Molecular genetic studies on matrix of urinary stone formation

泌尿系结石形成基质的分子遗传学研究

• 批准号: 04454412
• 负责人: KOHRI Kenjiro
• 金额: $ 1.34万
• 依托单位: Nagoya City University (1994)Kinki University (1992-1993)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04454412/
• 关键词: Osteopontin; Urolithiasis; Calprotection; Genetic; Matrix; Calcium; Atheroscrelosis; 尿路結石症; オステオポンチン(OPN); OPN-mRNA; in situ hybridization; northern blot法; 分子生物学

We have researched the pathogenesis of urolithiasis. We detected osteopontin and calprotectin as the matrix of urolithiasis and the expression of mRNA and protein of the materials was noted in the renal distal tubular cells, It is also known that the materials paly an important role in calcifications such as tartar and atheroscrelosis. Both of materials are produced from macrophage, which phagocytoses calcium oxalate and calcium phosphate.Etiology of atherosclerosis is similar to that of urolithiasis. Namely, both diseases mostly exist in middle aged male and old female, and are increasing after World War II attendant upon the European diet. Macrophage, cytokine such as IL-1, TNF et.al were observed in stone forming kidney in rats as well as human being.Based on these findings, it is reasonable to presume that the pathogenesis of urolithiasis is similar to that of atheroscrelosis.

我们研究了尿石症的发病机制。我们检测到骨桥蛋白和钙卫蛋白作为尿石症的基质，并在肾远端肾小管细胞中观察到该材料的mRNA和蛋白的表达，同时还知道该材料在牙垢和动脉粥样硬化等钙化中发挥着重要作用。这两种物质均由巨噬细胞产生，巨噬细胞吞噬草酸钙和磷酸钙。动脉粥样硬化的病因与尿石症相似。也就是说，这两种疾病大多存在于中年男性和老年女性中，并且在二战后随着欧洲饮食的增加而增加。在大鼠和人类的肾结石中均观察到巨噬细胞、IL-1、TNF等细胞因子。基于这些发现，有理由推测尿石症的发病机制与动脉粥样硬化相似。

项目成果

Kenjiro Kohri et al: "Molecular cloning and sequencing of cDNA encoding Urinary stone protein,which is identical to osteopontin" Biochemical and Biophycical Research Communications. 184. 859-864 (1992)

Kenjiro Kohri 等人：“编码尿石蛋白的 cDNA 的分子克隆和测序，与骨桥蛋白相同”《生物化学和生物物理学研究通讯》。

Kenjiro Kohri et al: "Structure and expression of the mRNA encoding urinary stone protein(osteopontin)" The Journal of Biological Chemistry. 268. 15180-15184 (1993)

Kenjiro Kohri 等人：“编码尿石蛋白（骨桥蛋白）的 mRNA 的结构和表达”生物化学杂志。

Tohru Umekawa,Kenjiro Kohri et al: "Expression of osteopontin messenger RNA in the rat kidney on experimental model of renal stone" Biochemistry and Molecular Biology International. 35. 223-230 (1995)

Tohru Umekawa、Kenjiro Kohri 等人：“肾结石实验模型中大鼠肾脏中骨桥蛋白信使 RNA 的表达”国际生物化学与分子生物学。

Tohru Umekawa,Kenjiro Kohri et al: "Glomerular-basemant-membrane antibody and extracorporeal shock wave lithotripsy" The Lancet. 341. -556 (1993)

Tohru Umekawa、Kenjiro Kohri 等：“肾小球基底膜抗体和体外冲击波碎石术”《柳叶刀》。

Tohru Umekawa, Kenjiro Kohri et .al: "Glomerular-basement membrane antibody and extracorporeal shock wave lithotripsy" The Lancet. 341. 556 (1993)

Tohru Umekawa、Kenjiro Kohri 等人：“肾小球基底膜抗体和体外冲击波碎石术”《柳叶刀》。