Studies on application of DNA finger-printing to estimation of genetic and environmental variances of some quatitative tarits in ayu, Plecoglossus altivelis

应用DNA指纹图谱估计香鱼、香鱼某些数量性状遗传和环境变异的研究

• 批准号: 04454094
• 负责人: TANIGUCHI Nobuhiko
• 金额: $ 3.84万
• 依托单位: Kochi University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04454094/
• 关键词: ayu; clones; chromosome; mani-pulation; DNA-fingerprinting; quantitative traits; genetic variance; environmental variance

1. DNA fingerprint (DNA-FP) was applied to comfirmation and identification of cloned fish induced by chromosome manupilation in ayu, Plecoglossus altivelis. We examined suitable enzyme/probe combinations and compared radioisotope (RI) and non-radioisotope (Non-RI) labelled probes to obtain clear DNA-FP.The best DNA fingerprintings were obtained by using YNZ 22,33.15 and 33.6 probes with HaeIII,MspI and HinfI restiction enzymes.In these DNA-FPs, the bands were separeted distinctly throughout the membrane in both higher and lower molecular weight regions. The DNA-FP bands obtained with the Non-RI labelled 33.15 probe were as intence and clearly separeted as those obtained with the RI method. All of the fragments were shared among individuals within a same clonal line, but the pattern of detected was significantly different between clonal lines.It was confirmed that DNA-FP is effective for identification of clonal lines. DNA-FP pattern by Non-RI labelled probe was clear enough to be widel … More y applicable to the studies on inbreeding and identification of individuals and families.2. Chromsome manipulated fish could be applied for estimation of heritability of any quantitative traits. Variances are increased in gynogentic fish because of segregation of genotypes in each locus controlling trait, but decreased in clonal fish by reduction of genetic variances. To estimates heritability, by appliing monozygous human twin model for the clonal ayu offsprings which were communally reared from the day hatched, heritability for the clonal population were estimated after the clonal lines were separated by DNA-fingerprinting identification. The values of heritability estimated for performance and morphological traits at 10 month age were relatively high. If the clonal lines were reared separately, tank and density effects (V_<EC1> + V_<EC2> ) may included in the variance between classes (sigma_B^2). The tank effects could be estimated by rearing clonal fish separately in the different tanks as a reference fish.It was concluded that clonal fish could be used as a control fish to estimate heritability of quantitative characters. The fish is also usable to separate common enviromenotal variance within a clonal line (V_<EC1> + V_<EC2> ) and enviromental variance between individuals with in a line (V_<EW> = sigma_W^2). Less

1. 应用DNA指纹图谱（DNA- fp）技术对阿育（ayu, Plecoglossus altivelis）染色体操纵诱导的克隆鱼进行了鉴定。我们检测了合适的酶/探针组合，并比较了放射性同位素（RI）和非放射性同位素（Non-RI）标记的探针，以获得明确的DNA-FP。使用ynz22、33.15和33.6探针与HaeIII、MspI和hinpi酶结合，获得了最佳的DNA指纹图谱。在这些DNA-FPs中，在高分子量和低分子量区域，条带在整个膜上明显分离。Non-RI标记的33.15探针获得的DNA-FP条带与RI方法获得的条带一样强烈和清晰。这些片段在同一无性系内的个体间是共有的，但在不同无性系间的检测模式存在显著差异。证实了DNA-FP对克隆系的鉴别是有效的。Non-RI标记探针的DNA-FP图谱清晰、广泛，更适用于近交研究和个体及家族的鉴定。染色体处理鱼可用于估计任何数量性状的遗传力。在雌核鱼中，由于基因型在每个控制性状上的分离，变异增加，而在无性系鱼中，由于遗传变异减少，变异减小。为了估计遗传力，采用单合子人双胞胎模型，对从孵化之日起共同饲养的无性系进行dna指纹鉴定，分离无性系，估计无性系群体的遗传力。10月龄生产性能和形态性状的遗传力估计值较高。如果将无性系分开饲养，池效应和密度效应（V_<EC1> + V_<EC2>）可能包含在类间方差（sigma_B^2）中。通过在不同的鱼缸中分别饲养克隆鱼作为参考鱼来评估鱼缸效应。结果表明，克隆鱼可作为定量性状遗传力估计的对照鱼。该鱼还可用于分离无性系内的共同环境方差（V_<EC1> + V_<EC2>）和单系内个体之间的环境方差（V_<EW> = sigma_W^2）。少

项目成果

高木基裕,他: "DNAフィンガープリントにおけるリュウキュウアユの遺伝変異保有量と地理的分化" 水産育種. 20. 29-38 (1994)

Motohiro Takagi 等人：“DNA 指纹中琉球香鱼的遗传变异和地理分化”，渔业育种，20. 29-38 (1994)。

H.S.Han et al.: "Application on DNA fingerinting to confirmation of clones in ayu." Nippon Suisan Gakkaishi. 58. 2027-2031 (1992)

H.S.Han 等人：“DNA 指纹识别在香鱼克隆确认中的应用。”

高木 基裕 他: "DNAフィンガープリントにおけるリュウキュウアユの遺伝変異保有量と地理的分化" 水産育種. 20. 29-38 (1994)

Motohiro Takagi 等：“DNA 指纹中琉球香鱼的遗传变异和地理分化”渔业育种 20. 29-38 (1994)。

S.Seki, Y.Asai, T.Satou, and N.Taniguchi: "Difference in the response to water temperature among amphidromous forms, landlocked froms, and their hybrid of ayu, Plecoglossus altivelis." Suisanzoshoku. 42 (3). 459-463 (1994)

S.Seki、Y.Asai、T.Satou 和 N.Taniguchi：“双性性动物、内陆动物及其杂交香鱼 Plecoglossus altivelis 对水温反应的差异。”

Gabriela del Valle,et al.: "Reduced variation of physiological traits in syu clones,Plecoglossus altivelis" Fisheries Science. 60. 523-526 (1994)

Gabriela del Valle 等人：“减少了 syu 克隆中生理性状的变异，Plecoglossus altivelis”渔业科学。