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Establishment of Broad Spectrum Resistance Strategy by Switching of Biocencer

Establishment of Broad Spectrum Resistance Strategy by Switching of Biocencer
通过生物中心切换建立广谱耐药策略
批准号:
09556071
负责人:
NAMBA Shigetou
金额:
$9.6万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (B)
财政年份:
1997
资助国家:
日本
项目状态:
已结题
起止时间:
1997 至 1999

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中文摘要
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英文摘要
For improving the research, we performed until now, by malting use of plant virus gene, mechanisms of resistance of virus infection were introduced and technique for detection was built. Within plant viruses known, more than 90% are RNA viruses. For gaining high resistance or broad spectrum resistance to these viruses, we performed our research and established related techniques. In this research project, based on our results attained these two years, gene function of Citrus tatter leaf capillovirus and several potyviruses were analyzed. Infectious transcripts were generated by using viral genes or non-coding regions of viral genomes with some modifications. The pathogenicity was analyzed when these infectious transcripts were inoculated to plants. We also constructed plant transformation vector. Several plant virus genes were engeneered into expression vector and then transformed into Ti plasmid. By transformingplant tissues by these constracts, transgenic plants were generated after differentiating and culturing the transformed plant cell. Each trangenic plant line was used for inoculation test with the same virus species or different species. And the level of resistance of each pair of plant line and inoculated virus was analyzed in detail. As a result, several interesting resistance lines were found. To improve broad spectrum resistance of transgenic strategy, more research must be done and might be profound.
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会议论文
Uehara, Tamaki: "Histopathological studies on two symptom types of phytoplasma associated with lettuce yellows."Ann. Phytopath. Soc. Japari. 65. 465-469 (1999)
Uehara,Tamaki:“对与生菜黄化相关的两种植原体症状类型的组织病理学研究。”Ann。
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通讯作者:
Lu, Xiaoyun: "The genome organization of the broad bean necrosis virus (BBNV)."Archives of Virology. 143. 1335-1348 (1998)
卢晓云:“蚕豆坏死病毒(BBNV)的基因组结构。”病毒学档案。
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通讯作者:
Uehara, T., Tanaka, M., Shiomi, T., Namba, S., Tsuchizaki, T. and Matsuda, I.: "Histopathological studies on two symptom types of phytoplasma associated with lettuce yellows."Ann. Phytopath. Soc. Japan. 65. 465-469 (1999)
Uehara, T.、Tanaka, M.、Shiomi, T.、Namba, S.、Tsuchizaki, T. 和 Matsuda, I.:“与生菜黄化相关的两种植原体症状类型的组织病理学研究”。
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通讯作者:
Nishimura, N., Nakajima, S., Sawayauagi, T. and Namba, S.: "Transmission of Cryptotaenia japonica witches' broom phytoplasma and oinion yellows phytoplasma by Hishimonus sellatus Uhler."Ann. Phytopath. Soc. Japan.. 64. 474-477 (1998)
Nishimura, N.、Nakajima, S.、Sawayauagi, T. 和 Namba, S.:“由菱纹藻 Uhler 传播 Cryptotaenia japonica 扫帚植原体和洋葱黄植原体。”Ann。
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22
    Transgenesis of insect-transmissible plant-pathogenic microorganisms
    • 批准号:
      25660034
    • 项目类别:
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    • 资助金额:
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    • 财政年份:
      2013
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    • 项目类别:
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    • 财政年份:
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    • 依托单位:
    Development of pesticide for phytoplasma disease targeting a virulence factor
    • 批准号:
      23658039
    • 项目类别:
      Grant-in-Aid for Challenging Exploratory Research
    • 资助金额:
      $2.5万
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      2011
    • 负责人:
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    Analysis on the signaling network involved in the host-switching mechanism of phytoplasmas
    • 批准号:
      21248004
    • 项目类别:
      Grant-in-Aid for Scientific Research (A)
    • 资助金额:
      $27.37万
    • 财政年份:
      2009
    • 负责人:
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    • 依托单位:
    海外基金