Modulation of signal transduction of human epidermal immunocompetent cells

人表皮免疫活性细胞信号转导的调节

• 批准号: 03454272
• 负责人: TAMAKI Kunihiko
• 金额: $ 3.97万
• 依托单位: Yamanashi Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1991
• 资助国家: 日本
• 起止时间: 1991 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-03454272/
• 关键词: human keratinocyte; human Langerhans cell; HLA-DR; ICAM-1; 1,25(OH)2D3; griseofulvin; CLA; 1,25(OH)_2D3; グリセオフルビン; 1,25(OH)_2D_3; ヒト表皮細胞; ケラチノサイト; ランゲルハンス細胞; HLAーDR; ICAMー1; ビタミンD3

This study was conducted to reveal the mechanisms and modulation of IFN-alpha induced classII MHC and ICAM-1 expression on human keratinocytes(KC) as an extension of our previous study of 1,25(OH)_2D3 modulation of classII MHC on human SCC cell line HEP-2 ( Brit J Dermatol 123 : 333-338,1990).It was found that 1) IFN-alpha induced classII MHC was significantly inhibited by both H-7 and W-7, whereas IFN-alpha induced ICAM-1 was significantly inhibited by H-7 but not by W-7, 2) 1,25(OH)_2D3 inhibited the IFN-alpha induced HLA-DR but not ICAM-1 on human KC, and 3) griseofulvin, an anti-fungal drug, also inhibited the IFN-alpha induced HLA-DR but not ICAM-1 on human KC.Furthermore, it was found that 4) griseofulvin was very effective as a therapeutic reagent for the treatment of plasma cell cheilitis and 5) idiopatic pigmentary purpura, both of which are known as intractable disease.

本研究在1，25（OH）_2D_3对人鳞状细胞癌细胞系HEP-2的MHC Ⅱ类分子调控研究的基础上，进一步探讨了IFN-α诱导人角质形成细胞（KC）表达MHC Ⅱ类分子和ICAM-1的机制（英国皮肤病学杂志123：333- 338，1990）。发现1）IFN-α诱导的II类MHC被H-7和W-7显著抑制，1，25（OH）_2D_3抑制IFN-α诱导的人KC表面HLA-DR，但不抑制ICAM-1; 3）抗真菌药灰黄霉素，还抑制IFN-α诱导的人KC上的HLA-DR，但不抑制ICAM-1。此外，发现4）灰黄霉素作为治疗浆细胞性唇炎和5）特发性色素性紫癜的治疗剂非常有效，这两种疾病都是已知的难治性疾病。

项目成果

Kunihiko Tamaki,Atsushi Saitoh,Yasuo kubota: "1,25-dihydroxyuitaninD3 decrease the interfeun-R(1FN-r)inducea HLA-DR expression but not intercelluler adhesion molecule 1(ICAM-1)on human Rerationoeytes" Reginal jmmunology. 3. 223-227 (1991)

Kunihiko Tamaki、Atsushi Saitoh、Yasuo kubota：“1,25-二羟基维生素 D3 降低人关系细胞上的干扰素-R(1FN-r)诱导 HLA-DR 表达，但不降低细胞间粘附分子 1(ICAM-1)”区域免疫学。

Kunihiko Tamaki, A.Saitoh, N.Yasaka: "Differential effect of griseofulvin on interferon-eta induced HLA-DR and intercellular adhesion molecule-1 expression on human keratinocytes" Brit J Dermatol. 127. 258-261 (1992)

Kunihiko Tamaki、A.Saitoh、N.Yasaka：“灰黄霉素对干扰素 eta 诱导的人类角质形成细胞上的 HLA-DR 和细胞间粘附分子 1 表达的不同影响”Brit J Dermatol。

S.Shimoda,N,Shibagaki,T.Tonaka,Y,Kubota,Y,Hashimoto,K.Tamaki: "Identification of an eqnivalent to murive They-1^+ dendritic efridernel cells in the rat enidermis" Journal of Dermatohyical Suince. 3. 68-71 (1992)

S.Shimoda,N,Shibagaki,T.Tonaka,Y,Kubota,Y,Hashimoto,K.Tamaki：“大鼠表皮中与 murive They-1^ 树突状 efridernel 细胞等价的鉴定”《Dermatohyical Suince 杂志》。

Kunihiko Tamaki,A.Saitoh,N.Yasaka: "Differential effect of griseofulvin on interferon-γ induced HLA-DR and intercellular adhesion molecule-1 expression on human keratinocytes" Brit J Dermatol. 127. 258-261 (1992)

Kunihiko Tamaki、A. Saitoh、N. Yasaka：“灰黄霉素对干扰素 γ 诱导的人类角质形成细胞上的 HLA-DR 和细胞间粘附分子 1 表达的不同影响”Brit J Dermatol。

玉置邦彦: "ランゲルハンス細胞" 皮膚臨床. 35. 1201-1206 (1993)

Kunihiko Tamaki：“朗格汉斯细胞”皮肤病学。 35. 1201-1206 (1993)