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Research on High-altitude Adapted Animal Models

高海拔适应动物模型研究

基本信息

批准号：
04454577
负责人：
SAKAI Akio
金额：
$ 3.97万
依托单位：
SHINSHU UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1992
资助国家：
日本
起止时间：
1992 至 1993
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04454577/
关键词：
HYPOXIA High - altitude Pika HPV Isolated lung Cold スンクス Pika 肺高血圧右心室肥大 HPVC

项目摘要

In this project, three studies were performed. First study was to investigate the comparison of susceptibility to high - altitude among three mammalian species. Adult male Sprague Dawley( SD,n=10 )rats, Suncs( n=5 )and Pika( n=6 )were used. The ratio of right to left ventricular weight ( RVW/LVW ), haematocrit ( Ht ) and smooth muscle in media of pulmonary arterioles in Pikas showed significantly low values compared with SD rats. But not differences were observed between SD rats and Suncs. Second, the comparison of susceptibility to high - altitude among rat strains were examined. In this study, six strains of rat i e LEW SSN( n=7 ), F344/N( n=7 ), WM/MS( N=3 ), WKAH/HOK( n=7 ), WF/N( n=3 )and SD( N=27 )were used. To investigate potential differences in lung vascular control among these six rat strains, we measured the pressure response to acute alveolar hypoxia(3%O_2 )or angiotensin 2 injection in isolated perfused lungs. As the result, F334/N showed smallest pressure response to acute alveolar hypoxia and WKAH/HOK showed biggest response to hypoxia. Third, to investigate the effects of cold - exposure on pulmonary pressure response to hypoxia, we studied pressure responses in perfused lungs from rat that had been exposed to a cold environment, and those kept at a normal temperature. The pressure responses of rat lungs to hypoxia just after removal from the cold were significantly lower than that control value, bat the responses began to increase within 12 hrs, and reached a maximum 3days after cessation of cold - exposure. The increase was time - dependent.

在本项目中，进行了三项研究。第一项研究是比较三种哺乳动物对高海拔的敏感性。实验动物为成年雄性SD大鼠(SD，n=10)、Suncs(n=5)和Pika(n=6)。与SD大鼠相比，Pikas大鼠右、左心室重量(RVW/LVW)、红细胞压积(Ht)和肺小动脉中膜平滑肌的比值均显著降低。但SD大鼠与Suncs大鼠之间无明显差异。其次，比较了不同品系大鼠对高原的敏感性。本研究使用了6株大鼠Lew SSN(n=7)、F344/N(n=7)、WM/MS(N=3)、WKAH/HOK(n=7)、WF/N(n=3)和SD(N=27)。为了研究这六个品系大鼠在肺血管控制方面的潜在差异，我们测量了急性肺泡低氧(3%O2)或血管紧张素-2(Ang-2)注射在离体肺的压力反应。结果表明，F334/N对急性肺泡低氧的压力反应最小，WKAH/HOK对低氧的反应最大。第三，为了研究冷暴露对低氧大鼠肺压力反应的影响，我们研究了冷暴露大鼠和常温大鼠肺灌流后的压力反应。大鼠肺脱冷后即刻对低氧的压力反应明显低于对照组，12h内开始升高，3d后达最大值。这一增长是随时间变化的。