Molecular Pathology of Periodontal Diseases

研究1。中性粒细胞表面糖蛋白（LF-1家族）异常应用Western blotting和流式细胞术检测了28例不同类型牙周病患者中中性粒细胞表面糖蛋白LFA-1家族β亚基在细胞膜上的表达量。2)在早发性牙周炎患者中，即使fMLP刺激后，LFA-1家族β -亚基在细胞表面也未完全表达。3) LFA-1家族β亚基被认为是某种牙周病新分型的可能标记物。4)通过LFA-1家族β基因的限制性内切片段长度多态性（RFLP）分析，可以对该牙周病亚群进行基因诊断。研究2。HLA基因的RFLP分析1)通过检测HLAⅱ类β链基因的变异，探讨基因诊断特定牙周病亚群的可能性。2)对96例早发性牙周炎患者进行Bam H1酶切hla - dqβ基因的RFLP分析，发现4例早发性牙周炎患者存在遗传变异。基因变异可能是牙周病某一亚群新基因诊断的有用标记。研究3。早发性牙周炎家庭病例报告1)3例青少年牙周炎的女儿及其成人牙周炎的父母，除父亲外，其余4人血清抗牙周炎细菌IgG水平未升高。2)早发性牙周炎的母亲和大女儿以及牙周健康的小女儿表现出T细胞活化的宿主防御功能。3)早发性牙周炎的母亲和大女儿以及牙周健康的小女儿的中性粒细胞对fMLP的趋化活性明显降低。

Study 1. Neutrophil cell surface glycoprotein (LF-1 family) anomaly1) The quantity of LFA-1 family beta-subunit of the neutrophil cell surface glycoprotein expressed on the cell membrane was studied in 28 Patients with various types of periodontal disease by Western blotting and Flow cytometry using the monoclonal antibody against the beta-subunit.2) In patients with early-onset periodontitis, the LFA-1 family beta-subunit was not fully expressed on the cell surface even after the stimulation with fMLP.3) The LFA-1 family beta-subunit is proposed as a possible marker in a novel typing for a certain periodontal disease.4) The restriction fragment length polymorphism (RFLP) analysis of LFA-1 family beta gene may make a gene diagnosis of such a periodontal disease subgroup possible.Study 2. RFLP analysis of HLA genes1) The possibility of gene diagnosis for a specific periodontal disease subgroup was explored by the detection of variations in the HLA class II beta-chain genes.2) The RFLP analysis of HLA-DQbeta gene digested with Bam H1 revealed that genetic variation existed in 4 patients with early-onset periodontitis out of 96 subjects.3) Gene variations may be useful as markers for novel gene diagnosis for a certain subgroup of periodontal disease.Study 3. Case reports of families with early-onset periodontitis1) Out of 3 daughters with juvenile periodontitis and their parents with adult periodontitis, 4 persons except father did not raise the level of serum IgG against periodontopathic bacteria.2) A mother and elder daughter with early-onset periodontitis and periodontally healthy younger daughter revealed host defensive function of T cell activation.3) The neutrophils from a mother and elder daughter with early-onset periodontitis and a periodontally healthy younger daughter showed characteristically reduced chemotactic activity against fMLP.