Expression of brain/embryo-type myosin heavy chain isoform in the healing process of damaged tissue

脑/胚胎型肌球蛋白重链亚型在受损组织愈合过程中的表达

• 批准号: 09670448
• 负责人: TSUJI Tsutomu
• 金额: $ 1.92万
• 依托单位: Wakayama Medical College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09670448/
• 关键词: healing; brain; embryo-type myosin heavy chain isoform; cell proliferation; cell differentiation; experimental hepatitis

Expression of brain/embryo-type myosin heavy chain isoform (MIIB2) in the healingprocess of rat hepatitis induced by administration of CCl4 was investigated histochemically and biochemically. Increasing expression of MIIB2 was transiently observed between cell proliferation and maturation (differentiation and reconstruction), suggesting that MIIB2 plays a crucial role in differentiation or reconstruction of damaged tissue. Therefore, it seems that MIIB2 expression is a marker for termination of cell proliferation for repair and regeneration of damaged tissue in the healing process.In the next step of our study, we investigated MIIB2 expression in the healing process of the rat soleus muscle tissue, which was damaged by insertion of an 18G needle or injection of BaC12 solution. However, we could not find substantial increase of MIIB2 expression in the healing process of muscle tissue. It is not clear the reasons responding to the difference between healing processes of the hepatic tissue and the muscle tissue at present.We can say that we have succeeded almost object of our project. Now, we are going to apply the expression of MIIB2 for diagnosis of injury in medico-legal practice.

本文用组织化学和生物化学方法研究了脑/胚胎型肌球蛋白重链异构体（MIIB 2）在四氯化碳（CCl 4）诱导的大鼠肝炎愈合过程中的表达。在细胞增殖和成熟（分化和重建）之间瞬时观察到MIIB2表达增加，表明MIIB2在受损组织的分化或重建中起关键作用。因此，MIIB2的表达似乎是愈合过程中细胞增殖终止的标志，用于损伤组织的修复和再生。在我们的下一步研究中，我们研究了大鼠比目鱼肌组织愈合过程中MIIB2的表达，该组织通过插入18G针或注射BaC12溶液损伤。然而，我们没有发现肌肉组织愈合过程中MIIB2表达的实质性增加。目前尚不清楚肝组织和肌肉组织愈合过程差异的原因，我们可以说我们几乎成功地完成了我们项目的目标。现在，我们将在法医实践中应用MIIB2的表达用于损伤诊断。

项目成果

中島利恵: "実験肝炎治癒過程における脳/胎児型ミオシン重鎖発現に関する研究" 和歌山医学. 49・4. 435-444 (1998)

中岛理惠：“实验性肝炎愈合过程中脑/胎儿肌球蛋白重链表达的研究”和歌山医学49·444（1998）。

Nakashima R.: "Expression of a brain/embryo-type myosin heavy chain in the healing process of experimentally induced hepatitis of rats" Wakayama Igaku. 49-4. 435-444 (1998)

Nakashima R.：“实验诱导大鼠肝炎愈合过程中脑/胚胎型肌球蛋白重链的表达”Wakayama Igaku。