权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Multiple alpha-globin genes of crab-eating monkey : Resistance to malarial infection

食蟹猴的多个α珠蛋白基因：抵抗疟疾感染

基本信息

批准号：
05041089
负责人：
TAKENAKA Osamu
金额：
$ 1.15万
依托单位：
Primate Research Institute, Kyoto University
依托单位国家：
日本
项目类别：
Grant-in-Aid for international Scientific Research
财政年份：
1993
资助国家：
日本
起止时间：
1993 至无数据
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-05041089/
关键词：
Crab-eating monkey Macaque Gene alpha-globin gene Malaria-resistant Morphology Development Adaptation

项目摘要

Livinig organisms show a variety of adaptation to natural environment through evolution, that changed in nucleotides in their genes. For example, animals living in highland or underground have hemoglobins of high oxygen affinity. Regional disease is also an environmental factor for natural animals. We found that the crab-eating macaques of Malayan penninsula and Sumatra have multiplied alpha-globin genes in high frequency and those monkeys have high hematological values. The present study was undertaken to access the hypothesis that crab-eating monkeys can adapt themselves against malarial infection by gene duplication.We performed capture-and-release field works of crab-eating macaques in several localities in Thailand. Under anesthetization, morphological data and blood specimens were collected. Thick and thin blood smear was also prepared for microscopic observation of malarial infection. Hemato logical values were measured near from the field and buffy coat was brought back to Japan. The number of alpha-globin gene will be determined by Southern blot analysis.In the field work, the morphological data were also collected. By combining with the previous data, the crab-eating monkeys in Thailand have big body size and heavy body weight as compared with those of Malaysia, Indonesia and the Philippines. In Thailand, crab-eating monkeys of Songklah, southern part were the biggest followed by those of northeastern part. The smallest was Yala, again, southern part. This results contrasted with the clinal trait of genetic data.

生命体通过进化表现出对自然环境的各种适应，这些适应在其基因中的核苷酸中发生了变化。例如，生活在高原或地下的动物具有高氧亲和力的血红蛋白。区域性疾病也是自然动物的一个环境因素。我们发现，马来半岛和苏门答腊岛的食蟹猕猴有大量的α-珠蛋白基因，这些猴子有很高的血液学价值。本研究的目的是探讨食蟹猴通过基因复制来适应疟疾感染的假说，我们在泰国的几个地方对食蟹猴进行了捕获和释放的实地考察。在麻醉状态下，收集形态学数据和血液标本。还制备了厚血涂片和薄血涂片，用于显微镜观察疟疾感染。在田间附近测量血液学值，并将血沉棕黄层带回日本。通过Southern杂交分析确定α-珠蛋白基因的数目，并在田间采集形态学资料。结合以往资料，泰国食蟹猴体型较大，体重较马来西亚、印尼、菲律宾等地的食蟹猴重。在泰国，南部宋卡的食蟹猴最多，其次是东北部。最小的是亚拉，也是南部。这一结果与遗传数据的临床特征形成对照。