Short-and long-term effects of cytolines on the body water homeostasis-studies in conscious rats

为阐明具有多种作用的细胞因子白介素1β(IL-1β)对清醒大鼠饮酒行为、心血管系统和肾功能的影响，探讨了IL-1β在调节心血管系统和体液平衡中的作用。缓解渗透性口渴和自发性饮酒，而低血容量性口渴和血管紧张素II诱导的饮酒不受影响。细胞氧合酶抑制剂吲哚美辛可抑制上述抑制反应。(2)心血管功能--静脉注射和静脉注射IL-1β。可引起动脉压(AP)、心率(HR)和肾交感神经活动(RSNA)的升高，并伴有体温升高，这些效应可被吲哚美辛阻断。AP、HR和RSNA的最大变化出现在静脉注射IL-1β(100 Ng)后10-15分钟和静脉注射IL-1β(5 Ng)后20-25分钟。符号…的活动IL-1β还增加了支配肩峰间棕色脂肪组织(BAT)的更多抽动神经传出神经。静脉注射IL-1β可增加血浆去甲肾上腺素和ACTH浓度。因此，IL-1β可同时激活交感神经流出和ACTH释放。(3)肾功能--IL-1β(I.P.)减少每日尿钠和尿钾排泄，但对尿量无影响。在去神经肾大鼠中，IL-1β引起的钠排泄减少被取消。将红藻氨酸注射到第三脑室前腹区(AV3V)，包括终板的血管细胞器，以检测AV3V区的神经元是否参与了IL-1β的反应。在红藻氨酸诱导的AV3V区损伤的大鼠，IL-1β引起的尿钠和钾排泄减少被取消，而饮水和食物摄取的抑制减弱，但仍持续。因此，在大脑中似乎有几个解剖上不同的IL-1β作用部位，可能涉及不同的受体亚型和机制。较少

To elucidate the roles of interleukin 1 beta(IL-1beta), a cytokine with diverse actions, in the control of the cardiocascular system and body fluid balance, the effects of IL-1beta on drinking behavior, cardiovascular system and renal functions were examined in conscious rats.(1) Drinking behavior -- IL-1beta (i.p.) attenuated osmotic thirst and spontaneous drinking, while hypovolemic thirst and angiotensin II - induced drinking were not affected. The inhibitory responses were suppressed by pretreatment with indomethacin, a cytooxygenase inhibitor.(2) Cardiovascular function -- IL-1beta (i.v.and i.c.v.) elicited increases in arterial blood pressure (AP), heart rate (HR) and renal sympathetic nerve activity (RSNA )accompained by an increase in body terperature, which were blocked by pretreatment with indomethacin. The maximum changes in AP, HR and RSNA occurred 10 - 15 min after i.v.injection of IL-1beta (100ng), and 20 - 25 min after i.c.v.injection (5 ng). The activity of the sympathe … More tic efferent nerves innervating the interscapular brown adipose tissue (BAT) was also increased by IL-1beta. Intravenous injection of IL-1beta increased plasma nosradrenaline and ACTH concentrations. Thus, IL-1beta produces simultaneously activation of sympathetic outflow and ACTH release.(3) Renal function --IL-1beta (i.p.) decreased daily urinary sodium and potassium excretion, while urine volume was not affected. The IL-1beta - induced decrease in sodium excretion was abolished in renal denervated rats. Kainic acid was injected into the anterioventral third ventricle (AV3V) region, including the organum vasculosum of the lamina terminalis, to examine whether neurons in the AV3V region are involved in the IL-1beta induced responses. The IL-1beta induced decreases in urinary sodium and potassium excretion were abolished inrats with a kainic acid - induced lesion in the AV3V region, while the suppression in drinking and food intake was attenuated but still persistent. Thus, there appears to be several anatomically distinct sites of action of IL-1beta within the brain, possibly involving different receptor subtypes and mechanisms. Less