Expression of brain/embryo-type myosin heavy chain isoform in the healing process of damaged tissue

本文用组织化学和生物化学方法研究了脑/胚胎型肌球蛋白重链异构体（MIIB 2）在四氯化碳（CCl 4）诱导的大鼠肝炎愈合过程中的表达。在细胞增殖和成熟（分化和重建）之间瞬时观察到MIIB 2表达增加，表明MIIB 2在受损组织的分化或重建中起关键作用。因此，MIIB 2的表达似乎是愈合过程中细胞增殖终止的标志，用于损伤组织的修复和再生。在我们的下一步研究中，我们研究了大鼠比目鱼肌组织愈合过程中MIIB 2的表达，该组织通过插入18 G针或注射BaC 12溶液损伤。然而，我们没有发现肌肉组织愈合过程中MIIB 2表达的实质性增加。目前尚不清楚肝组织和肌肉组织愈合过程差异的原因，我们可以说我们几乎成功地完成了我们项目的目标。现在，我们将在法医实践中应用MIIB 2的表达用于损伤诊断。

Expression of brain/embryo-type myosin heavy chain isoform (MIIB2) in the healingprocess of rat hepatitis induced by administration of CCl4 was investigated histochemically and biochemically. Increasing expression of MIIB2 was transiently observed between cell proliferation and maturation (differentiation and reconstruction), suggesting that MIIB2 plays a crucial role in differentiation or reconstruction of damaged tissue. Therefore, it seems that MIIB2 expression is a marker for termination of cell proliferation for repair and regeneration of damaged tissue in the healing process.In the next step of our study, we investigated MIIB2 expression in the healing process of the rat soleus muscle tissue, which was damaged by insertion of an 18G needle or injection of BaC12 solution. However, we could not find substantial increase of MIIB2 expression in the healing process of muscle tissue. It is not clear the reasons responding to the difference between healing processes of the hepatic tissue and the muscle tissue at present.We can say that we have succeeded almost object of our project. Now, we are going to apply the expression of MIIB2 for diagnosis of injury in medico-legal practice.