RESEARCH FOR EXPRESSION MECHANISMS OF BROAD SPECTRUM PESISTANCE OF TRANSGENIC PLATS AGAINST VIRUS DESEASES
RESEARCH FOR EXPRESSION MECHANISMS OF BROAD SPECTRUM PESISTANCE OF TRANSGENIC PLATS AGAINST VIRUS DESEASES
批准号:
04806007
负责人:
NAMBA Shigetou
金额:
$1.34万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for General Scientific Research (C)
财政年份:
1992
资助国家:
日本
项目状态:
已结题
起止时间:
1992 至 1994
中文摘要
利用定制聚合酶链式反应(CPCR)技术,将黄瓜花叶病毒(CMV)多个分离物和马铃薯属(Potyvieus)多个种的外壳蛋白(CP)基因导入烟草和本氏烟草。转化植株具有高水平的CP表达,并表现出广泛的外壳蛋白介导的对马铃薯Y病毒的抗性。这表明使用有限数量的马铃薯Y病毒来获得对广泛范围的马铃薯Y病毒的保护是可行的。
英文摘要
Coat protein (CP) genes ofseveral isolates of CMV and many species of genus Potyvieus were engineered and transferred into tobacco and Nicotiana benthamiana by custom PCR (CPCR) engineering. Transformed plants gave high livels of CP expression and exhibited broad form coat protein mediated resistance to potyviruses. This suggests the feasibility of using a limited number of potyviruses to get protection against a broad range of potyviruses.
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Ohira, K.: "Nucleotide sequence of the coat protein coding region of tulip breaking virus." Virus Genes. 8. 165-167 (1994)
Ohira, K.:“郁金香破坏病毒外壳蛋白编码区的核苷酸序列。”
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難波 成任: "16S rRNA遺伝子のPCR増幅による植物病原MLOの検出と分類" 植物防疫. 47. 38-45 (1993)
Shigeto Namba:“通过 16S rRNA 基因的 PCR 扩增检测和分类植物致病性 MLO”《植物保护》47. 38-45 (1993)。
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Namba, S.: "Protection of transgenic plants expressing the coat protein gene of watermelon mosaic virus II or zucchini yellow mosaic virus against six potyviruses." Phytopathology. 82 (9). 940-946 (1992)
Namba, S.:“保护表达西瓜花叶病毒 II 或西葫芦黄花叶病毒外壳蛋白基因的转基因植物免受六种马铃薯Y病毒的侵害。”
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S.Namba et al.: "Protection of transgenic plants expressing the coat protein gene of watermelon mosaic virus II or zucchini yellow mosaic virus against six potyviruses." Phytopathology. 82. 940-946 (1992)
S.Namba 等人:“保护表达西瓜花叶病毒 II 或西葫芦黄花叶病毒外壳蛋白基因的转基因植物免受六种马铃薯Y病毒的侵害。”
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Ohira,K.: "Nucleotide sequence of the coat protein coding region of TBV." Virus Genes. 8. 165-167 (1993)
Ohira,K.:“TBV 外壳蛋白编码区的核苷酸序列。”
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共 33 条
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Establishment of Broad Spectrum Resistance Strategy by Switching of Biocencer
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