Establishment of Broad Spectrum Resistance Strategy by Switching of Biocencer

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For improving the research, we performed until now, by malting use of plant virus gene, mechanisms of resistance of virus infection were introduced and technique for detection was built. Within plant viruses known, more than 90% are RNA viruses. For gaining high resistance or broad spectrum resistance to these viruses, we performed our research and established related techniques. In this research project, based on our results attained these two years, gene function of Citrus tatter leaf capillovirus and several potyviruses were analyzed. Infectious transcripts were generated by using viral genes or non-coding regions of viral genomes with some modifications. The pathogenicity was analyzed when these infectious transcripts were inoculated to plants. We also constructed plant transformation vector. Several plant virus genes were engeneered into expression vector and then transformed into Ti plasmid. By transformingplant tissues by these constracts, transgenic plants were generated after differentiating and culturing the transformed plant cell. Each trangenic plant line was used for inoculation test with the same virus species or different species. And the level of resistance of each pair of plant line and inoculated virus was analyzed in detail. As a result, several interesting resistance lines were found. To improve broad spectrum resistance of transgenic strategy, more research must be done and might be profound.