Analytical ultracentrifuge with absorption and interference detector

带吸收和干涉检测器的分析超速离心机

• 批准号: 430159603
• 金额: --
• 依托单位: Friedrich-Alexander-Universität Erlangen-Nürnberg
• 依托单位国家: 德国
• 项目类别: Major Research Instrumentation
• 财政年份: 2019
• 资助国家: 德国
• 起止时间: 2018-12-31 至 无数据
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/430159603?language=en
• 关键词: Analytical; ultracentrifuge; absorption; interference; detector

The disperse properties of particles, i.e. their size, shape, density, surface and optical properties, directly define the product properties in a variety of applications. This relationship, known as the property function, is of fundamental importance for academia and industry. A precise access to the disperse properties is essential to characterize and control product properties of particle systems in the sense of structure-property relationships.Analytical ultracentrifugation is a very powerful method for the investigation of complex and polydisperse particle systems in the liquid phase. The distribution of particles and macromolecules is detected as a function of time and radial position in a centrifugal field of up to 250,000 g. This data allows determination of the sedimentation and diffusion coefficient distributions. The hydrodynamic properties can in turn be correlated with the particle size, mass, shape and density. Interferometric measurements will be carried out with the device so that even non-scattering and non-absorbing analytes can be investigated with high temporal and radial resolution. This is important for the investigation of polydisperse samples, as the particle size-dependent sedimentation is compensated by the high resolution of the detector. For concentration studies, the small changes in the sedimentation boundaries are resolved and the influences of non-ideality can be investigated. In case the interference detector cannot be used, e.g. because the sample cannot be dialyzed or the influence of additives on the measurement signal cannot be determined, the absorption detector is applied. This detector can be operated simultaneously and allows for temporally and spatially resolved absorption and turbidity measurements. Thus, analytes are distinguished not only by their hydrodynamic properties but also by their spectral properties. For slowly sedimenting analytes, the detector can be used to measure several wavelengths in successive measurements, enabling global analyses. The absorption detector can be used for measurements in the deep UV and is therefore of great importance for polymers, proteins and quantum dots. Besides, it is possible to use both detectors for many material systems, which allows evaluation of experimental with global models.The proposed instrument will significantly expand the possibilities of multidimensional particle analysis at the applicant's institute. A large number of university, national and international collaboration partners will benefit from the device, as it will be integrated into numerous research initiatives.

颗粒的分散性能，即其尺寸、形状、密度、表面和光学性能，直接决定了产品在各种应用中的性能。这种关系被称为属性函数，对学术界和工业界都具有根本的重要性。精确地获得颗粒的分散性质是从结构-性质关系的角度来表征和控制颗粒体系的产品性质的关键，分析超离心法是研究液相中复杂和多分散颗粒体系的一种非常有效的方法。在高达250，000 g的离心场中，检测颗粒和大分子的分布随时间和径向位置的变化。该数据允许沉降和扩散系数分布的测定。流体动力学性质又可以与颗粒尺寸、质量、形状和密度相关。干涉测量将与设备进行，以便即使是非散射和非吸收的分析物可以调查高时间和径向分辨率。这对于多分散样品的研究非常重要，因为检测器的高分辨率可以补偿颗粒尺寸相关的沉降。对于浓度研究，可以解决沉降边界的微小变化，并研究非理想性的影响。如果不能使用干扰检测器，例如因为样品不能被透析或不能确定添加剂对测量信号的影响，则应用吸收检测器。该检测器可以同时操作，并允许时间和空间分辨的吸收和浊度测量。因此，分析物的区别不仅在于其流体动力学性质，而且在于其光谱性质。对于缓慢沉降的分析物，检测器可用于在连续测量中测量多个波长，从而实现全局分析。吸收探测器可用于深紫外测量，因此对聚合物、蛋白质和量子点非常重要。此外，这两种探测器都可以用于许多材料系统，从而可以对全球模型进行实验评估。拟议的仪器将大大扩展申请人研究所多维粒子分析的可能性。大量的大学，国家和国际合作伙伴将受益于该设备，因为它将被集成到许多研究计划中。