Research of morphological, biochemical, biomechanical change of peripheral nerve in aging

衰老过程中周围神经形态、生化、生物力学变化的研究

• 批准号: 04454379
• 负责人: HIRASAWA Yasusuke
• 金额: $ 2.82万
• 依托单位: Kyoto Prefectural University of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04454379/
• 关键词: Brachial plexus; acryl plastic injection method; microcorrosion cast; scanning electron microscope; extrafascicular capillaries; intrafascicular capillaries; aging; 内血管系; アクリル樹脂注入法; 微細血管鋳型標本; 走査電子顕微鏡; 神経束外血管系; 神経束内血管系; 牽引-損傷

1. Japanese white rabbits were divided into three groups based on their age ; immature groups (4-6 months), mature groups (less than 36 months), aged groups (more than 36 months). The rabbit sciatic nerves were loaded to failure in tension. Load-deformation curves and stress-strain curves were evaluated. The stiffness and tensile strength of imature groups were samller than the mature and aged groups. But there were no significant differences between each groups in mechanical properties.2. The brachial plexus of Japanese white rabbits were surgically exposed under intravenous anesthesia. Each rabbit brachial plexus was stretched laterally up to electrophysiological failure to conduct. The distance between two marks placed on the lower trunks was simultaneously recorded with a video dimensional analysis system. In results, the nerve strain, load and stress at complete conduction block were found to be 8.5%, 2.6N,1MPa, respectively.3. Microvascular architecture of brachial plexus in the Japanese monkey was examined by means of acryl plastic injection method (Ohta et al. 1990) using microcorrosion casts in scanning electron microscope. The extrafascicular capillaries of the brachial plexus were rarely observed, but the intrafascicular capillaries were developped with dense blood supply. Distribution of blood vessels in the brachial plexus was border of segmental blood supply especially under the clavicle.

1.将日本白色家兔按年龄分为3组：未成熟组（4-6月龄）、成熟组（小于36月龄）、老龄组（大于36月龄）。兔坐骨神经在张力下负荷至失效。并绘制了荷载-变形曲线和应力-应变曲线。成熟组的刚度和抗拉强度小于成熟组和老化组。但各组间的力学性能无显著差异.日本白色家兔的臂丛神经在静脉麻醉下手术暴露。将每只家兔臂丛神经横向拉伸至电生理传导失败。用视频尺寸分析系统同时记录两个标记之间的距离。结果表明，完全传导阻滞时的神经应变、负荷和应力分别为8.5%、2.6N和1 MPa.通过丙烯酸塑料注射法（Ohta等，1990），使用扫描电子显微镜中的微腐蚀模型，检查了日本猴臂丛神经的微血管构筑。臂丛神经束外毛细血管少见，束内毛细血管发达，血供密集。臂丛内血管分布是节段性血供的边界，尤以锁骨下为甚。

项目成果

堂埜 秀文: "腕神経叢牽引損傷の生体力学的検討" 日本臨床バイオメカニクス. 14. 91-94 (1993)

Hidefumi Dono：“臂丛神经牵拉损伤的生物力学研究”日本临床生物力学 14. 91-94 (1993)。

堂埜 秀文: "抹梢神経の引張り特性に関する実験的研究" 整形外科バイオメカニクス. 13. 229-232 (1992)

Hidefumi Dono：“周围神经拉伸特性的实验研究”骨科生物力学。13. 229-232 (1992)

柴田 節子: "抹梢神経自家移植術後の微細血管再構築に関する実験的研究" 京都府立医大雑誌. 102. 1101-1116 (1993)

柴田节子：“周围神经自体移植后微血管重建的实验研究”京都府立医科大学学报102. 1101-1116（1993）。

柴田 節子: "末梢神経自家移植術後の微細血管再構築に関する実験的研究" 京都府立医科大学雑誌. 102. 1169-1181 (1993)

Settsuko Shibata：“周围神经自体移植后微血管重建的实验研究”京都府立医科大学学报102。1169-1181（1993）

Setsuko Shibata: "Experimental study on revascularization of peripheral nerve autograft in the rabbit sciatic nerve" Journal of Kyoto Prefectural University of Medicine. 102. 1101-1116

Settsuko Shibata：“兔坐骨神经周围神经自体移植血运重建的实验研究”京都府立医科大学学报。