IDENTIFICATION OF STAINS FROM MEDICO LEGAL ASPECTS

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A study was designed to identify stains derived from plant pigments. Using high performance liquid chromatography (HPLC), 13 species of common weeds were examined, and 12 flavonoids were detected from as low as 100 to 200 ug of dried leaves and from their stains. Identification of plant species should be made, based on the various chromatographic patterns of the flavonoids with different retention indicies, using rutin and rhamnetin as the reference standards.The established method was applied to three crime cases. Stains on clothing from a victim or a suspect which were later on determined as grass stains, and a blade of dried grass taken from another were examined to identify the sites of crimes. The species of each sample was identified based on the characteristic distribution pattern of flavonoids seen on high pergormance liquid chromatography. The scene of the crime was clearly traced from the evidential material and this method was proved to be useful for the criminological application.