Molecular Pathology of Periodontal Diseases

研究1.中性粒细胞表面糖蛋白(LF-1家族)异常1)用免疫印迹和流式细胞术检测28例不同类型牙周病患者细胞膜上中性粒细胞表面糖蛋白LFA-1家族β-亚基的表达。LFA-1家族β亚基即使在fMLP刺激后也不能在细胞表面完全表达。3)LFA-1家族β亚基被认为是一种新的牙周病分型的可能标记物。4)LFA-1家族β基因的限制性片段长度多态性(RFLP)分析可能使这种牙周病亚群的基因诊断成为可能。研究2.人类白细胞抗原基因的RFLP分析1)通过检测人类白细胞抗原II类β链基因的变异，探索对特定牙周病亚群进行基因诊断的可能性。用Bam H1对HLADQbeta基因进行限制性片段长度多态性分析，在96名受试者中有4名早发性牙周炎患者存在遗传变异。3)基因变异可作为牙周病的新的基因诊断指标。研究3.早发性牙周炎家系病例报告1)3名青少年牙周炎患者及其父母中的3名除父亲外，其余4人血清抗牙周病细菌免疫球蛋白水平均未升高。2)早发性牙周炎母女和牙周健康的小女儿表现出T细胞活化的宿主防御功能。3)早发性牙周炎母女和牙周健康的小女儿的中性粒细胞对fMLP的趋化活性明显降低。

Study 1. Neutrophil cell surface glycoprotein (LF-1 family) anomaly1) The quantity of LFA-1 family beta-subunit of the neutrophil cell surface glycoprotein expressed on the cell membrane was studied in 28 Patients with various types of periodontal disease by Western blotting and Flow cytometry using the monoclonal antibody against the beta-subunit.2) In patients with early-onset periodontitis, the LFA-1 family beta-subunit was not fully expressed on the cell surface even after the stimulation with fMLP.3) The LFA-1 family beta-subunit is proposed as a possible marker in a novel typing for a certain periodontal disease.4) The restriction fragment length polymorphism (RFLP) analysis of LFA-1 family beta gene may make a gene diagnosis of such a periodontal disease subgroup possible.Study 2. RFLP analysis of HLA genes1) The possibility of gene diagnosis for a specific periodontal disease subgroup was explored by the detection of variations in the HLA class II beta-chain genes.2) The RFLP analysis of HLA-DQbeta gene digested with Bam H1 revealed that genetic variation existed in 4 patients with early-onset periodontitis out of 96 subjects.3) Gene variations may be useful as markers for novel gene diagnosis for a certain subgroup of periodontal disease.Study 3. Case reports of families with early-onset periodontitis1) Out of 3 daughters with juvenile periodontitis and their parents with adult periodontitis, 4 persons except father did not raise the level of serum IgG against periodontopathic bacteria.2) A mother and elder daughter with early-onset periodontitis and periodontally healthy younger daughter revealed host defensive function of T cell activation.3) The neutrophils from a mother and elder daughter with early-onset periodontitis and a periodontally healthy younger daughter showed characteristically reduced chemotactic activity against fMLP.