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FUNDAMENTAL RESEARCH FOR DEVELOPMENT OF PLANT VIRUS MOLECULAR TAXONOMY SYSTEM

植物病毒分子分类系统开发的基础研究

基本信息

批准号：
05304013
负责人：
NAMBA Shigetou
金额：
$ 8.9万
依托单位：
THE UNIVERSITY OF TOKYO
依托单位国家：
日本
项目类别：
Grant-in-Aid for Co-operative Research (A)
财政年份：
1993
资助国家：
日本
起止时间：
1993 至 1994
项目状态：
已结题

项目摘要

The complete nucleotide sequence of the citrus tatter leaf virus (CTLV) strain lily genome was determined. It is 6496 nucleotides long, excluding the 3'terminal poly (A) tract, and contains two putative overlapping open reading frames (ORFs). ORF1 begins at nucleotide position 37 and ends at position 6354, encoding a protein with a molecular weight of 242 kDa. ORF2, which is in a different reading frame that overlaps ORF1, begins at position 4788, ends at position 5750, and can encode a 36 kDa protein. The 242 kDa protein contains two consensus sequences associated with the RNA-dependent RNA polymerase and the NTP-binding helicase. The putative coat protein of CTLV appears to be located in the C-terminal region of the 242 kDa polyprotein. The nucleotide sequence of CTLV-lily has great similarity with apple stem grooving virus (ASGV). These two viruses may be strains of the same virus rather than different viruses. On the other hand, CTLV and ASGV differ in the genome structure from potato virus T (PVT) whose genome structure is homologous to ACLSV.From these observations, it is appropriate to classify PVT in the ACLSV group and to establish a new virus group which includes PVT and ACLSV.This group was accepted and designated the genus Trichovirus at the plenary meeting of the ICTV.CTLV and ASGV continue to be classified in the genus Capillovirus which was established as a genus at the meeting. These classifications should shed light on the evolution of capilloviruses and other related viruses.

测定了柑桔碎叶病毒（CTLV）百合株系全基因组的核苷酸序列。它的长度为6496个核苷酸，不包括3 '末端的poly（A）区，并含有两个假定的重叠开放阅读框架（ORF）。ORF 1起始于核苷酸位置37，终止于位置6354，编码分子量为242 kDa的蛋白质。ORF 2位于与ORF 1重叠的不同阅读框中，起始于位置4788，终止于位置5750，并且可以编码36 kDa的蛋白质。242 kDa的蛋白质含有两个与RNA依赖性RNA聚合酶和NTP结合解旋酶相关的共有序列。CTLV的推定外壳蛋白似乎位于242 kDa多蛋白的C-末端区域。CTLV-百合的核苷酸序列与苹果茎沟病毒（ASGV）有很大的相似性。这两种病毒可能是同一种病毒的毒株，而不是不同的病毒。另一方面，CTLV和ASGV在基因组结构上不同于马铃薯病毒T（PVT），马铃薯病毒T（PVT）的基因组结构与ACLSV同源。将PVT归入ACLSV组是合适的，并建立了一个新的病毒组，包括PVT和ACLSV。ICTV全体会议接受了这一组，并将其命名为毛状病毒属。CTLV和ASGV继续被分类在属中在会议上被确定为属的毛细病毒。这些分类有助于了解毛细病毒和其他相关病毒的进化。