A study of genetic management of fish population and genetic diversity used micro satellite DNA markers

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Following species were examined for the research experiment of genetic divereity in fishes, Ayu and Masu salmon as representative natural population, Koi carp as representative ornamental fish, Surf fish as representative viviparous fish, Guppy as experimental fish, and Ryukyu-Ayu and Matsukaws as endangered species.In these species, microsatellite DNA markers were developed to evaluate genetic diversity.Genetic characteristics such as genetic variability and differences were measured in each species used microsatellite DNA markers.The reduction of genetic diversity calculated from average heterozygosity in cultured populations were observed as the results of inbreeding.From the estimation of effective size of population in each cultured population and seed population for release, the degree of emergency could be estimated in the population of the endangered species.The influence of inbreeding to the population were estimated and methods of remediation was proposed. From the simulation of cross experiment based on the genotype data of parent population, the methods for the genetic remediation in endangered species and conservation of genetic diversity were also proporsed.