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Paternity Discrimination by DNA Polymorphism and Reproductive Strategy of Higher Non-human Primates

DNA多态性的亲子鉴定和高等非人灵长类动物的繁殖策略

基本信息

批准号：
03454003
负责人：
TAKENAKA Osamu
金额：
$ 3.33万
依托单位：
KYOTO UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1991
资助国家：
日本
起止时间：
1991 至 1992
项目状态：
已结题

项目摘要

Paternity in groups of various higher non-human primate species cannot be established reliably on behavioral grounds because of their multi-male group structure and promiscuous mating patterns. The present work aimed the establishment of paternity discrimination by analyses DNA polymorphism and application to wild and captive chimpanzee groups and Japanese monkey groups. Bood collection from wild chimpanzee is entirely impossible. The polymerase chain reaction (PCR) technique can amplify specific hypervariable DNA segments. This technique is applicable to partially degraded DNA extracted from body hair, faces, and wedges.DNA segments containing GT/AC dinucleotide repeats in the chimpanzee and Japanese macaque genome were screened. The sets of primer for PCR were designed from the nucleotide sequences flanking the dinucleotide repeats. Six and three sets of primer were successfully applicable to chimpanzees and Japanese macaques, respectively. Paternity of 40 offspring were examined in a chimpanzee breeding colony in captivity. The results suggest 1) a positive (through not conclusive) correlation between the male rank and the number of offspring, and 2) the choice of mating partners by the female rather than by the male. The analyses in the wild chimpanzees at Bossou, south-eastern Guinea revealed an identified non-resident adult male from an adjacent group was suspected of being the father of a offspring born in 1986-1987, although in three offspring out of four, the resident adult male likely their father.

在各种高等非人类灵长类物种的群体中，由于它们的多雄性群体结构和混杂的交配模式，父权制不能在行为基础上可靠地建立起来。本工作旨在通过分析DNA多态性建立亲权鉴别，并应用于野生和圈养黑猩猩群体和日本猴群体。从野生黑猩猩身上收集血液是完全不可能的。聚合酶链反应（PCR）技术可以扩增特异性高变DNA片段。该技术适用于从体毛、面部和楔形物中提取的部分降解的DNA。筛选了黑猩猩和日本猕猴基因组中含有GT/AC二核苷酸重复的DNA片段。从二核苷酸重复序列侧翼的核苷酸序列设计用于PCR的引物组。6套和3套引物分别成功应用于黑猩猩和日本猕猴。在一个圈养的黑猩猩繁殖群中对40只后代进行了亲子鉴定。结果表明：（1）雄性等级和后代数量之间存在正相关（尽管不是决定性的）;（2）交配伴侣的选择由雌性而不是雄性决定。对几内亚东南部Bossou野生黑猩猩的分析显示，一个来自邻近群体的非居民成年雄性被怀疑是1986-1987年出生的后代的父亲，尽管在四个后代中有三个，居民成年雄性可能是他们的父亲。