A study of genetic management of fish population and genetic diversity used micro satellite DNA markers

利用微卫星DNA标记研究鱼类种群遗传管理和遗传多样性

• 批准号: 12306010
• 负责人: TANIGUCHI Nobuhiko
• 金额: $ 27.21万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12306010/
• 关键词: Microsatellite DNA; Population Genetics; Genetic Diversity; Artificial Seed; Experimental Fish; Endangered Species

Following species were examined for the research experiment of genetic divereity in fishes, Ayu and Masu salmon as representative natural population, Koi carp as representative ornamental fish, Surf fish as representative viviparous fish, Guppy as experimental fish, and Ryukyu-Ayu and Matsukaws as endangered species.In these species, microsatellite DNA markers were developed to evaluate genetic diversity.Genetic characteristics such as genetic variability and differences were measured in each species used microsatellite DNA markers.The reduction of genetic diversity calculated from average heterozygosity in cultured populations were observed as the results of inbreeding.From the estimation of effective size of population in each cultured population and seed population for release, the degree of emergency could be estimated in the population of the endangered species.The influence of inbreeding to the population were estimated and methods of remediation was proposed. From the simulation of cross experiment based on the genotype data of parent population, the methods for the genetic remediation in endangered species and conservation of genetic diversity were also proporsed.

在鱼类遗传多样性的研究实验中，对以下物种进行了检查，Ayu和Masu鲑鱼作为代表性的自然种群，锦鲤作为代表性的观赏鱼，冲浪鱼作为代表性的胎生鱼，孔雀鱼作为实验鱼，琉球Ayu和Matsukaws作为濒危物种。在这些物种中，利用微卫星DNA标记技术，对不同物种的遗传多样性进行了研究，并对遗传变异和遗传差异进行了分析微卫星DNA标记。根据平均杂合度计算的养殖群体的遗传多样性的减少被观察到作为近交的结果。从每个养殖群体和用于释放的种子群体中群体的有效大小的估计，通过对濒危物种种群的紧急程度进行评估，估计了近亲繁殖对种群的影响，并提出了补救措施。提出了根据亲本群体基因型数据模拟杂交试验，提出了濒危物种遗传修复和遗传多样性保护的方法。

项目成果

A.Muller-Belecke, K.Ohara, W.Koedprang, N.Taniguchi: "Environmental influence on quantitative traits of naturally isogenic lines of ginbuna (Carassius langsdorfii)"Aquaculture. 203. 251-262 (2002)

A.Muller-Belecke、K.Ohara、W.Koedprang、N.Taniguchi：“环境对 ginbuna (Carassius langsdorfii) 天然等基因系数量性状的影响”水产养殖。

T. Shikano, N. Taniguchi: "Heterosis of neonatal survival on the guppy."Journal of Fish Biology. 60. 715-725 (2002)

T. Shikano, N. Taniguchi：“孔雀鱼新生儿存活的杂种优势”。鱼类生物学杂志。

S.Nugroho,Nobuhiko Taniguchi,K.Kato and S.Miyashita: "Genetic difference among seed populations of greater amberjack used in aquaculture farm of Japan."SUISANZOSHOKU. 48(4). 665-674 (2000)

S.Nugroho、Nobuhiko Taniguchi、K.Kato 和 S.Miyashita：“日本水产养殖场使用的大鰤种子群体的遗传差异”SUISANZOSHOKU。

吉田一範,高木基裕,田中克,谷口順彦: "マイクロサテライトDNA多型によるヒラメ集団と放流用人工種苗の遺伝的変異性と変異。"水産育種. 29. 93-102 (2000)

Kazunori Yoshida、Motohiro Takagi、Masaru Tanaka、Yoshihiko Taniguchi：“由于微卫星 DNA 多态性导致的比目鱼种群和人工苗的遗传变异和变异。” 29. 93-102 (2000)。

K.Sakamoto, W.Koedprang, M.Nakajima, N.Taniguchi: "Themal resistance traits of the clonal silver crucian carp Carrasius langsdorfii and evaluation of these traits using primary culture cells"Fisheries Science. 68. 1029-1033 (2002)

K.Sakamoto、W.Koedprang、M.Nakajima、N.Taniguchi：“克隆银鲫鱼的耐热性状以及使用原代培养细胞对这些性状的评价”《渔业科学》。