Establishment of Broad Spectrum Resistance Strategy by Switching of Biocencer

为了进一步提高研究水平，我们利用植物病毒基因进行麦芽化，介绍了植物病毒感染的抗性机制并建立了检测技术。在已知的植物病毒中，超过90%是RNA病毒。为了获得对这些病毒的高抗性或广谱抗性，我们进行了研究并建立了相关技术。本课题在近两年研究成果的基础上，分析了柑桔碎叶冠状病毒和几种多型病毒的基因功能。传染性转录本是利用病毒基因或病毒基因组的非编码区进行修饰而产生的。将这些感染转录本接种到植物上，分析其致病性。我们还构建了植物转化向量。将多个植物病毒基因导入表达载体，转化为Ti质粒。通过这些结构体转化植物组织，将转化后的植物细胞进行分化培养，产生转基因植株。每个转基因株系分别用同一种或不同种的病毒进行接种试验。并详细分析了各对株系和接种病毒的抗性水平。结果，发现了几条有趣的抗性线。为了提高转基因策略的广谱抗性，必须做更多的研究，而且可能是深刻的。

For improving the research, we performed until now, by malting use of plant virus gene, mechanisms of resistance of virus infection were introduced and technique for detection was built. Within plant viruses known, more than 90% are RNA viruses. For gaining high resistance or broad spectrum resistance to these viruses, we performed our research and established related techniques. In this research project, based on our results attained these two years, gene function of Citrus tatter leaf capillovirus and several potyviruses were analyzed. Infectious transcripts were generated by using viral genes or non-coding regions of viral genomes with some modifications. The pathogenicity was analyzed when these infectious transcripts were inoculated to plants. We also constructed plant transformation vector. Several plant virus genes were engeneered into expression vector and then transformed into Ti plasmid. By transformingplant tissues by these constracts, transgenic plants were generated after differentiating and culturing the transformed plant cell. Each trangenic plant line was used for inoculation test with the same virus species or different species. And the level of resistance of each pair of plant line and inoculated virus was analyzed in detail. As a result, several interesting resistance lines were found. To improve broad spectrum resistance of transgenic strategy, more research must be done and might be profound.