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Experimental study on the regeneration of nerve

神经再生的实验研究

基本信息

批准号：
61570723
负责人：
HIRASAWA Yasusuke
金额：
$ 1.28万
依托单位：
Kyoto Prefectural University of Medicine
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1986
资助国家：
日本
起止时间：
1986 至 1988
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-61570723/
关键词：
perineurium compartment crush injury nerve autograft 免疫組織化学 Schwann細胞 Karnovsky method Regeneration of the nerve S-100 protein Shwann cell XIII factor 修復再生過程

项目摘要

A study was made on the regeneration and the permeability of the prineurium following crush injury and nerve grafting to rat sciatic nerves by electron and fluorescence microscopes.1) One and two weeks after injury, fibroblast-like cells showed a concentric arrangement consisting of approximately 10 layers. Four and eight weeks after injury, the flattened cells which had basement menbranes and many pinocytotic vesicles, covered the fascicle consisting of one or two layers. Perineurium was regenerated as normal structure 24 and 36 weeks after injury. In the nerve fascicle, one week after injury, compartment began to be formed by fibroblast-like cells around the regenerated nerve fibers. Four weeks after injury, these cells had basement menbranes and many pinocytotic vesicles. Eight week, these cells showed tight junctions between their processes, and had morphological characteristics of perineurial cells.2) In the regeneration of perineurium, endoneurial fibroblasts were observed to gain morphological characreristics of perineurium, such as micopinocytic vesicles, continuous basement membrane and cell attachment. In the graft and distal nerve trunk, endoneurial fibroblasts proliferated and elongated their processes in the subperineurial sapaces, and differentiated into perineurial cells as the regenerating processes developed. In the proximal and distal suture lines, minifascicles were formed by endoneurial fibroblasts, which encircled bundles of regenrating axons and Schwann cells and differenciated into perineurial cells.

通过电子显微镜和荧光显微镜对大鼠坐骨神经挤压伤和神经移植后神经膜的再生和通透性进行了研究。1）损伤后1周和2周，成纤维细胞样细胞呈同心排列，约10层。损伤后4周和8周，扁平的细胞覆盖一层或两层的束束，有基底膜和许多胞饮小泡。损伤后24周和36周神经束膜再生为正常结构。在神经束中，损伤后1周，再生神经纤维周围的成纤维细胞样细胞开始形成区室。损伤后四个星期，这些细胞具有基底膜和许多胞饮小泡。第八周时，这些细胞的突起之间表现出紧密的连接，并具有神经束膜细胞的形态特征。2）在神经束膜再生过程中，观察到神经内膜成纤维细胞获得了神经束膜形态特征，如小胞泡、连续基底膜和细胞附着。在移植物和远端神经干中，神经内膜成纤维细胞在神经束膜下腔中增殖并延长其过程，并随着再生过程的发展而分化为神经束膜细胞。在近端和远端缝合线中，神经内膜成纤维细胞形成微束，其包围再生轴突和雪旺细胞束，并分化为神经束膜细胞。