Short-and long-term effects of cytolines on the body water homeostasis-studies in conscious rats

为阐明白细胞介素1 β（IL-1 β）作为一种具有多种作用的细胞因子在心血管系统和体液平衡调控中的作用，本研究观察了IL-1 β对清醒大鼠饮水行为、心血管系统和肾功能的影响。(1)饮酒行为-- IL-1 β（i. p.）减弱渗透性口渴和自发性饮水，而低血容量性口渴和血管紧张素II诱导的饮水不受影响。细胞加氧酶抑制剂吲哚美辛预处理可抑制这种抑制反应。(2)心血管功能- IL-1 β（i. v.和i. c. v.）引起动脉血压（AP）、心率（HR）和肾交感神经活动（RSNA）的增加，并伴有体温升高，这些作用可被消炎痛阻断。静脉注射IL-1 β（100 ng）后10 ~ 15 min和静脉注射IL-1 β（5 ng）后20 ~ 25 min，AP、HR和RSNA的变化最大。交感神经的活动 ...更多信息 支配肩胛间棕色脂肪组织（BAT）的传出神经也被IL-1 β增加。静脉注射IL-1 β可增加血浆诺斯雷那林和ACTH浓度。因此，IL-1 β同时激活交感神经流出和ACTH释放。(3)肾功能-IL-1 β（i. p.）每日尿钠和钾排泄量减少，而尿量不受影响。在去肾神经大鼠中，IL-1 β诱导的钠排泄减少被消除。将红藻氨酸注射到前腹侧第三脑室（AV 3V）区域，包括终板的血管器，以检查AV 3V区域的神经元是否参与IL-1 β诱导的反应。在AV 3V区域有红藻氨酸诱导损伤的大鼠中，IL-1 β诱导的尿钠和钾排泄减少被消除，而饮水和食物摄入的抑制减弱，但仍然持续。因此，在脑内似乎存在几个解剖学上不同的IL-1 β作用部位，可能涉及不同的受体亚型和机制。少

To elucidate the roles of interleukin 1 beta(IL-1beta), a cytokine with diverse actions, in the control of the cardiocascular system and body fluid balance, the effects of IL-1beta on drinking behavior, cardiovascular system and renal functions were examined in conscious rats.(1) Drinking behavior -- IL-1beta (i.p.) attenuated osmotic thirst and spontaneous drinking, while hypovolemic thirst and angiotensin II - induced drinking were not affected. The inhibitory responses were suppressed by pretreatment with indomethacin, a cytooxygenase inhibitor.(2) Cardiovascular function -- IL-1beta (i.v.and i.c.v.) elicited increases in arterial blood pressure (AP), heart rate (HR) and renal sympathetic nerve activity (RSNA )accompained by an increase in body terperature, which were blocked by pretreatment with indomethacin. The maximum changes in AP, HR and RSNA occurred 10 - 15 min after i.v.injection of IL-1beta (100ng), and 20 - 25 min after i.c.v.injection (5 ng). The activity of the sympathe … More tic efferent nerves innervating the interscapular brown adipose tissue (BAT) was also increased by IL-1beta. Intravenous injection of IL-1beta increased plasma nosradrenaline and ACTH concentrations. Thus, IL-1beta produces simultaneously activation of sympathetic outflow and ACTH release.(3) Renal function --IL-1beta (i.p.) decreased daily urinary sodium and potassium excretion, while urine volume was not affected. The IL-1beta - induced decrease in sodium excretion was abolished in renal denervated rats. Kainic acid was injected into the anterioventral third ventricle (AV3V) region, including the organum vasculosum of the lamina terminalis, to examine whether neurons in the AV3V region are involved in the IL-1beta induced responses. The IL-1beta induced decreases in urinary sodium and potassium excretion were abolished inrats with a kainic acid - induced lesion in the AV3V region, while the suppression in drinking and food intake was attenuated but still persistent. Thus, there appears to be several anatomically distinct sites of action of IL-1beta within the brain, possibly involving different receptor subtypes and mechanisms. Less