The Role of Microorganisms in Periodontal Diseases

1.牙周袋内细菌生态学：（1）采用间接免疫荧光法、酶标抗体法和DNA-DNA杂交法（Ebisu，Murayama，Hara）对牙周袋内的特定微生物进行检测。(2)建立了口腔螺旋体的常规培养方法（石川）。（3）采用Wolinella reeta和Streptococcus sanguis混合培养的方法，研究牙周微生物之间的营养关系。并对牙周袋内细菌的继代机制进行了讨论。2.细菌组成及其生物学活性：（1）伴放线放线杆菌的菌落变异与细胞外膜蛋白（菌毛）的存在有关。（2）对生痰月形单胞菌（Selenomonassputigena）和牙龈拟杆菌（Bacteroidegingivalis，Bg）进行了生物学活性测定（Usemoto）。（3）牙周细菌与组织细胞和B的结合特异性 ...更多信息 本文对唾液支原体（Watanabe）和具核梭杆菌（Okamoto）的细菌细胞进行了研究。3. Bg的特异性抗原：Bg 381的抗原通过牙周炎患者的体液免疫应答方式来表征，并纯化（Murayama）。（2）克隆了Bg 381菌毛亚基蛋白（fimbrilin）的编码基因，并进行了序列测定。然后应用于牙周袋内Bg菌株的临床检测（Yoshimura，Suzuki）。（3）构建了Bg 381染色体DNA基因库。克隆产物用于Bg（Abiko）的进一步研究。4.宿主对牙周细菌的反应：（1）乳铁蛋白与牙周袋内细菌的杀菌机制有关（Maita，Endo）。(2)Bg释放的物质通过调节细胞表面受体和内部细胞事件来抑制多形核白细胞的功能（Aono，Maeda）。5.诊断与菌群植物群：（1）检测牙周袋内细菌抗体水平与临床症状及菌群植物群的关系。然后，牙周病的诊断进行了讨论，从结果（原）。（2）从细菌学、生化学、免疫学和临床等方面综合研究牙周病活动性的诊断方法（Ebisu）。少

1.Bacterial ecology in periodontal pockets : (1)The approaches to examination of the specific microorganisms within periodontal pockets were performed through indirect immunofluorescence procedure, enzyme-labeled antibody test and DNA-DNA hybridization method (Ebisu, Murayama, Hara).(2) A method for routine culturing of oral spirochetes was established (Ishikawa). (3)The nutritional relationship among periodontal microorganisms were examined on a mixed culture of Wolinella reeta, a periodontal bacteria, and Streptococcus sanguis. The mechanisms of bacterialsucce ssion in periodontal pockets were discussed. 2.Bacterial components and their biological activities : (1)A colonial variation in Actinobacillus actinomycetemcomitans seems to relate the presence of outermembrane proteins (fimbrite) on the cells (Kato). (2)The biological activities of Selenomonas sputigena and Bacteroides gingivalis(Bg) were examined (Usemoto). (3)Binding specificity of periodontal bacteria to tissue cells and b … More acterial cells were studiedabout Mycoplasma salivarium (Watanabe) and Fusobacterium nucleatum (Okamoto). 3.The specific antigens from Bg : An antigen from Bg 381 was characterized by the manner of humoral immune response in patients with periodontitis and purified (Murayama). (2)The gene encoding the fimbrial subunit protein from Bg 381 (fimbrilin) was cloned and sequenced. Then it was used forclini- cal detection of the Bg strains in periodontal pockets (Yoshimura, Suzuki). (3)Gene banksof the chr omosomal DNA from Bg 381 were constructed. The clone product was used for further studies of Bg (Abiko). 4.Host response against periodontal bacteria : (1)Lactoferrin was related to the bactericidal mechanism in periodontal pockets (Maita, Endo). (2)Bg released sub-stanc es which suppress the functions of polymorphonuclear leukocytes via the modulation of cellsurface re ceptors and internal cellular events (Aono, Maeda). 5.Diagnosis and bacterial flora : (I)The correl ation among antibody levels to bacteria, clinical status, and bacterial flora inperiodontal pockets were examined. Then the diagnosis of periodontal diseases were discussed from the results (Hara). (2)The diagnostic methods for measurement of periodontal disease activity were synthetically searched from the bacterial, biochemical, immunological, and clinical aspects (Ebisu). Less