Studies on the Carcinogenesis in Biliary Tract in Relation to Abnormal Pancreatico-biliary Ductal Union.

胰胆管联合异常相关的胆道癌变研究。

• 批准号: 60480299
• 负责人: OHKAWA Haruo
• 金额: $ 2.82万
• 依托单位: Institute of Clinical Medicine, University of Tsukuba
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1985
• 资助国家: 日本
• 起止时间: 1985 至 1986
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-60480299/
• 关键词: Anomalous pancreatico-biliary ductal union; Intestinal motaplasia; Paneth cell differentiated adenocarcinoma; canine model; enterokinase; エンテロキナーゼ; モノクロナール抗体

1. Clonico-pathological Studies on the Carcinogenesis of Biliary Tract in Relation to the Anomalous Pancreatico-biliary Ductal Union (APBDU).The relation between the metaplasia and APBDU, which was one of the most important factors in the carcinogensis of biliary tract carcinogenesis, was studied histologically. In histochemical studies, 175 cases of cholecystitis including 5APBDU, 25 gallbladder carcinoma including 7 APBDU, 35 choledochal carcinoma including 4 APBDU, 9 choledochal dilatation in childhood with APBDU altogether and 20 normal gallbladders. In staining method, Best's carmine after methylation was proven to be very excellent in visualizing Paneth cells properly.In conclusion, (a) The rate of metaplasia increased in proportion to the age. (b) Metaplasia was more often observed in the cases with APBDU. (c) in the positive groupwith metaplasia, differentiated adenocarcinoma was more often seen. In the negative group without metaplasia, undifferentiated adenocarcinoma was more … More often observed. (d) APBDU was significantly well related with metaplasia, and metaplasia was well related with carcinoma especiallywith a particular histological type of carcinoma. However, the direct relation between APBDU and carcinogenesis has not yet been proven in the experiment.2. Pathological Chages of Biliary Tract in the canine Model after Long-term Survival.Fourty-four adult canine models were studied up to 4 and a hlf years. As a result, mucosal metaplasia was proven to be remarkable in 2 years after model formation. This was proven histochemically and electoron microscopically. However, the relation between the biochemichal activity of pancreatic proteinases. In 4 and a half years. tissue atypism was observed, but cellular atypism and carcinogenesis has not yet been proven.3. Detection of Enterokinase as a Key Enzyme in the Ill-effect of Refluxed Pancreatic Juice into the Biliary Tract.Monoclonal antibody of enterokinase was intended to be formed. Two types of antibody were well formed in the process. They are under study for the propriety. Less

1. 胆道癌变与胰胆管异常愈合（APBDU）相关的克隆病理研究。APBDU是胆道癌变的重要因素之一，我们对其与化生的关系进行了组织学研究。在组织化学研究中，胆囊炎175例，包括5例APBDU，胆囊癌25例，包括7例APBDU，胆总管癌35例，包括4例APBDU，合并APBDU的儿童胆总管扩张9例，正常胆囊20例。在染色法中，甲基化后的Best’s胭脂红被证明对Paneth细胞有很好的可视化效果。总而言之，(a)化生率与年龄成正比。(b) APBDU患者多见化生。(c)化生阳性组多见分化性腺癌。未化生的阴性组未分化腺癌多见。(d) APBDU与化生密切相关，化生与癌密切相关，尤其是与特定组织学类型的癌。然而，APBDU与致癌的直接关系尚未在实验中得到证实。犬模型长期生存后胆道的病理改变。对44只成年犬模型进行了长达4年半的研究。结果，在模型形成后2年，粘膜化生被证明是显著的。组织化学和电子显微镜证实了这一点。然而，胰腺蛋白酶的生化活性之间的关系。4年半的时间。观察到组织异型性，但细胞异型性和癌变尚未得到证实。肠激酶是反流胰液进入胆道不良反应的关键酶。制备肠激酶单克隆抗体。在此过程中形成了两种类型的抗体。他们正在研究是否妥当。少

项目成果

大川治夫: 日本小児外科学会雑誌. (1987)

Haruo Okawa：日本小儿外科学会杂志（1987 年）。

Bensallam Khalil: 日本膵胆管合流異常研究会プロシーディング. 8. 92-93 (1985)

Bensallam Khalil：日本胰胆结合研究组论文集。8. 92-93 (1985)。

Haruo Ohkawa et al: "Relation of abnormal pancreatico-biliary ductal union to intestinal metaplasia and carcinogenesis in the biliary tract." Acta Pathol. Jpn.(1987)

Haruo Ohkawa 等人：“胰胆管结合异常与胆道肠化生和癌变的关系。”

Haruo Ohkawa: Acta Pathol.Jpn.(1987)

大川春夫：Acta Pathol.Jpn.(1987)

Bensallam Khalil, Masanori Kikuchi, Kyouichi Nakamura, Haruo Ohkawa, Shigenori Sawaguchi.: "Electronmicroscopic studies on the mucosal metaplasia in anomalous pancreatico-biliary ductal union. (Japanese)" Nihon Suitankan Gouryuu Ijou Kenkyuukaishi. 92-93

Bensallam Khalil、Masanori Kikuchi、Kyouichi Nakamura、Haruo Ohkawa、Shigenori Sawaguchi。：“异常胰胆管联合粘膜化生的电子显微镜研究。（日语）”Nihon Suitankan Gouryuu Ijou Kenkyuukaishi。