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FUNDAMENTAL RESEARCH FOR DEVELOPMENT OF PLANT VIRUS MOLECULAR TAXONOMY SYSTEM

植物病毒分子分类系统开发的基础研究

基本信息

批准号：
05304013
负责人：
NAMBA Shigetou
金额：
$ 8.9万
依托单位：
THE UNIVERSITY OF TOKYO
依托单位国家：
日本
项目类别：
Grant-in-Aid for Co-operative Research (A)
财政年份：
1993
资助国家：
日本
起止时间：
1993 至 1994
项目状态：
已结题

项目摘要

The complete nucleotide sequence of the citrus tatter leaf virus (CTLV) strain lily genome was determined. It is 6496 nucleotides long, excluding the 3'terminal poly (A) tract, and contains two putative overlapping open reading frames (ORFs). ORF1 begins at nucleotide position 37 and ends at position 6354, encoding a protein with a molecular weight of 242 kDa. ORF2, which is in a different reading frame that overlaps ORF1, begins at position 4788, ends at position 5750, and can encode a 36 kDa protein. The 242 kDa protein contains two consensus sequences associated with the RNA-dependent RNA polymerase and the NTP-binding helicase. The putative coat protein of CTLV appears to be located in the C-terminal region of the 242 kDa polyprotein. The nucleotide sequence of CTLV-lily has great similarity with apple stem grooving virus (ASGV). These two viruses may be strains of the same virus rather than different viruses. On the other hand, CTLV and ASGV differ in the genome structure from potato virus T (PVT) whose genome structure is homologous to ACLSV.From these observations, it is appropriate to classify PVT in the ACLSV group and to establish a new virus group which includes PVT and ACLSV.This group was accepted and designated the genus Trichovirus at the plenary meeting of the ICTV.CTLV and ASGV continue to be classified in the genus Capillovirus which was established as a genus at the meeting. These classifications should shed light on the evolution of capilloviruses and other related viruses.

测定了柑橘碎叶病毒（CTLV）百合株基因组的全核苷酸序列。它有6496个核苷酸长，不包括3'端聚(A)链，并包含两个假定重叠的开放阅读框（orf）。ORF1开始于核苷酸位置37，结束于位置6354，编码一个分子量为242 kDa的蛋白。ORF2位于与ORF1重叠的另一个阅读框中，始于4788位，结束于5750位，可以编码一个36kda的蛋白质。242kda蛋白包含与RNA依赖的RNA聚合酶和ntp结合解旋酶相关的两个一致序列。推测的CTLV外壳蛋白位于242 kDa多蛋白的c端。ctlv -百合的核苷酸序列与苹果茎槽病毒（ASGV）有很大的相似性。这两种病毒可能是同一种病毒的毒株，而不是不同的病毒。另一方面，CTLV和ASGV的基因组结构与马铃薯病毒T （PVT）不同，PVT的基因组结构与ACLSV同源。从这些观察结果来看，将PVT分类为ACLSV组并建立一个包括PVT和ACLSV的新病毒组是合适的。在ICTV的全体会议上，这一组被接受并命名为毛状病毒属。CTLV和ASGV继续被归为Capillovirus属，Capillovirus属是会议确定的一个属。这些分类有助于揭示毛冠病毒和其他相关病毒的进化。