Studies on population genetic informatics and conservation genetics in fish culture and stock enhancement by computer simulation

鱼类养殖和种群增殖中的群体遗传信息学和保护遗传学的计算机模拟研究

• 批准号: 15208018
• 负责人: TANIGUCHI Nobuhiko
• 金额: $ 31.03万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15208018/
• 关键词: DNA marker; population genetics; genetic conservation; broodstock management; wild population; endangered species; aquaculture fish; experimental fish; DNA分子マーカー; 希少種

The management strategy of broodstock must be important in the sustainable production of seed fish not only for aquaculture but also for stock enhancement program where the artificially produced seed fish for release is required to be genetic equivalency with the wild population.In this study, several problems related with conservation of genetic divergence in wild population and broodstock were targeted to obtain useful information concerning with production of qualified seed fish and maintaining appropriate level of genetic divergence which were evaluated by the DNA markers such as microsatellite (MS) and mtDNA polymorphis. Using genotype data of founder stock, the genetic changes in 30^<th> generations were estimated by such indicators of the average number of marker alleles and heterozygosity based on computer simulation. The broodstock management system assisted by DNA markers was also proposed to make a genetically healthy fish seed for aquaculture and to perform the genetically responsible stock enhancement activity.

亲鱼的管理策略对于可持续的鱼种生产至关重要，不仅是为了水产养殖，而且也是为了种群增殖计划，其中人工生产的鱼种要求与野生种群的遗传等效。针对野生群体和亲鱼遗传多样性保护中存在的几个问题，通过微卫星（MS）和线粒体DNA多态性等DNA标记评价，获得了合格的种鱼，并保持了适当的遗传分化水平。利用建立群体的基因型数据，<th>通过计算机模拟，以平均标记等位基因数和平均杂合度为指标，估计了30个世代的遗传变化。还提出了由DNA标记辅助的亲鱼管理系统，以制作遗传健康的水产养殖鱼种，并进行遗传上负责任的种群增殖活动。

项目成果

Isolation and characterization of microsatellite DNA loci for endangered fish, Japanese huchen (Hucho perryi)

濒危鱼类日本虎鱼 (Hucho perryi) 微卫星 DNA 位点的分离和表征

• 发表时间: 2005
• 期刊: Molecular Ecology Notes 5
• 作者: M.Hatakeyama;T.Watanabe;M.Nakajima;H.Kawamura;N.Taniguchi
• 通讯作者: N.Taniguchi

M.Ortega-Villaizan Romo, M.Nakajima, N.Taniguchi: "Isolation and characterization of microsatellite DNA markers in the rare species barfin flounder (Verasper moseri) and its closely related species spotten halibut (Verasper bariegatus)"Molecular Ecology N

M.Ortega-Villaizan Romo、M.Nakajima、N.Taniguchi：“稀有物种鲽鱼 (Verasper moseri) 及其密切相关物种斑点比目鱼 (Verasper bariegatus) 中微卫星 DNA 标记的分离和表征”分子生态学 N

Inheritance mode of fifteen newly developed microsatellite DNA markers in Barfin flounder and spotted halibut

鲽鱼和斑比目鱼新开发的15个微卫星DNA标记的遗传模式

• 发表时间: 2004
• 期刊: Fish Genetics and Breeding Science 34
• 作者: M.M.O.V.Romo;M.Aritaki;M.Nakajima;N.Taniguchi
• 通讯作者: N.Taniguchi

U.Sun, S.Liu, G.Zhao, S.He, Q.Wu, N.Taniguchi, Q.Yu: "Genetic structure of Chinese sucker population Myxocypris asiaticus in the Yangtze River base on mitochondrial DNA marker"Fisheries Science. (受理印刷中). (2004)

U.Sun，S.Liu，G.Zhao，S.He，Q.Wu，N.Taniguchi，Q.Yu：“基于线粒体DNA标记的长江流域中国粘鲫种群遗传结构”水产科学。 （已收到并印刷）（2004 年）。

K.Ohara, N.Taniguchi: "Preliminary study on genetic diversity evaluated by 11 microsatellite markers in Kuromejina Girella leonine and Mejina Girella punctata"Fisheries Science. 69. 861-863 (2003)

K.Ohara、N.Taniguchi：“通过 11 个微卫星标记评估 Kuromejina Girella leonine 和 Mejina Girella punctata 遗传多样性的初步研究”渔业科学。