Elucidation of mechanism in pripheral nerve regeneration

周围神经再生的机制是神经生物学的一个基本问题。雪旺细胞在神经再生中的作用可归因于细胞间相互作用、细胞基质相互作用和营养分子。神经营养分子对发育事件有深远的影响，如自然发生的细胞死亡、分化和神经突的生长。由于成纤维细胞的污染和增殖率低，雪旺细胞的原代培养非常困难。因此，我们通过癌基因转移建立了6个雪旺细胞克隆。所有克隆都用S-100蛋白和层粘连蛋白抗体标记，这是雪旺细胞的特异性标记。与成纤维细胞如3T3和rat -2相比，在这些细胞克隆中未检测到纤维连接蛋白和波形蛋白。从大鼠新生坐骨神经培养的rsn -mos条件培养基不仅促进PC12嗜铬细胞瘤细胞的神经突延伸，而且促进大鼠胎儿背根神经节和视网膜的神经突延伸。该活性对NGF和层粘连蛋白抗血清有抗性，不被聚l溶素吸收。

The mechanism underlying the peripheral nerve regeneration is a fundamental problem of neurobiology. Roles of Schwann cells in the nerve regeneration may be ascribe cell-cell interaction, cellmatrix interaction, and trophic molecules. Neurotrophic molecules have a profound influence on developmental events such as naturally occurring cell death, differentiation, and neurite outgrowth. Primary culture of Schwann cells were very difficult because of contamination of fibroblast and its low multification rate. So we established six Schwann cell clones by oncogene transfer. All of the clones were labeled with antibodies to the S-100 protein and the laminin, which are specific markers for Schwann cells. Fibronectin and vimentin were not detected in these cell clones, in contrast to fibroblasts as 3T3 andRat-2. conditioned medium of RSN-moswhich established from rat neonatal sciatic nerve-promoted neurite extension in not only PC12 pheochromocytoma cells but rat fetus dorsal root ganglia and retina. The activity was resistant to NGF and laminin antiserum, not absorbed by poly-L lysin.