IDENTIFICATION OF STAINS FROM MEDICO LEGAL ASPECTS

设计了一项研究来鉴定来自植物色素的染色剂。用高效液相色谱法（HPLC）对13种常见杂草进行了检测，从100 ~ 200 μ g的干叶及其污渍中检出12种黄酮类化合物。以芦丁和鼠李素为对照品，根据不同保留指数的黄酮类化合物的不同色谱模式，对植物种进行鉴别，并将所建立的方法应用于3个犯罪案件。受害者或嫌疑犯衣服上的污渍，后来被确定为草渍，以及从另一个人身上取下的一片干草，被检查以确定犯罪地点。根据高效液相色谱中黄酮类化合物的特征分布模式对各样品进行了种属鉴定。从证据材料中可以清楚地追踪到犯罪现场，这一方法被证明对犯罪学的应用是有用的。

A study was designed to identify stains derived from plant pigments. Using high performance liquid chromatography (HPLC), 13 species of common weeds were examined, and 12 flavonoids were detected from as low as 100 to 200 ug of dried leaves and from their stains. Identification of plant species should be made, based on the various chromatographic patterns of the flavonoids with different retention indicies, using rutin and rhamnetin as the reference standards.The established method was applied to three crime cases. Stains on clothing from a victim or a suspect which were later on determined as grass stains, and a blade of dried grass taken from another were examined to identify the sites of crimes. The species of each sample was identified based on the characteristic distribution pattern of flavonoids seen on high pergormance liquid chromatography. The scene of the crime was clearly traced from the evidential material and this method was proved to be useful for the criminological application.