Short-and long-term effects of cytolines on the body water homeostasis-studies in conscious rats

为了阐明白细胞介素-1 β (il -1 β)在控制心血管系统和体液平衡中的作用，我们研究了il -1 β对清醒大鼠饮酒行为、心血管系统和肾功能的影响。(1)饮酒行为- il -1 β (i.p)减弱渗透性口渴和自发饮酒，而低血容量性口渴和血管紧张素II诱导的饮酒不受影响。细胞加氧酶抑制剂吲哚美辛预处理可抑制其抑制反应。(2)心血管功能- il -1 β （i.v.和i.c.v.）引起动脉血压（AP）、心率（HR）和肾交感神经活动（RSNA）升高，并伴有体温升高，这些升高被吲哚美辛预处理阻断。AP、HR和RSNA的最大变化发生在il -1 β (100ng)静脉注射后10 ~ 15min和5ng静脉注射后20 ~ 25min。il -1 β可增加支配肩胛间棕色脂肪组织（BAT）的交感神经的活性。静脉注射il -1 β增加血浆去肾上腺素和ACTH浓度。因此，il -1 β同时激活交感神经流出和ACTH释放。(3)肾功能- il -1 β (i.p)降低每日尿钠和钾排泄量，而尿量不受影响。il -1 β诱导的钠排泄减少在肾去神经大鼠中被消除。将Kainic酸注入第三脑室前腹侧（AV3V）区域，包括终末板血管样器，检测AV3V区域的神经元是否参与il -1 β诱导的反应。il -1 β诱导的尿钠和钾排泄量减少在AV3V区出现kainic酸损伤的大鼠中被消除，而对饮酒和食物摄入的抑制减弱但仍然持续。因此，il -1 β在大脑中似乎有几个解剖学上不同的作用位点，可能涉及不同的受体亚型和机制。少

To elucidate the roles of interleukin 1 beta(IL-1beta), a cytokine with diverse actions, in the control of the cardiocascular system and body fluid balance, the effects of IL-1beta on drinking behavior, cardiovascular system and renal functions were examined in conscious rats.(1) Drinking behavior -- IL-1beta (i.p.) attenuated osmotic thirst and spontaneous drinking, while hypovolemic thirst and angiotensin II - induced drinking were not affected. The inhibitory responses were suppressed by pretreatment with indomethacin, a cytooxygenase inhibitor.(2) Cardiovascular function -- IL-1beta (i.v.and i.c.v.) elicited increases in arterial blood pressure (AP), heart rate (HR) and renal sympathetic nerve activity (RSNA )accompained by an increase in body terperature, which were blocked by pretreatment with indomethacin. The maximum changes in AP, HR and RSNA occurred 10 - 15 min after i.v.injection of IL-1beta (100ng), and 20 - 25 min after i.c.v.injection (5 ng). The activity of the sympathe … More tic efferent nerves innervating the interscapular brown adipose tissue (BAT) was also increased by IL-1beta. Intravenous injection of IL-1beta increased plasma nosradrenaline and ACTH concentrations. Thus, IL-1beta produces simultaneously activation of sympathetic outflow and ACTH release.(3) Renal function --IL-1beta (i.p.) decreased daily urinary sodium and potassium excretion, while urine volume was not affected. The IL-1beta - induced decrease in sodium excretion was abolished in renal denervated rats. Kainic acid was injected into the anterioventral third ventricle (AV3V) region, including the organum vasculosum of the lamina terminalis, to examine whether neurons in the AV3V region are involved in the IL-1beta induced responses. The IL-1beta induced decreases in urinary sodium and potassium excretion were abolished inrats with a kainic acid - induced lesion in the AV3V region, while the suppression in drinking and food intake was attenuated but still persistent. Thus, there appears to be several anatomically distinct sites of action of IL-1beta within the brain, possibly involving different receptor subtypes and mechanisms. Less