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Embryological analysis of ano-rectal malformations, using hereditary pig anomalies.

利用遗传性猪异常对肛门直肠畸形进行胚胎学分析。

基本信息

批准号：
03454297
负责人：
OHKAWA Haruo
金额：
$ 3.01万
依托单位：
University of Tsukuba
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1991
资助国家：
日本
起止时间：
1991 至 1992
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-03454297/
关键词：
Ano-rectal malformations Imperforate anus Cloaca Cloacal plate Comparative anatomy 3-D reconstruction Pig Evolution 顕微画像三次元立体構築 cloacal plate 総排泄腔三次元立体構築法 dorsal cloaca

项目摘要

1) We have successfully maintained 2 pig families with ano-rectal malformations, which we had established by ourselves. We came across multiple occurrence of congenital diaphragmatic hernia in one of the family. We actually succeeded to save 2 pigs with both anomalies. Both diseases had no genetical relations. 2) We successfully obtained pig emryos with ano-rectal malformations, according to well-devised artificial abortions. But we had no success prior to 20 days of gestation according to technical problems. 3) Serial saggital sections were produced in succession of 700-1000 slices for the purpose of three dementional reconstruction on 51 fetuses of 22 to 60 days. 4) Embryological studies have been carried to clarify the early genesis of ano-rectal malformations. The results of three dementional analysis early genesis were presented in the Pacific Association of Pediatric Surgeons in Albuquerque and was presented with the Best Poster Prize. 5) The cause of the anomaly was analysed to be the defect of partition of primitive cloaca into rectum and urogenital sinus. On this occasion, the defect of cloacal plate in the most posterior portion was clarified, as a very important finding. 6) Evolutional aspects of ano-rectal malformations were studied by means of dissections of various vertebrates according to the comparative anatomical procedures.

1)我们成功地维持了2个肛门直肠畸形的猪家庭，这是我们自己建立的。我们发现其中一个家庭多次出现先天性膈疝。我们实际上成功地挽救了两头患有两种异常的猪。这两种疾病没有遗传关系。2)我们成功地获得了猪emryos肛门直肠畸形，根据精心设计的人工流产。但由于技术问题，我们在怀孕20天之前没有成功。3)对51例22 ~ 60天胎儿连续制作矢状面连续切片700-1000片，进行三维重建。4)胚胎学研究已进行澄清肛门直肠畸形的早期发生。三维分析早期起源的结果在阿尔伯克基的太平洋儿科外科医生协会上发表，并获得了最佳海报奖。5)分析其原因是原始泄殖腔与直肠、尿生殖窦分隔不清。在这种情况下，在最后部分的泄殖腔板的缺陷被澄清，作为一个非常重要的发现。6)肛门直肠畸形的进化方面进行了研究，通过解剖各种脊椎动物根据比较解剖程序。