Study on the calcification by periodental ligament fibroblasts in the periodonta regeneration

在牙周组织再生方面，我们主要研究了成纤维细胞生长因子（FGF）对人牙周膜成纤维细胞（HPLF）增殖和趋化作用的调控以及1 α，25-二羟维生素D3（vit.D3）对HPLF成骨功能的调控。为了了解细胞在相同的生长因子刺激下迁移或增殖的机制，我们感兴趣的是检测人成纤维细胞经历迁移的细胞的不同阶段。对FGF迁移的细胞没有掺入溴脱氧尿苷，表明S期细胞没有被诱导迁移。在迁移的细胞群中没有发现分裂细胞。而迁移的细胞表达c-myc和c-fos mRNA，提示这些细胞已退出细胞周期的G 0期。基于这些结果，我们得出结论，经历迁移的细胞是 ...更多信息 最有可能处于细胞周期的G1期。D3可增强具有成骨样细胞功能的HPLF中碱性磷酸酶（ALP）活性和骨钙素（OC）的产生。评价维生素的作用。D3对HPLF ALP活性和OC产量的影响，我们检测了HPLF培养不同阶段1 α，25-二羟维生素D3受体（VDR）基因表达、ALP活性和OC产量的动力学。随着培养细胞密度的增加，VDR基因表达水平、ALP活性和OC产量增加。Vit. D3在各培养细胞密度下均能显著提高ALP活性和OC产量。然而，维特。D3不增加VDR基因表达水平。当培养细胞密度稀疏时，从多层细胞培养获得的上清液增强细胞中VDR基因的表达。这些稀疏培养期细胞不表达VDR基因和vit。D3对细胞ALP活性和OC产率几乎无影响。因此，如果稀疏细胞先前用培养上清液刺激，则vit. D3大大提高了业主立案法团的生产力。这些结果表明，维生素。D3增强OC生产力，并依赖于HPLF培养细胞中VDR基因表达的水平，并且来自多层细胞的培养上清液含有增加VDR基因表达的因子。少

In the aspect of periodontal regeneration, we focused on the regulation of the proliferation and the chemotaxis by fibroblast growth factor (FGF) and that of osteogenic functions by 1alpha, 25-dihydroxyvitamin D3 (vit.D3) in human periodontal ligament fibroblast (HPLF).Numbers of growth factors have been shown to induce cell migraiton as well as cell proliferation. To understand the mechanisms how the cells were driven to migration or proliferation upon same growth factor stimuli, we are interested in detecting the different phases of the cell of human fibroblasts undergoing migration. Cells migrated against FGF did not incorporate bromodeoxyuridine, suggesting that the cells in S phase were not induced to migrate. No dividing cells were found in the migrated cell population. However, migrated cells were found to express c-myc and c-fos mRNA,suggesting that these cells were exit from G0 phase of the cell cycle. Based upon the results, we conclude that the cells undergoing migration wer … More e most likely in G1 phase of the cell cycle.It has been shown that vit. D3 enhances alkaline phosphatase (ALP) activity and osteocalcin (OC) productivity in HPLF which have osteoblast-like cell functions. To evaluate the role of vit. D3 in ALP activity and OC productivity in HPLF,we examined the kinetics of gene expression of 1alpha, 25-dihydroxyvitamin D3 receptor (VDR), ALP activity and OC productivity at different phases of culture. The level of VDR gene expression, ALP activity and OC productivity were enhanced as culture cell density increased. Vit. D3 significantly enhanced ALP activity and OC productivity in each culture cell density. However, vit. D3 did not increase the level of VDR gene expression. The supernatant obtained from the culture of multilayred cells enhance the expression of the VDR genes in cells when the culture cell density was sparse. These sparse culture phase cells did not express the VDR gene and vit. D3 hardly affect ALP activity and OC productivity in these cells. Thus, if the sparse cells were previously stimulated with the culture supernatant, vit. D3 strongly enhanced OC productivity. These results suggest that vit. D3 enhances OC productivity and depends upon the level of VDR gene expression in HPLF culture cells and that the culture supernatant from multilayred cells contains the factors which increase VDR gene expression. Less