Effects of periodontally related microorganisms on metabolism of human fibroblasts from gingivae with various forms of periodontal disease

本研究的目的是评估人类牙龈纤维块从正常和各种形式的周期性疾病中用于周期性相关微组织的结构成分的十个细胞线准备好了与捐赠者不同的3种周期性疾病类型的供体和捐赠者有健康疾病和捐赠者有健康牙龈的捐赠者：成人周期性疾病， 3个细胞线;快速渐进的周期性病变， 2个细胞线;青少年周期性病变， 1个细胞线;正常的， 2个细胞线。在-80 ° C中，细胞线被富集并储存在细胞外壳中的细菌提取物、十二烷基硫酸盐提取物和脂质体化合物成分#381， B. asaccharolyticus ATCC 25260， Actinobacillus actinomycetemcomitans Y 4和ATCC 29523， Capnocytophaga ochracea S3， Fusobacterium Nucleatum ATCC 25586.各种周期性相关微生物的受影响剂量依赖于纤维块的各种代谢功能：细胞配置、细胞修饰、糖胺聚糖合成、集合合成或DNA合成。一个来自青春期的纤维碎片和一个来自快速渐进期的细胞线在配置中被大大地修改，在细胞缺陷中被抑制，并在DNA合成中被A的组件减少。actinomycescomitans。这些结果建议说，银杏叶纤维块对捐赠者中的细菌提取物有不同的反应。

The purpose of this study is to evaluate the metabolism of human gingival fibroblasts from normal and various forms of periosontal disease for architectural components of periodontally related microorganisms.Ten cell lines of the gingival fibroblast were prepared from donors with different 3 types of periodontal disease and donors with healthy disease and donors with healthy gingiva : adult periodontitis, 3 cell lines ; rapidly progressive periodontitis, 2 cell lines ; juvenile periodontitis, 1 cell line ; and normal, 2 cell lines. The cell lines were proliferated and stored in-80゜C.Sonication extracts, sodium dodecyl sulphate extracts, and lipopolysaccharide components from the cell envelopes of Bacteroides gingivalis #381, B. asaccharolyticus ATCC 25260, Actinobacillus actinomycetemcomitans Y4 and ATCC 29523, Capnocytophaga ochracea S3, Fusobacterium nucleatum ATCC 25586.The architectural components from a variety of periodontally related microorganisms affected dose-dependently on various metabolic functions of fibroblast : cell configuration, cell adherence, glycosaminoglycan synthesis, collagen synthesis, or DNA synthesis. A fibroblast from juvenile periodontitis and a cell line from rapidly progressive periodontitis were greatly deformed in configuration, inhibited in cell adherence, and reduced in DNA synthesis by the components of A. actinomycescomitans. These results suggest that gingival fibroblast have different response to bacterial extracts among disease types of donors.