Process of osteointegration of heat-treated bone graft

热处理骨移植物的骨整合过程

• 批准号: 08671651
• 负责人: SATO Keiji
• 金额: $ 1.47万
• 依托单位: Aichi Medical University (1997-1998)Nagoya University (1996)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08671651/
• 关键词: MRI; compression strength; Remodelling of bone; Osteointergration; Heat-treated bone; 骨密度; 骨強度; 骨改変; 温度処理自家骨

In vivo experiment: Periodical maging modalities such as MRI and Radiogram could not reveal any differences in osteointegrating process between 70℃-treated bone graft and fresh bone graft, especially bone union and marrow changes. Histology disclosed that degree of vascular invasion and osteoblast invasion were less obvious in heat-treated bone graft, Sample of fresh bone graft obviously decreased in its mineral content than that of heat-treated bone graft with significant difference since 2 months after grafting. Compression strength of the sample of fresh bone graft gradually decreased after grafting and reached bottom around 6 month, and recovered to normal strength until 1 year after grafting. On the other hand, that of heat-treated bone had kept its strength until 1 year after grafting and then reached lowest level around 1 year and half. After that point, strength of heat-treated bone recovered until 2 years, This means remodeling of the heat-treated bone is more slow than that o … More f fresh bone graft, In vitro experiment: Quantification of type 1 collagen and osteocalcin in culture medium at 3, 5 and 7th day of culture, produced by osteoblast like cells cultured on the pulverized bone powder of 70℃-treated bone, 120℃-treated bone and fresh bone were done. Quantity of type 1 collagen and osteocalcin produced by cells cultured on l20℃treated bone were markedly increased than that of cells on 70℃-treated bone and fresh bone (osteocalcin p<0.0001, type 1 collagen p<0.0002) with significant differences. Osteocalcin level produced by cells cultured on the l20℃-treated bone decreased gradually. On the other hand, type 1 collagen level increased This means osteoblast, like cells cultured on unfamiliar atmosphere such as l20℃-treated bone were forced to be differentiated and high level of osteocalcin and type 1 collagen should be produced. However, 70℃-treated bone and fresh bone provided natural condition to osteoblast-like cells and these cells could grow with low level of production of osteocalcin and type 1 collagen. Less

体内实验：MRI和X线等定期影像学检查无法显示70℃处理的骨移植物与新鲜骨移植物的骨整合过程有任何差异，特别是骨愈合和骨髓变化。组织学观察显示，热处理骨移植物血管侵袭和成骨细胞侵袭程度不明显，新鲜骨移植物样品中矿物质含量明显低于热处理骨移植物，移植后2个月起差异显着。新鲜骨移植样品的抗压强度在移植后逐渐下降，约6个月达到最低点，直到移植后1年恢复到正常强度。另一方面，热处理骨的强度一直保持到移植后一年，然后在一年半左右达到最低水平。此后，热处理骨的强度直到2年才恢复，这意味着热处理骨的重塑比新鲜骨移植物更慢，体外实验：培养第3、5和7天的培养基中1型胶原和骨钙蛋白的定量，由在70℃处理骨的粉碎骨粉上培养的成骨细胞样细胞产生， 制作120℃处理骨和新鲜骨。 120℃处理骨上培养的细胞产生的1型胶原和骨钙素数量明显高于70℃处理骨和新鲜骨上的细胞（骨钙素p<0.0001，1型胶原p<0.0002），差异显着。 120℃处理的骨上培养的细胞产生的骨钙素水平逐渐下降。另一方面，1型胶原水平增加。这意味着成骨细胞，如在陌生气氛中培养的细胞，例如经过l20℃处理的骨骼，被迫分化，并产生高水平的骨钙素和1型胶原。然而，70℃处理过的骨头和新鲜骨头为成骨细胞样细胞提供了自然条件，这些细胞可以在低水平产生骨钙素和1型胶原蛋白的情况下生长。较少的