Distribution in Tissue and Biosynthesis of ABO Blood Group Substances Specific for Tissue.

在本研究中，首次将我们已经生产的确定鼠尾草ABO血型物质组织特异性表位的单克隆抗体应用于研究唾液腺中ABO血型物质的分布。用荧光或金胶体结合血型抗体作为第二抗体，在显微镜和电镜下观察，该抗体仅与唾液腺粘膜细胞发生反应，而与其他灵长类和动物的唾液腺粘膜细胞不发生反应。不幸的是，由于本实验所用的唾液腺采集自尸体，因此ABO血型物质或其前体在细胞内的分布并不明显。接下来，我们建立了针对肠化生的特异性单克隆抗体。结果表明，抗体识别的碳水化合物链仅在胃肠化生中表达，携带ABO和Lewis血型活性的碳水化合物链也存在于抗体定义的糖蛋白分子上。由于众所周知，肠化生组织中ABO和Lewis血型的活性低于正常组织，因此抗体识别的碳水化合物链可能是生物合成途径中对ABO和Lewis血型有活性的碳水化合物链的前体或前体样物质。此外，该抗体在亲和层析中可纯化约数百万kD的抗原糖蛋白。因此，我们考虑通过从大量胃粘膜中纯化抗原糖蛋白，并明确该抗体所定义的碳水化合物链结构，来清除组织中ABO血型物质的生物合成途径。

In this study, the monoclonal antibody defining tissue-specific epitope on the ABO blood group substances in salvia, which has been already produced by us, was first applied for investigation to the distribution of the ABO blood group substances in salival gland. In the microscopic and electron microscopic observation using fluorescence or gold colloid conjugated blood group antibody as a second antibody, the antibody reacted with only mucous cells in salival gland, but not react with the the those in salival gland of other primates and animals. Unfortunately, the intracellular distribution of the ABO blood group substances or its precursors ws not obvious, since salival gland used in this experiment was collected from cadavers.Next, we established a monoclonal antibody specific for intestinal metaplasia. It was made clear that the antibody recognized the carbohydrate chain expressing in only intestinal metaplasia of stomach, and that the carbohydrate chain carrying ABO and Lewis blood group activities also existed on the glycoprotein molecules defined by the antibody. Since it is well known that the tissue of intestinal metaplasia shows lower activity of ABO and Lewis blood groups than normal tissues, it seems that the carbohydrate chain recognized by the antibody is a precursor or a precursor-like material of the carbohydrate chain active to ABO and Lewis blood groups in the biosynthetic pathway. Furthermore, about millions kD antigenic glycoprotein could be purified in the affinity chromatography using the antibody. Therefore, we consider to clear biosynthetic pathway of the ABO blood group substances in the tissue by purifying of the antigenic glycoprotein from a large amount of gastric mucosa, and by making out obvious the structure of the carbohydrate chain defined by this antibody.