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Study on simulation of minimal kinship selective breeding for conservation of genetic diversity in successive artificial bred lines in fish

鱼类连续人工繁育系遗传多样性保存的最小亲缘选育模拟研究

基本信息

批准号：
18380111
负责人：
TANIGUCHI Nobuhiko
金额：
$ 10.68万
依托单位：
Fukuyama University (2007)Tohoku University (2006)
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2006
资助国家：
日本
起止时间：
2006 至 2007
项目状态：
已结题

项目摘要

In the fish seed production industry, seed populations with high value of inbreeding coefficient are usually produced because the seeds are reproduced using smaller effective size of brood stock. The genetic changes caused by random genetic drift, inbreeding depression, unconscious selection happened in such inbreeding population may give un-restorable effects on wild population.In this project, we tried to evaluate genetic changes happened in the artificially reproduced seed population for stock enhancement programs of economically important fish species to find up way for genetic management to conserve wild populations based on following actions :1) to develop efficient genetic markers such as DNA polymorphism2) to accumulate the genetic data of population structure and diversity of both the wild and artificially propagated populations3) to develop a simulator to generate genotypes of offspring using genetic data of brood stock in artificial seed production4) to develop a simulator t … More o perform minimal kinship selective breeding for conservation of genetic diversity in the brood stock and wild population5) to make guideline for seed production to preserve genetic diversity in the brood stock and wild populationThe fish species used were representative species from aquacultured species, stock enhanced species and endangered species, such as barfin flounder Verasper moserz; Mekong gigantic catfish Pangasianodon gigas, and sakuramasu Oncorhynchus masou. We collected genotype data of DNA markers for the population genetic analysis and simulation studies for these fishes.Using the soft wares developed in this project for minimal kinship selective breeding, the optimum conditions for selection were examined by repetitive virtual reproduction trials for 30 generations. We found the best condition to conserve the genetic variation being equivalent level of the average number of alleles, and heterozygosity observe in the wild population. This method were useful to conserve the enough level of genetic divergence of brood stock in each species, even if the effective size of brood stock are not enough being less than minimum viable size of population (Ne=50). Less

在鱼类苗种生产中，由于采用较小的有效规格亲鱼进行繁殖，往往会产生近交系数较高的苗种群体。在这类近交群体中发生的随机遗传漂变、近交衰退、无意识选择等遗传变化可能会对野生群体产生不可估量的影响，本项目试图通过对重要经济鱼类人工繁殖的苗种群体中发生的遗传变化进行评估，从以下几个方面寻找保护野生群体的遗传管理方法：1）开发有效的遗传标记如DNA多态性2）积累野生和人工繁殖群体的群体结构和多样性的遗传数据3）开发利用人工种子生产中亲鱼的遗传数据产生后代基因型的模拟器4）开发利用人工种子生产中亲鱼的遗传数据产生后代基因型的模拟器5）开发利用人工种子生产中亲鱼的遗传数据产生后代基因型的模拟器6）开发利用人工种子生产中亲鱼的遗传数据产生后代基因型的模拟器7）开发利用人工种子生产中亲鱼的遗传数据产生后代基因型的模拟器7）开发利用人工种子生产中亲鱼的遗传数据产生后代基因型的模拟器8）开发利用人工种子生产中亲鱼的遗传数据产生后代基因型的模拟器9）开发利用人工种子生产中亲鱼的遗传数据产生后代基因型的模拟器9）开发利用人工种子生产中亲鱼的遗传数据产生后代基因型的模拟器10）开发利用人工种子生产中亲鱼的遗传数据产生后代基因型的模拟器10）开发利用人工种子生产中亲鱼的遗传数据产生后代基因型的模拟器 ...更多信息 o进行最小亲缘选择育种，以保护亲鱼和野生种群的遗传多样性。5）为保护亲鱼和野生种群的遗传多样性制定苗种生产指南。湄公河巨鲶和樱花钩吻鲑。本研究收集了这些鱼类的DNA标记基因型数据，用于群体遗传分析和模拟研究，并利用本项目开发的最小亲缘选择育种软件，通过30个世代的重复虚拟繁殖试验，对最佳选择条件进行了研究。我们发现，保持遗传变异的最佳条件是等位基因平均数和杂合度在野生群体中观察到的水平相当。该方法在有效亲鱼个体数低于最小生存个体数（Ne = 50）时，仍能保持足够的亲鱼遗传分化水平。少

项目成果

期刊论文数量（0）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Genetic diversity of wild Mekong giant catfish Pangasianodo gigas collected from Thailand and Cambodia.

从泰国和柬埔寨采集的野生湄公河巨型鲶鱼的遗传多样性。

DOI：
发表时间：
2007
期刊：
Fisheries Science 73
影响因子：
0
作者：
Ngamsiri T.;Nakajima M.;Sukumanomon S.;Sukumasavin N.,Kamonrat W.;Na-Nakorn U.;Taniguchi N.
通讯作者：
Taniguchi N.

Genetic variation in skipjack tuna Katsuwonus pelamis (Linnaeus) using PCR-RFLP analysis of the mitochondrial DNA D-loop region.

使用线粒体 DNA D 环区域的 PCR-RFLP 分析，了解鲣鱼 Katsuwonus pelamis (Linnaeus) 的遗传变异。