Molecular Basis of Leukocyte Adhesion Molecules in Early-onset Periodontitis Patients.

早发性牙周炎患者白细胞粘附分子的分子基础。

• 批准号: 03454441
• 负责人: MURAYAMA Yoji
• 金额: $ 4.16万
• 依托单位: Okayama University Dental School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1991
• 资助国家: 日本
• 起止时间: 1991 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-03454441/
• 关键词: early-onset periodontitis; CD11; CD18; aggregation; mRNA

We analyzed the cell-cell adherence related to CD11/CD18 and CD18 mRNA of the individuals with decreased CD11/CD18 expression on the surface of their neutrophils. Epstein Barr virus-transformed B cell lines were developed from one localized juvenile periodontitis (LIP) patient characterized by decreased CD11/CD18 on the neutrophils in peripheral blood and without any remarkable systemic diseases, two siblings with generalized prepubertal periodontitis (GPP) caused from leukocyte adhesion deficiency (LAD), another LIP patient, one localized prepubertal periodontitis (LPP) patient, and two healthy subjects. Adhesion of leukocytes to each other was measured as cluster formation by aggregation assay. The length and the amount of CD18 mRNA expressed in the cell lines were analyzed by Northern blotting using the 32p-labeled CD18 cDNA.The coding region of the mRNA was analyzed by the reverse transcription-polymerase chain reaction method. Base-mismatches between CD18 mRNA and the 32p-labeled RNA probe synthesized from Cd18 cDNA were analyzed by RNase protection assay. In the adherence assay, cells from the LIP patients with decreased CD11/CD18 formed more clusters of smaller size and with fewer cells than those of did the patients and healthy subjects, while the cells from both GPP patients were found not to aggregate and not to form clusters either in the absence or presence of PMA.There were no differences in the length and the amount of mRNA between the LIP patient and the other patients and healthy subjects, while GPP patients expressed a small amount of long mRNA.The whole coding region (2,313 base pairs) was amplified from all of subjects except the GPP Patients, while the 5'-region (1,119 base pairs) was amplified from all subjects. Base-mismatches were detected on the coding region from 965 to 1,450 nucleotides of CD18 mRNA in GPPpatients. No mismatch was detected on other regions of CD18 mRNA in any subject. These results suggest that the LIP patient with an anom

我们分析了与中性粒细胞表面CD 11/CD 18表达降低的个体的CD 11/CD 18和CD 18 mRNA相关的细胞-细胞粘附。Epstein巴尔病毒转化的B细胞系是从一个局限性青少年牙周炎（LIP）患者，其特征是外周血中性粒细胞上的CD 11/CD 18降低，并且没有任何显著的全身性疾病，两个兄弟姐妹患有由白细胞粘附缺陷（LAD）引起的泛发性青春期前牙周炎（GPP），另一个LIP患者，一个局限性青春期前牙周炎（LPP）患者，和两个健康受试者中开发的。通过聚集测定法将白细胞彼此粘附测量为簇形成。用~（32）P标记的CD 18 cDNA进行北方印迹分析，用逆转录-聚合酶链反应（RT-PCR）分析CD 18 mRNA的编码区。RNA酶保护试验分析了CD 18 mRNA与由CD 18 cDNA合成的32 P标记的RNA探针之间的碱基错配。在粘附试验中，来自具有降低的CD 11/CD 18的LIP患者的细胞形成比患者和健康受试者更多的更小尺寸和更少细胞的簇，而两例GPP患者的细胞在PMA存在或不存在时均未聚集成簇，LIP患者与对照组之间mRNA的长度和数量均无差异。除GPP患者外，所有受试者均扩增出整个编码区（2，313个碱基对），而所有受试者均扩增出5 '区（1，119个碱基对）。GP患者CD 18 mRNA编码区965 ~ 1450个核苷酸存在碱基错配。在任何受试者中均未检测到CD 18 mRNA其他区域的错配。这些结果表明，LIP患者与anom

项目成果

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