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The role of Japositive denditc cells in organ graht rejection

Ja阳性树突细胞在器官排斥反应中的作用

基本信息

批准号：
61480227
负责人：
TAMAKI Kunihiko
金额：
$ 3.65万
依托单位：
Yamanashi Medical College (1988)The University of Tokyo (1986-1987)
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1986
资助国家：
日本
起止时间：
1986 至 1988
项目状态：
已结题

项目摘要

Althcugh in vitro role of epiderwal Langeilans cells (LC) has been studied extensitiely in vivo rile of LC is still a matter of controversy. In a preions paper, we have estellishied a precise condition to induce delayed-type hypersenoiticrty (DTH) reaction to alloantigens using epidermal cells (EC). In order to clauty the role of LC in vivo, we first used UVB inadiation for the reduction of this DTH reaction. The UVB effect on the induction of cytotoxic T lymphocyte activity (CTL) in vivo and on shingraft nrejection was also mivestigated. Aterthe shin was iisradiated with UVB in vitro, EC were obtainecl. These EC were iinjected via subcutanevus (DC) route and DTH reaction was compared to that mentreated EC. Reduction of DTH seaction who obsewed (fron 62 to 99.1%). CTL activity in these mice was assessed aften in vitrs stimulation. CTL actiorty in miee sensitized with UVB inadiated EC was apprecially seduced. Furtheimore mice scmityod with upb irsadiated EC did not reject the followingon-uvb-inadiated ec rejected auhsegnent shin allograftin an accelewthe feshionNext we used balb/c shin transpleuted onto (cbhxbacr/c)fimice, these jrefted plrin was taken 150 days aftir trimsplantation and ec were made, these ec were injected sc into mormil balb/c mice. The role of lc was studied by depleting lc with anti ta^k serum and c rnplement treatinent. For dth reaction significent reduction was notcd in anti ta^k serum + conplement treated mice. for ctl activity, appaeciable reduction who noted in anti-ta^k + conplement treated group. when uvb wao used reduced tdh reuction and morwel ctl activity was noticed. thus althcugh lc was necessauy for both dth and ctl induction, other mechauism wuned be operatig when uvbv irrediation was used.

尽管表皮朗格兰细胞(LC)在体外的作用已被广泛研究，但在体内LC的作用仍存在争议。在一篇前离子论文中，我们利用表皮细胞(EC)建立了诱导同种异体抗原迟发性超敏反应(DTH)的精确条件。为了明确LC在体内的作用，我们首先使用UVB辐射来还原这种DTH反应。并观察了UVB在体内诱导细胞毒性T淋巴细胞活性(CTL)及对移植瘤排斥反应的影响。在体外用UVB照射胫骨，获得EC。这些EC通过皮下注射(DC)途径注射，并将DTH反应与经皮下注射EC进行比较。潜伏期减少率由62%降至99.1%。在玻璃体刺激下检测小鼠的CTL活性。经UVB照射的EC致敏小鼠的CTL活性明显增强。此外，用UPB诱导的EC更多的小鼠不排斥以下经UVB辐射的同种异体胫骨移植。然后，我们将Balb/c移植到(cbhxbacr/c)小腿上，150天后取这些移植的小腿，制成EC，皮下注射给正常Balb/c小鼠。用抗Ta^k血清耗尽Lc和补体治疗来研究Lc的作用。对DTH反应，抗tak血清+补体处理组小鼠未见明显减少。对于ctl活性，抗ta^k+补体治疗组明显降低。当使用UVB时，WAO的TdH含量和Morwel ctl活性均有所降低。因此，虽然Lc对于DTH和CTL的诱导是必要的，但在使用UVBV照射时，还需要操作其他机制。