Differential expression of unknown molecules in neurons after peripheral nerve injury

周围神经损伤后神经元中未知分子的差异表达

• 批准号: 10470310
• 负责人: HIRASAWA Yasusuke
• 金额: $ 8.38万
• 依托单位: Kyoto Prefectural University of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10470310/
• 关键词: Peripheral Nerve; Nerve Regeneration; Transfection; RLCS; Immunohistochemistry; Immunoelectron Microscopy; PCR; cloning

In this study c DNA analysis method, Restriction Landmark c DNA Scanning (RLCS), was applied to elucidate the temporal changes of gene expression in neurons after peripheral nerve injury. The ventral horn (VH) of spinal cord (L3-L5) was dissected at 1, 3, 5, and 7 days after the crush injury of rat sciatic nerves. RCLS profiles were obtained from VH, both from the sciatic nerve crush was performed as well as from the contralateral side where no crush was applied as the control. We compared the profile from the control with five profiles obtained from the VH after injury, and surveyed the temporal change of the expression of these 1991 species of mRNA. 37 of these were shown to change their expression level after injury. 37 genes were classified into 23 patterns. These patterns were classified into three categories ; the continuously up-regulated type (10 species), the transiently up-regulated type (22 species), the down-regulated type (5 species). These complex patterns of gene expression demonstrated after the injury suggest that precious regulation in molecular pathways is required for accomplishing nerve regeneration. Furthermore, the rat homologue of uridine kinase (UK) gene was identified as one of the transiently up-regulated genes. UK mRNA was up-regulated at 7 days after the injury.

本研究应用限制性标记c DNA扫描（RLCS）技术，研究了周围神经损伤后神经元基因表达的时间变化。在大鼠坐骨神经挤压伤后1、3、5和7天，解剖脊髓（L3-L5）前角（VH）。从VH获得RCLS曲线，从进行坐骨神经挤压以及从作为对照的未施加挤压的对侧获得。我们将对照组的图谱与损伤后从VH获得的5个图谱进行了比较，并调查了这些1991种mRNA表达的时间变化。其中37个在损伤后表达水平发生变化。将37个基因分为23种模式。这些模式分为3类：持续上调型（10种），瞬时上调型（22种），下调型（5种）。损伤后这些复杂的基因表达模式表明，实现神经再生需要分子途径的精确调节。此外，尿苷激酶（UK）基因的大鼠同源物被鉴定为瞬时上调基因之一。UK mRNA在伤后7 d表达上调。

项目成果

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