A STUDY ON SIMULTANEOUS DETERMINATION OF VOLATILE COMPOUNDS IN HUMAN TISSUES

建立了一种可靠、灵敏的方法测定含硫化物、多硫化物、硫硫化物、尼古丁和可替宁的挥发性化合物，以确定最适合进行毒理学分析的组织。在硫化物方面，进行了动物实验，测定了用GC/ECD测定的组织中硫化物和硫代硫化物的浓度。根据这些数据，提出了亚硫酸盐作为硫化氢中毒的指标；死亡病例建议使用血液、肺或脑，存活病例建议使用尿液作为分析材料。针对多硫化物中毒，采用气相色谱和质谱联用技术对多硫化物、硫化物及多硫化物分解产物进行了分析。从动物实验数据中检测出多硫化物和体内组织中硫化物，证实多硫化物中毒。本文简要叙述了两个疑似多硫化物中毒的实际案例。针对烟碱和可替宁，建立了一种可靠、灵敏的GC/MS方法。测定了10例尸检病例组织中尼古丁和可替宁的分布。从实验数据来看，当血液采集不可行时，肌肉是最适合进行毒理学检查的组织。

A reliable and sensitive methods of volatile compounds containing of sulphide, polysulphide, thiosulphide, nicotine and cotinine were developed to determine what was the most suitable tissue for the toxicological analysis.With the respect of sulphide, animal experiments have been made to determine the concentrations of sulphide and thiosulphide in tissues which were measured by GC/ECD.From the data, thiosulphite was proposed for the indicator of hydrogen sulphide poisoning ; blood, lung or brain is recommendable for fatal cases, and urine for survival cases, as materials to be analyzed.With the respect of polysulphide poisoning, polysulphide, sulphide and the decomposed products of polysulphide, were analyzed by GC and GC/MS.From the animal experimental data, detecting of polysulphide and sulphide in body tissues proved the polysulphide poisoning.Two practical cases suspected poisoning by polysulphides are briefly described.With the respect of nicotine and cotinine, a reliable and sensitive method was developed using GC/MS.The distributions of nicotine and cotinine were measured in tissues from 10 autopsy cases. From the experimental data, muscle was the most suitable tissue for toxicological examination, when acquisition of blood is not feasible.