权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

STUDIES ON STRAIN-PRESERVATION AND FUNCTION-DEVELOPMENT OF AQUATIC PLANTS

水生植物菌种保存与功能开发研究

基本信息

批准号：
03454086
负责人：
SAGA Naotsune
金额：
$ 3.71万
依托单位：
TOKAI UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1991
资助国家：
日本
起止时间：
1991 至 1992
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-03454086/
关键词：
Macroalgae Microalgae Liquid Nitrogen Cryopreservation Strain-preservation Biological Function Aquatic Plants

项目摘要

Cryopreservation of the conchocelis of Porphyra yezoensis by the two-step cooling method was attempted. A solution composed of 10% DMSO and 0.5 M sorbitol in 50% seawater had the most favorable cryoprotective effect on the conchocelis. The appropriate cryopreservation procedure was as follows: DMSO was added gradually over a period of 15 min followed by an equilibration period of several minutes. Conchocelis cells were then cooled slowly to -40ﾟC prior to immersion in liquid nitrogen. After storage in liquid nitrogen, the conchocelis suspension was thawed quickly by agitation of the vial in a water bath at 40ﾟC, and cryoprotectants were washed off by gradual dilution with seawater. The survival was independent of the period of storage at least for 300 d. The survival evaluated by staining with neutral red was approx. 60%. The conchocelis cells stored in liquid nitrogen were able to form colonies, and retained the ability to form conchospores which grew to gametophytic thalli. This two- … More step cooling method is applicable to other macroalgae (e.g. Enteromorpha intestinalis, Macrocystis pyrifera).Hydrogen photoproduction capability in 39 strains of newly isolated marine and brackish nonsulfur phototrophic bacteria was surveyed. Among these, TU-PSB 105-1, which was isolated from a mud sample collected at brackish environment, exhibited highest hydrogen production capability (80 mulH_2・(mg prot)^<-1>・hr^<-1>). Using this strain TU-PSB 105-1, effects of nitrogen sources, substrates, pH and sodium chloride concentration to the hydrogen production capability were examined. Higher rates of hydrogen production were observed in glutamate-grown cells than in ammonia-grown cells. Organic acids such as fumarate, malate and succinate were more effective as a substrate for hydrogen production than sugars such as fructose and glucose. However, utilization of glucose was enhanced by preculturing the cells with glucose. Optimum hydrogen production was observed at low concentration of added sodium chloride (approx. 0.5%). At 0.5% of added sodium chloride, the rate of 160 mulH_2・(mg prot)^<-1>・hr^<-1> observed. Less

采用两步冷却法对紫菜螺壳进行了低温保存。10% DMSO和0.5 M山梨醇在50%的海水中组成的溶液对螺螺的冷冻保护效果最好。适当的冷冻保存程序如下：在15分钟内逐渐加入DMSO，然后经过几分钟的平衡期。然后将螺细胞缓慢冷却至-40℃，然后浸泡在液氮中。在液氮中保存后，在40°C的水浴中搅拌瓶快速解冻，并用海水逐渐稀释去除冷冻保护剂。至少300 d的存活期与存贮期无关。中性红染色评估的存活期约为。60%。保存在液氮中的螺壳细胞能够形成菌落，并保留形成螺孢子的能力，螺孢子发育为配子体体。这种两步以上的冷却方法也适用于其他大型藻类（如Enteromorpha肠肠、Macrocystis pyrifera）。对39株新分离的海洋和半咸淡水非硫光养细菌的产氢能力进行了研究。其中，从半苦环境下采集的泥浆样品中分离得到的TU-PSB 105-1产氢能力最高（80 mulH_2·（mg prot）^<-1>·hr^<-1>）。以该菌株TU-PSB 105-1为研究对象，考察了氮源、底物、pH和氯化钠浓度对其产氢能力的影响。谷氨酸培养的细胞比氨培养的细胞产氢率更高。富马酸、苹果酸和琥珀酸等有机酸作为产氢底物比果糖和葡萄糖等糖更有效。然而，葡萄糖预培养可以提高细胞对葡萄糖的利用。在添加低浓度氯化钠（约为2）时，产氢效果最佳。0.5%)。在添加0.5%氯化钠时，观察到160 mulH_2·（mg prot）^<-1>·hr^<-1>的速率。少