Analysis of gene and protein expression on infiltrating lymphocytes

利用从眼炎性疾病、葡萄膜炎等不同临床实体患者眼内浸润细胞建立的T细胞克隆，研究了眼内浸润淋巴细胞基因和蛋白的表达谱。比较结节病患者与Vogt-Koyanagi-Harada病患者眼部浸润细胞的差异。只有四个基因在结节病中比在Vogt-Koyanagi-Harada病中强3倍。另一方面，在Vogt-Koyanagi-Harada病中，72个基因的表达比结节病强3倍。结节病中T细胞受体α链的表达量是Vogt-Koyanagi-Harada病的3.4倍，而Vogt-Koyanagi-Harada病中T细胞受体δ链和可溶性δ链的表达量分别是结节病的6.2倍和5.4倍。两种疾病的眼部浸润淋巴细胞亚群完全不同：结节病以αβ淋巴细胞为主，而Vogt-Koyanagi-Harada病以γδ淋巴细胞为主。一般认为，γδ淋巴细胞占机体T淋巴细胞的1-5%，主要存在于皮肤和粘膜上皮。它们在先天免疫中起着重要作用。γδ淋巴细胞在眼内浸润，以往未见报道。本研究首次报道了γδ淋巴细胞浸润在Vogt-Koyanagi-Harada病患者的眼睛中。Vogt-Koyanagi-Harada病是一种黑色素细胞特异性的自身免疫性疾病。存在于眼睛和皮肤中的黑素细胞是包括γδ淋巴细胞在内的T淋巴细胞自身免疫反应的目标。Vogt-Koyanagi-Harada病眼部γδ淋巴细胞可能与皮肤γδ淋巴细胞有关。提示Vogt-Koyanagi-Harada病的免疫致病机制可能是γδ淋巴细胞对黑素细胞的交叉反应和免疫识别。少

Expression profile of gene and protein on infiltrating lymphocytes in the eye was investigated using T cell clones established from infiltrating cells in the eye of patients with various clinical entities of ocular inflammatory disorders, uveitis.Ocular infiltrating cells in patients with sarcoidosis and patients with Vogt-Koyanagi-Harada disease were compared each other. There were only four genes which were three times stronger in sarcoidosis than in Vogt-Koyanagi-Harada disease. On the other hand, in Vogt-Koyanagi-Harada disease, 72 genes were expressed three times stronger than in sarcoidosis. In sarcoidosis, T cell receptor αchain expression was 3.4 times higher than Vogt-Koyanagi-Harada disease, while in Vogt-Koyanagi-Harada disease T cell receptor δ chain and soluble δ chain were 6.2 times and 5.4 times higher than sarcoidosis, respectively. These data suggest that subset of infiltrating lymphocytes in the eye was completely different in the two diseases : ocular infiltrating ly … More mphocytes were αβ lymphocytes in sarcoidosis whereas γδ lymphocytes in Vogt-Koyanagi-Harada disease. It is generally understood that γδ lymphocytes consist of 1-5% of T lymphocytes in the body and present mainly in the skin and epithelium of the mucosa. They play an important role in the innate immunity. It has not reported previously that γδ lymphocytes infiltrate in the eye. This study is the first to report that γδ lymphocytes infiltrate in the eye of patients with Vogt-Koyanagi-Harada disease.Vogt-Koyanagi-Harada disease is an autoimmune disease specific to melanocytes. Melanocyte existing both in the eye and the skin are the targets of autoimmune reactions by T lymphocytes including γδ lymphocytes. The γδ lymphocytes found in the eye of Vogt-Koyanagi-Harada disease might be related to γδ lymphocytes in the skin. It is suggested that immunopathogenic mechanisms of Vogt-Koyanagi-Harada disease might be a cross reaction and immunological recognition by the γδ lymphocytes against melanocytes. Less