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Effects of periodontally related microorganisms on metabolism of human fibroblasts from gingivae with various forms of periodontal disease

牙周相关微生物对各种牙周病牙龈成纤维细胞代谢的影响

基本信息

批准号：
60480413
负责人：
MURAYAMA Yoji
金额：
$ 3.84万
依托单位：
OKAYAMA UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1985
资助国家：
日本
起止时间：
1985 至 1986
项目状态：
已结题

项目摘要

The purpose of this study is to evaluate the metabolism of human gingival fibroblasts from normal and various forms of periosontal disease for architectural components of periodontally related microorganisms.Ten cell lines of the gingival fibroblast were prepared from donors with different 3 types of periodontal disease and donors with healthy disease and donors with healthy gingiva : adult periodontitis, 3 cell lines ; rapidly progressive periodontitis, 2 cell lines ; juvenile periodontitis, 1 cell line ; and normal, 2 cell lines. The cell lines were proliferated and stored in-80゜C.Sonication extracts, sodium dodecyl sulphate extracts, and lipopolysaccharide components from the cell envelopes of Bacteroides gingivalis #381, B. asaccharolyticus ATCC 25260, Actinobacillus actinomycetemcomitans Y4 and ATCC 29523, Capnocytophaga ochracea S3, Fusobacterium nucleatum ATCC 25586.The architectural components from a variety of periodontally related microorganisms affected dose-dependently on various metabolic functions of fibroblast : cell configuration, cell adherence, glycosaminoglycan synthesis, collagen synthesis, or DNA synthesis. A fibroblast from juvenile periodontitis and a cell line from rapidly progressive periodontitis were greatly deformed in configuration, inhibited in cell adherence, and reduced in DNA synthesis by the components of A. actinomycescomitans. These results suggest that gingival fibroblast have different response to bacterial extracts among disease types of donors.

本研究旨在评价正常人和各种牙周病患者牙龈成纤维细胞对牙周相关微生物构筑成分的代谢，从3种不同类型牙周病供体和健康牙周病供体以及健康牙龈供体制备了10株牙龈成纤维细胞：成人牙周炎3株;快速进行性牙周炎，2个细胞系;青少年牙周炎，1个细胞系;和正常，2个细胞系。将细胞系增殖并储存在-80 ℃下。来自牙龈拟杆菌#381，B的细胞包膜的超声提取物、十二烷基硫酸钠提取物和脂多糖组分。来自多种牙周相关微生物的结构组分剂量依赖性地影响成纤维细胞的各种代谢功能：细胞构型、细胞粘附、糖胺聚糖合成、胶原合成或DNA合成。青少年牙周炎的成纤维细胞和快速进行性牙周炎的细胞系在结构上发生了很大的变形，抑制了细胞粘附，并减少了DNA合成。伴放线菌这些结果表明，牙龈成纤维细胞对细菌提取物的反应在不同疾病类型的供体之间存在差异。