Clinical and basic research on the pathogenic mechanisms of HTLV-I uveitis

HTLV-I葡萄膜炎发病机制的临床及基础研究

• 批准号: 06454501
• 负责人: MOCHIZUKI Manabu
• 金额: $ 4.67万
• 依托单位: Kurume University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06454501/
• 关键词: HTLV-I; uveitis; T cell clone; viral load; cytokine; HTLV-I infected lymphocyte; HTLV-Iぶどう膜炎; RT-PCR; 定量的PCR; virus load

To investigate the pathogenic mechanisms of HTLV-I uveitis (HU), immunological and molecular biological studies were carried out using infiltrating cells in the eye and peripheral blood monocytes (PBM) of patients with HU.The proviral DNA of HTLV-I was detected in the ocular fluid of HU patients by polymerase chain reaction (PCR), and the gene expression of HTLV-I was detected from the ocular infiltrating cells at mRNA level by RT-PCR.In addition, the gene expression of cytokines (IL-1alpha, IL-2, IL-3, IL-4, IL-6, IL-8, IL-10, TNF-alpha, IFN-gamma and GM-CSF) was examined by RT-PCR and the mRNA of IL-6 was detected from ocular infiltrating cells. T cell clones (TCC) were established from ocular infiltrating cells and PBM of HU patients by limiting dilution methods. HTLV-I proviral DNA (+) TCC expressed HTLV-I associated proteins and viral particles were found in TCC from ocular fluid by electron microscopic examination. The TCC from ocular fluid and PBMC produced IL-1alpha, IL-6, IL-8, TNF-alpha, IFN-gamma and GM-CSF.These findings suggest that HTLV-I infected cells are presented in the eye of HU patients and the HTLV-I infected cells produce a variety of cytokines which modulate the intraocular inflammation.

为探讨HTLV-Ⅰ型葡萄膜炎（HU）的发病机制，采用免疫学和分子生物学方法对HU患者眼内浸润细胞和外周血单核细胞（PBM）进行了研究。RT-PCR检测眼浸润细胞中HTLV-I的mRNA表达，并检测细胞因子的基因表达RT-PCR检测IL-1 α、IL-2、IL-3、IL-4、IL-6、IL-8、IL-10、TNF-α、IFN-γ和GM-CSF，并检测IL-6 mRNA。采用有限稀释法从HU患者的眼浸润细胞和PBM中建立T细胞克隆（TCC）。电镜观察发现，TCC中存在HTLV-I前病毒DNA（+），TCC表达HTLV-I相关蛋白和病毒颗粒。结果表明，HU患者眼内存在HTLV-I感染细胞，并产生多种细胞因子，调节眼内炎症反应。

项目成果

Kazuhiro Masuoka: "Polyclonal use of T-cell receptor α for human T-cell lymphotropic virus type 1-infected T cells" Invest Ophthalmol Vis Sci. 36. 254-258 (1995)

Kazuhiro Masuoka：“T 细胞受体 α 在人类 T 细胞嗜淋巴细胞病毒 1 型感染的 T 细胞中的多克隆用途”Invest Ophasemol Vis Sci 36. 254-258 (1995)

Kazunari Yamaguchi: "Human T-lymphotropic virus type I uveitis" Leukemia. 8(Suppl). 88-89 (1994)

Kazunari Yamaguchi：“人类 T 淋巴细胞病毒 I 型葡萄膜炎”白血病。

Ayako Ono, Tomoyuki Miura, Shinji Araki, Kazunari Yamaguchi, Kiyoshi Takatsuki, Shigeo Mori, Masanori Hayami, Manabu Mochizuki, Toshiki Watanabe: "Subtype analysis of HTLV-1 in patients with HTLV-1 uveitis." Japanese Journal of Cancer Research. 85. 767-77

Ayako Ono、Tomoyuki Miura、Shinji Araki、Kazunari Yamaguchi、Kiyoshi Takatsuki、Shigeo Mori、Masanori Hayami、Manabu Mochizuki、Toshiki Watanabe：“HTLV-1 葡萄膜炎患者中 HTLV-1 的亚型分析。”

Kumiko Nakao, Norio Ohba, Hajime Inomata, Masahiro Yamamoto, Kenji Ohshima, Hideyuki Hayashi, Shinobu Akiya, Kenji Ibi, Manabu Mochizuki, Koichi Yoshimura, Eiko Ikeda, Kazuo Nakatsuka, Hiromi Ono, Shinji Oono, Eiichi Shimada, Ryoichi Okamura, Koichiro Ina

中尾久美子、大场纪夫、猪又一、山本雅宏、大岛贤二、林英幸、秋谷忍、伊比健二、望月学、吉村浩一、池田英子、中冢一夫、小野弘美、大野真司、岛田英一、冈村亮一、伊那晃一郎

Kazunari Yamaguchi: "Human Tlymphotropic virus type 1 uveitis after Graves' disease" Br.J.Ophthalmol.78. 163-166 (1994)

Kazunari Yamaguchi：“格雷夫斯病后人类嗜淋巴细胞病毒 1 型葡萄膜炎”Br.J.Ophasemol.78。