是1-A^k的限制。尽管 H-2 单倍型与 B10 相同，但 AKR/J 对 EAU 的敏感性非常低。 BR。因此，表明非 MHC 基因在 EAU 中也发挥着一些次要作用。

项目成果

K Iwase,M Minami,I Nakashima,Y Fujii,N Kato and M Mochizuki: "Ocular Immunology Today" Elsevier, 4 (1990)

K Iwase、M Minami、I Nakashima、Y Fujii、N Kato 和 M Mochizuki：“今日眼部免疫学”Elsevier，4 (1990)

K Iwase,Y Fujii,I Nakashima,N Kato,Y Fujino,H Kawashima and M Mochizuki: "A new method for induction of experimental autoimmune uveoretinitis (EAU)in mice" Current Eye Research. 9. 207-216 (1990)

K Iwase、Y Fujii、I Nakashima、N Kato、Y Fujino、H Kawashima 和 M Mochizuki：“诱导小鼠实验性自身免疫性葡萄膜视网膜炎 (EAU) 的新方法”当前眼科研究。

Ko Iwase, Yasuaki Fujii, Izumi Nakashima, Nobuo Kato, Yujirou Fujino, Hidetoshi Kawashima and Manabu mochizuki: "A new method for induction of experimental autoimmune uveoretinitis (EAU) in mice" Current Eye Research. 9. 207-216 (1990)

Ko Iwase、Yasuaki Fujii、Izumi Nakashima、Nobuo Kato、Yujirou Fujino、Hidetoshi Kawashima 和 Manabu mochizuki：“诱导小鼠实验性自身免疫性葡萄膜视网膜炎 (EAU) 的新方法”当前眼科研究。

Ko Iwase, Mutushiko Minami, Izumi Nakashima, Yasuaki Fujii, Nobuo Kata and Manabu Mochizuki: "Experimental autoimmune uveitis (EAU) in the mouse : H-2 restriction of EAU induction" Ocular Immunology Today Eds. M. Usui, S. Ohno and K. Aoki. Elsevier, Amste

Ko Iwase、Mutushiko Minami、Izumi Nakashima、Yasuaki Fujii、Nobuo Kata 和 Manabu Mochizuki：“小鼠实验性自身免疫性葡萄膜炎 (EAU)：EAU 诱导的 H-2 限制”今日眼部免疫学编辑。

K Iwase,Y Fujii,I Nakashima,N Kato,Y Fujino,H Kawashima and M Mochizuki: "A new mothod for induction of experimental autoimmune uveoretinitis(EAU)in mice" Current Eye Research. 9. 207-216 (1990)

K Iwase、Y Fujii、I Nakashima、N Kato、Y Fujino、H Kawashima 和 M Mochizuki：“诱导小鼠实验性自身免疫性葡萄膜视网膜炎 (EAU) 的新方法”当前眼科研究。