Heat-treated autograft, basic research and clinical application

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Among heat-treated bone, bone morphogenegic protein was most preserved in a condition of 70 c, 10 minutes. Under this condition, new cartilge and bone was induced in maximal quantiry. Tendon insertions onto allogeneic bone already treated with several kinds of heat and time condition were studied histologically and biochemically. New bone formation was observed in groups with allografts incubated at 70 c. Tensile strengths examination at graft-insertion revealed surface-demineralization increased their strength up to that of the non-heat-treated group. Heat treatment with 60-70 c for 10 hours preserved bone-inductive activity as indicated by an induction of the mRNAs for alkaline phosphatase, type 1 and type 2 collagens which roughly correlated with the histological changes and Ca content of the implanted tissues.