Analysis on the signaling network involved in the host-switching mechanism of phytoplasmas

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We investigated the molecular mechanism of phytoplasmal host switching to establish the basis of developing techniques to control the phytoplasma infection. Comparative analyses along with promoter analyses using phytoplasma isolate OY-M and its non-insect-transmissible mutant OY-NIM revealed that ORF3 located in the plasmid of phytoplasmas are involved in insect transmissibility. We developed phytoplasma DNA chip for the first time and performed microarray analysis to study host-specific regulation of phytoplasmal gene expressions. This analysis showed that phytoplasmas adapt to two diverse hosts, plants and insects, by dramatical alterations of their gene expression in response to the host.