Experimental study on the regeneration of nerve

用电镜和荧光显微镜观察了大鼠坐骨神经挤压伤和神经移植后原神经元的再生和通透性。1）损伤后1周和2周，成纤维细胞样细胞呈同心圆排列，约有10层。损伤后4周和8周，细胞扁平，有基底膜和许多吞饮小泡，覆盖在一层或两层的纤维束上。损伤后24周和36周神经束膜再生为正常结构。损伤后1周，神经束中再生神经纤维周围开始有成纤维细胞样细胞形成隔室。损伤后4周，这些细胞有基底膜和许多吞饮小泡。8周时，神经内膜成纤维细胞突起间形成紧密连接，具有神经束膜细胞的形态学特征。2）神经束膜再生过程中，神经内膜成纤维细胞获得神经束膜细胞的形态学特征，如小泡、连续的基底膜和细胞贴壁。在移植物和远端神经干中，神经内膜成纤维细胞在神经束膜下间隙增殖并伸长其突起，并随着再生过程的发展分化为神经束膜细胞。在近端和远端缝合线处，神经内膜成纤维细胞形成小束，它们包围着再生的轴突和雪旺细胞束，并分化为神经束膜细胞。

A study was made on the regeneration and the permeability of the prineurium following crush injury and nerve grafting to rat sciatic nerves by electron and fluorescence microscopes.1) One and two weeks after injury, fibroblast-like cells showed a concentric arrangement consisting of approximately 10 layers. Four and eight weeks after injury, the flattened cells which had basement menbranes and many pinocytotic vesicles, covered the fascicle consisting of one or two layers. Perineurium was regenerated as normal structure 24 and 36 weeks after injury. In the nerve fascicle, one week after injury, compartment began to be formed by fibroblast-like cells around the regenerated nerve fibers. Four weeks after injury, these cells had basement menbranes and many pinocytotic vesicles. Eight week, these cells showed tight junctions between their processes, and had morphological characteristics of perineurial cells.2) In the regeneration of perineurium, endoneurial fibroblasts were observed to gain morphological characreristics of perineurium, such as micopinocytic vesicles, continuous basement membrane and cell attachment. In the graft and distal nerve trunk, endoneurial fibroblasts proliferated and elongated their processes in the subperineurial sapaces, and differentiated into perineurial cells as the regenerating processes developed. In the proximal and distal suture lines, minifascicles were formed by endoneurial fibroblasts, which encircled bundles of regenrating axons and Schwann cells and differenciated into perineurial cells.