Elucidation of mechanism in pripheral nerve regeneration

周围神经再生机制的阐明

• 批准号: 01570844
• 负责人: HIRASAWA Yasusuke
• 金额: $ 1.41万
• 依托单位: Kyoto Prefectural University of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1989
• 资助国家: 日本
• 起止时间: 1989 至 1991
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-01570844/
• 关键词: Schwann cell; Neurotrophic Factor; Laminin; Dorsal Root Genglia; Pheocheomocytoma PC12; Nerve Growth Factor; Retina; ガン遺伝子; 遺伝子導入; 神経芽細胞腫; 細胞融合

The mechanism underlying the peripheral nerve regeneration is a fundamental problem of neurobiology. Roles of Schwann cells in the nerve regeneration may be ascribe cell-cell interaction, cellmatrix interaction, and trophic molecules. Neurotrophic molecules have a profound influence on developmental events such as naturally occurring cell death, differentiation, and neurite outgrowth. Primary culture of Schwann cells were very difficult because of contamination of fibroblast and its low multification rate. So we established six Schwann cell clones by oncogene transfer. All of the clones were labeled with antibodies to the S-100 protein and the laminin, which are specific markers for Schwann cells. Fibronectin and vimentin were not detected in these cell clones, in contrast to fibroblasts as 3T3 andRat-2. conditioned medium of RSN-moswhich established from rat neonatal sciatic nerve-promoted neurite extension in not only PC12 pheochromocytoma cells but rat fetus dorsal root ganglia and retina. The activity was resistant to NGF and laminin antiserum, not absorbed by poly-L lysin.

周围神经再生的机制是神经生物学的基本问题。许旺细胞在神经再生中的作用可能与细胞-细胞相互作用、细胞-基质相互作用和营养分子有关。神经营养分子对发育事件如自然发生的细胞死亡、分化和神经突生长具有深远的影响。雪旺细胞原代培养因成纤维细胞污染和增殖率低而困难。因此，我们建立了六个雪旺细胞克隆癌基因转移。所有克隆都用S-100蛋白和层粘连蛋白的抗体标记，这是雪旺细胞的特异性标记。与成纤维细胞3 T3和Rat-2相比，在这些细胞克隆中未检测到纤维连接蛋白和波形蛋白。从新生大鼠坐骨神经建立的RSN-mos条件培养液不仅能促进PC 12嗜铬细胞瘤细胞的突起生长，而且能促进胎鼠背根神经节和视网膜的突起生长。该活性对NGF和层粘连蛋白抗血清具有抗性，不被多聚L溶素吸收。

项目成果

Hitomi S., Hirasawa Y., Kastumi Y., Amagai T., Sakai Y.: "Differentiation of PC12 pheochromocytoma cells induced by conditioned medium of Schwann cell line." J. Jpn. SOC. Surg. Hand.8(1). 56-59 (1991)

Hitomi S.、Hirasawa Y.、Kastumi Y.、Amagai T.、Sakai Y.：“雪旺细胞系条件培养基诱导 PC12 嗜铬细胞瘤细胞的分化。”

人見 智: "シュワン細胞株による後根神経節と網膜に対する神経栄養効果" 神経組織の成長.再生.移植論文集. 3(1). 58-59 (1991)

Satoshi Hitomi：“雪旺细胞系对背根神经节和视网膜的神经营养作用”神经组织生长3（1）。

Hitomi S., Hirasawa Y., Kastumi Y., Mastura T., Kumamoto K., Sakai Y., Amagai T.: "Differentiation of PC12 cell lines induced by Schwann cell line." J. Jpn. Neural growth, Regeneration and transplantation.2(1). 14-15 (1990)

Hitomi S.、Hirasawa Y.、Kastumi Y.、Mastura T.、Kumamoto K.、Sakai Y.、Amagai T.：“雪旺细胞系诱导的 PC12 细胞系的分化。”

Kastumi, Y., Amagai T., Sakai Y., Hirasawa y., Hitomi S.: "Immortalization of Schwann cells by transformation with oncogene." J. Jpn. Soc. Sug. Hand.7(5). 5-11 (1991)

Kastumi, Y.、Amagai T.、Sakai Y.、Hirasawa y.、Hitomi S.：“通过癌基因转化实现雪旺细胞的永生化。”

人見智: "Rat Schwann細胞培養条件の基礎的検討" 日本整形外科学会誌. Vol.63No.7. S-1173 (1989)

Satoshi Hitomi：“大鼠雪旺细胞培养条件的基础研究”日本骨科学会杂志第 63 卷 S-1173（1989 年）。