Process of osteointegration of heat-treated bone graft

热处理骨移植物的骨整合过程

基本信息

批准号：
08671651
负责人：
SATO Keiji
金额：
$ 1.47万
依托单位：
Aichi Medical University (1997-1998)Nagoya University (1996)
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1996
资助国家：
日本
起止时间：
1996 至 1998
项目状态：
已结题

项目摘要

In vivo experiment: Periodical maging modalities such as MRI and Radiogram could not reveal any differences in osteointegrating process between 70℃-treated bone graft and fresh bone graft, especially bone union and marrow changes. Histology disclosed that degree of vascular invasion and osteoblast invasion were less obvious in heat-treated bone graft, Sample of fresh bone graft obviously decreased in its mineral content than that of heat-treated bone graft with significant difference since 2 months after grafting. Compression strength of the sample of fresh bone graft gradually decreased after grafting and reached bottom around 6 month, and recovered to normal strength until 1 year after grafting. On the other hand, that of heat-treated bone had kept its strength until 1 year after grafting and then reached lowest level around 1 year and half. After that point, strength of heat-treated bone recovered until 2 years, This means remodeling of the heat-treated bone is more slow than that o … More f fresh bone graft, In vitro experiment: Quantification of type 1 collagen and osteocalcin in culture medium at 3, 5 and 7th day of culture, produced by osteoblast like cells cultured on the pulverized bone powder of 70℃-treated bone, 120℃-treated bone and fresh bone were done. Quantity of type 1 collagen and osteocalcin produced by cells cultured on l20℃treated bone were markedly increased than that of cells on 70℃-treated bone and fresh bone (osteocalcin p<0.0001, type 1 collagen p<0.0002) with significant differences. Osteocalcin level produced by cells cultured on the l20℃-treated bone decreased gradually. On the other hand, type 1 collagen level increased This means osteoblast, like cells cultured on unfamiliar atmosphere such as l20℃-treated bone were forced to be differentiated and high level of osteocalcin and type 1 collagen should be produced. However, 70℃-treated bone and fresh bone provided natural condition to osteoblast-like cells and these cells could grow with low level of production of osteocalcin and type 1 collagen. Less

体内实验：定期加工方式（例如MRI和放射线图）无法揭示在70℃处理的骨纹理和新鲜骨纹理之间的骨整合过程中的任何差异，尤其是骨结合和骨髓变化。组织学披露，在热处理骨纹理中，血管浸润和成骨细胞浸润程度不太明显，新鲜骨纹理的样本明显降低了其矿物质的含量，而与热处理的骨纹理相比，自植物后2个月以来，其矿物质的样本显着差异。新鲜骨纹理样品的压缩强度逐渐降低，大约6个月大约达到底部，直到嫁接后1年才能恢复到正常强度。另一方面，经过热处理的骨头一直保持其强度，直到嫁接后1年，然后达到1年半左右的最低水平。在此之后，热处理骨的强度恢复到2年，这意味着对热处理骨的重塑比o…更慢。完成了120℃处理的骨骼和新鲜骨。在L20上培养的细胞产生的细胞产生的1型胶原蛋白和骨钙素的量显着增加，而在70℃处理的骨骼和新鲜骨上的细胞（骨钙素P <0.0001，1型胶原蛋白P <0.0002）具有显着差异。在L20℃处理的骨上培养的细胞产生的骨钙素水平逐渐降低。另一方面，1型胶原蛋白水平增加了成骨细胞，例如在不熟悉的大气中培养的细胞（例如L20℃处理的骨骼）被迫分化，并应产生高水平的骨钙素和1型胶原蛋白。然而，经70℃处理的骨骼和新鲜骨骼为成骨细胞样细胞提供了自然条件，这些细胞可以随着骨钙素和1型胶原蛋白的含量生长而生长。较少的