Establishment of Langerhans cell-cell line and its spplication for treatment

朗格汉斯细胞系的建立及其治疗应用

• 批准号: 11470179
• 负责人: TAMAKI Kunihiko
• 金额: $ 9.34万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-11470179/
• 关键词: Langerhans cells; cytokine production; esiticesfeird; CD23; Prion; IL-12; Th_1; TGF-β; 樹状細胞; GM-CSF; M-CSF; TNF-α; CD40; マウス; B7-1; B7-2; サイトカイン; 免疫抑制薬; CD40L

We succeeded in getting purified (over 95%) mouse Langerhans cello (LC) and analyzed their characteristics. Although we have not yet succeeded in establishment of LC-all line , we could get the following results.(1) Using purified LC we analyzed the maturing mechanism during LC culture, (2) We also found the effect of GM-CSF, M-CSF, TNF-α, corticasteroid, cyclosporine A and FK506 on LC, (3) The effect of GM-CSF and TGF-β on IL-12 production by LC was studied. (4) CD23 expression by mouse LC and its modulation by cytokines was studied, and (4) Prion related protein expression by LC was found. The induction by TGF-βtreated LC is now under in imoestigation.

我们成功获得了纯化（超过95%）的小鼠朗格汉斯大提琴（LC）并分析了它们的特性。虽然我们尚未成功建立LC-all系，但我们可以得到以下结果。（1）使用纯化的LC我们分析了LC培养过程中的成熟机制，（2）我们还发现了GM-CSF，M-CSF，TNF-α，皮质类固醇，环孢素A和FK506对LC的影响，（3）GM-CSF和TGF-β对IL-12产生的影响 研究了LC。 (4)研究了小鼠LC的CD23表达及其受细胞因子的调节，(4)发现了LC的朊病毒相关蛋白表达。目前正在评估 TGF-β 处理的 LC 的诱导作用。

项目成果

Sugaya M et al.: "Expression of cellular prion-related protein by murine Langerhans cells and Reractinocytes"J Derm Sci. (in press).

Sugaya M 等人：“鼠郎格汉斯细胞和 Reactin 细胞表达细胞朊病毒相关蛋白”J Derm Sci。

Tada Y, et al.: "TGF-β upregulates IL-12 production by mause Langerhans Cell"Eur J Immunol. 31. 294-300 (2001)

Tada Y 等人：“TGF-β 上调小鼠 Langerhans 细胞的 IL-12 产生”Eur J 免疫学杂志 31. 294-300 (2001)

Tada Y,Asahina A,Nakanva K,Tomwa M,Fujiwa H,Tamaki K.: "Granulocyte/macrophage colony stimulating factor inhibits IL-12 production of mouse Langerhans cells"J Immunol. 164. 5113-5119 (2000)

Tada Y、Asahina A、Nakanva K、Tomwa M、Fujiwa H、Tamaki K.：“粒细胞/巨噬细胞集落刺激因子抑制小鼠朗格汉斯细胞的 IL-12 产生”J 免疫学杂志。

Tada Y,Asahina A,Nakanva K,Migook,Tomwa M,Fujiwa H,Tamaki K.: "Transforming growth factor β up regulates CD40-engaged IL-12 production of mouse langerhans cells"Eur J Immunol.. 31. 294-300 (2001)

Tada Y、Asahina A、Nakanva K、Migook、Tomwa M、Fujiwa H、Tamaki K.：“转化生长因子 β 上调小鼠朗格汉斯细胞 CD40 参与的 IL-12 产生”Eur J Nutrition.. 31. 294-300 (2001)

Claudio Solgals,Nakamura K,Asahina A,Tamaki,K: "Functional CD40 liganol is expressed on epidemal Langerhans cells"Journal of Lzukocyte Biology. 66. 281-285 (1999)

Claudio Solgals、Nakamura K、Asahina A、Tamaki、K：“功能性 CD40 木脂醇在表皮朗格汉斯细胞上表达”Lzuk 细胞生物学杂志。