Analysis on the signaling network involved in the host-switching mechanism of phytoplasmas

植原体宿主转换机制涉及的信号网络分析

• 批准号: 21248004
• 负责人: NAMBA Shigetou
• 金额: $ 27.37万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-21248004/
• 关键词: 感染・増殖; ファイトプラズマ; 宿主因子; 感染機構

We investigated the molecular mechanism of phytoplasmal host switching to establish the basis of developing techniques to control the phytoplasma infection. Comparative analyses along with promoter analyses using phytoplasma isolate OY-M and its non-insect-transmissible mutant OY-NIM revealed that ORF3 located in the plasmid of phytoplasmas are involved in insect transmissibility. We developed phytoplasma DNA chip for the first time and performed microarray analysis to study host-specific regulation of phytoplasmal gene expressions. This analysis showed that phytoplasmas adapt to two diverse hosts, plants and insects, by dramatical alterations of their gene expression in response to the host.

我们对植原体寄主转换的分子机制进行了研究，为开发植原体感染控制技术奠定了基础。通过对植原体分离物Oy-M及其非昆虫传递性突变体Oy-NIM的启动子分析表明，ORF3位于植原体的质粒中，与昆虫的传递性有关。我们首次开发了植原体DNA芯片，并进行了微阵列分析，以研究植原体基因表达的宿主特异性调控。这一分析表明，植原体通过其基因表达的戏剧性变化来适应植物和昆虫这两种不同的宿主。

项目成果

Significantly low level of small RNA accumulation derived from an encapsidated mycovirus with dsRNA genome

• DOI: 10.1016/j.virol.2009.10.008
• 发表时间: 2010-01-05
• 期刊: VIROLOGY
• 影响因子: 3.7
• 作者: Himeno, Misako;Maejima, Kensaku;Namba, Shigetou
• 通讯作者: Namba, Shigetou

Identification of three MAPKKKs forming a linear signaling pathway leading to programmed cell death in Nicotiana benthamiana.

• DOI: 10.1186/1471-2229-12-103
• 发表时间: 2012-07-08
• 期刊: BMC plant biology
• 影响因子: 5.3
• 作者: Hashimoto M;Komatsu K;Maejima K;Okano Y;Shiraishi T;Ishikawa K;Takinami Y;Yamaji Y;Namba S
• 通讯作者: Namba S

Significance of eukaryotic translation elongation factor 1A in tobacco mosaic virus infection

• DOI: 10.1007/s00705-009-0571-x
• 发表时间: 2010-02
• 期刊: Archives of Virology
• 影响因子: 2.7
• 作者: Y. Yamaji;K. Sakurai;K. Hamada;K. Komatsu;J. Ozeki;A. Yoshida;A. Yoshii;T. Shimizu;S. Namba;T. Hibi

First report of Choanephora rot of ice plant (Mesembryanthemum crystallinum) caused by Choanephora cucurbitarum in Japan

• DOI: 10.1007/s10327-010-0249-y
• 发表时间: 2010-10-01
• 期刊: JOURNAL OF GENERAL PLANT PATHOLOGY
• 影响因子: 1.2
• 作者: Kagiwada, Satoshi;Kayano, Yusuke;Namba, Shigetou
• 通讯作者: Namba, Shigetou

Molecular analysis of genes encoding two different immunodominant membrane proteins in poinsettia branch-inducing phytoplasma (PoiBI)

一品红分枝诱导植原体 (PoiBI) 中编码两种不同免疫显性膜蛋白的基因的分子分析

• 发表时间: 2011
• 作者: Neriya Y.;Maejima K.;Yamaji Y.;Oshima K.;Namba S
• 通讯作者: Namba S