Central mechanism for the control of osmo/sodium balance under a salt-loading condition
盐负荷条件下控制渗透压/钠平衡的中心机制
基本信息
- 批准号:11470019
- 负责人:
- 金额:$ 8万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (B)
- 财政年份:1999
- 资助国家:日本
- 起止时间:1999 至 2001
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Changes in adaptive responses following central salt loading have been examined with electrophysiological, neurochemical, and immunohistochemical methods. The results obtained are as follows :(1)The effects of infusion of hypertonic saline (HS) into the lateral cerebroventricle (icv) of conscious freely-moving rats on arterial blood pressure (ABP), heart rate (HR), and renal sympathetic nerve activity (RSNA) were examined. ABP and RSNA increased and decreased, respectively, in a concentration-dependent manner, while HR did not change.Endogenous vasopressin might be involved in salt loading-induced inhibition in the RSNA via a V_1, receptor.(2) Next, the effects of central HS on ABP and HR in conscious rats transgenic for the metallothionin-vasopressin fusion gene (Tg), which had been provided by Dr. Yutaka Oiso (Dept. Internal Medicine, Nagoya University, School of Medicine), were examined, (a) Sprague-Dawley (SD) rats were used as the control animal. No significant differences in basa … More l ABP and HR were found between SD and Tg rats. The pressor response to central HS was significantly larger in Tg than in SD rats. (b) The property of neurons in the brain, which responds to icv administration of HS was examined using Fos-like immunoreactivity (FLI) as a marker of neuronal activity in conscious Tg and SD rats. FLI expression significantly increased in the median preoptic nucleus (MnPO), the organum vasculosum laminae terminalis (OVLT), the paraventricular nucleus (PVN) and supraoptic nucleus (SON) of the hypothalamus, and the area postrema (AP), but not in the subfornical organ (SFO), to 0.3 M HS administration. It is noteworthy that FLI expression was significantly lower in Tg rats in the PVN, SON, AP, and SFO in response to 0.15 M NaCl solution than in SD rats, (c) Vasopressin V, receptor antagonist (OPC-21268, Otsuka, Japan, 250 mg/kg, icv) did not affect basal ABP and HR but elicited a tendency to increase basal FLI expression in the PVN and SON, close to that in SD rats.(3) To elucidate the neurochemical mechanisms responsible for the cardiovascular responses induced by central HS, the PVN region was directly perfused with HS by using an in vivo microdialysis technique. We then measured the extracellular concentration of noradrenaline (NE), nitric oxide metabolites (NOx1), and glutamate in the PVN region in conscious rats along with ABP and HR, AMP, HR, NE, NOx1, and glutamate increased by the perfusion of HS. Dizocilpine, a noncompetitive antagonist of the N-metyl-D-aspartate (NMDA) receptor, attenuated the increase of ABP and HR, although 6-cyano-7-nitroquinoxaline-2, 3-dione, an antagonist of the non-NMDA receptor, did not affect ABP or HR. Our results show that local perfusion of the hypothalamic PVN region with HS elicits a local release of various biological active substances ; among them, glutamate acts via the NMDA-type glutamate receptor to produce cardiovascular responses. Less
采用电生理、神经化学和免疫组织化学方法研究了中央盐负荷后适应性反应的变化。结果如下:(1)观察清醒自由活动大鼠侧脑室(icv)灌注高渗生理盐水(HS)对动脉血压(ABP)、心率(HR)和肾交感神经活动(RSNA)的影响。ABP和RSNA分别呈浓度依赖性升高和降低,而HR没有变化。内源性抗利尿激素可能通过V_1受体参与盐负荷诱导的RSNA抑制。(2)接下来,观察中央HS对转入金属硫蛋白-抗利尿素融合基因(Tg)的清醒大鼠ABP和HR的影响,该基因由Yutaka Oiso博士(名古屋大学医学院内科)提供。(a)以SD大鼠为对照动物。SD大鼠与Tg大鼠ABP和HR差异无统计学意义。Tg大鼠对中枢HS的压力反应明显大于SD大鼠。(b)利用fos样免疫反应性(FLI)作为清醒Tg和SD大鼠神经元活动的标志,研究脑内神经元对icv给药HS的反应特性。在0.3 M HS给药时,FLI在下丘脑视前正中核(MnPO)、末梢血管膜器(OVLT)、室旁核(PVN)和视上核(SON)以及后脑区(AP)的表达显著增加,但在皮质下器官(SFO)中表达不明显。值得注意的是,在0.15 M NaCl溶液下,Tg大鼠在PVN、SON、AP和SFO中FLI的表达明显低于SD大鼠。(c)受体拮抗剂抗利尿素V (OPC-21268, Otsuka, Japan, 250 mg/kg, icv)不影响基础ABP和HR,但在PVN和SON中有增加基础FLI表达的趋势,与SD大鼠相近。(3)为了阐明中央HS诱导心血管反应的神经化学机制,我们采用体内微透析技术直接向PVN区灌注HS。然后我们测量清醒大鼠PVN区去甲肾上腺素(NE)、一氧化氮代谢物(NOx1)和谷氨酸的细胞外浓度,以及ABP和HR、AMP、HR、NE、NOx1和谷氨酸的浓度随HS灌注而升高。非NMDA受体拮抗剂6-氰基-7-硝基喹啉- 2,3 -二酮对ABP和HR没有影响,但n-甲基-d -天冬氨酸(NMDA)受体的非竞争性拮抗剂二唑西平能降低ABP和HR的升高。结果表明,HS局部灌注下丘脑PVN区可引起多种生物活性物质的局部释放;其中,谷氨酸通过nmda型谷氨酸受体产生心血管反应。少
项目成果
期刊论文数量(49)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Shirasaka,T.et al.: "Sympathetic and cardiovascular actions of orexins in conscious rats."Am.J.Physiol.. 277. R1780-R1785 (1999)
Shirasaka,T.et al.:“清醒大鼠中食欲素的交感神经和心血管作用。”Am.J.Physiol.. 277. R1780-R1785 (1999)
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- 影响因子:0
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Kunitake, T., Kannan, H.: "Discharge pattern of renal sympathetic nerve activity in the conscious rat : Spectral analysis of integrated activity"J. Neurophysiol.. 84. 2859-2867 (2000)
Kunitake,T.,Kannan,H.:“清醒大鼠肾交感神经活动的放电模式:综合活动的光谱分析”J。
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- 影响因子:0
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Jin, Q.-H.et al.: "Cardiovascular changes indued by central hypertonic saline are accompanied by glutamate release in awake rats"Am. J. Physiol.. 281. R1224-R1231 (2001)
Jin, Q.-H.等人:“清醒大鼠中中心高渗盐水引起的心血管变化伴随着谷氨酸释放”Am。
- DOI:
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- 影响因子:0
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- 通讯作者:
Jin, Q.-H.et al.: "Cardiovascular changes induced by central hypertonic saline are accompanied by glutamate release in awake rats"Am. J. Physiol.. 281. R1224-R1231 (2001)
Jin, Q.-H.等人:“中枢高渗盐水引起的心血管变化伴随着清醒大鼠中谷氨酸的释放”Am。
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- 影响因子:0
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Kato, K., Shirasaka, T., Kunitake, T., Hanamori, T. & Kannan, H.: "Participation of arterial baroreceptors input and peripheral vasopressin in the suppression of renal sympathetic nerve activity induced by central salt loading in conscious rats"J. Auton.
加藤,K.,白坂,T.,国竹,T.,花森,T.
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- 影响因子:0
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KANNAN Hiroshi其他文献
KANNAN Hiroshi的其他文献
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{{ truncateString('KANNAN Hiroshi', 18)}}的其他基金
Modulation of central sodium / osmo-homeostatic mechanisms by novel peptides-Integrative studies
新型肽对中枢钠/渗透压稳态机制的调节-综合研究
- 批准号:
17390061 - 财政年份:2005
- 资助金额:
$ 8万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Integrative studies on central osmolality receptive mechanisms : Modification by novel peptides and environmental stress
中心渗透压接受机制的综合研究:新型肽和环境应激的修饰
- 批准号:
14370024 - 财政年份:2002
- 资助金额:
$ 8万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Development of a measurement system of autonomic nervous activity in genetic pathological-model mice
遗传病理模型小鼠自主神经活动测量系统的研制
- 批准号:
10557009 - 财政年份:1998
- 资助金额:
$ 8万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Humoral-sympathetic interaction in the adaptive regulation of body-fluid balance under central salt loading : involvement of arterial baroreceptor and vasopressin.
中心盐负荷下体液平衡适应性调节中的体液-交感神经相互作用:动脉压力感受器和加压素的参与。
- 批准号:
09670073 - 财政年份:1997
- 资助金额:
$ 8万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Role of the renal sympathetic nerve activity in determining salt sensitivity.
肾交感神经活动在确定盐敏感性中的作用。
- 批准号:
07670088 - 财政年份:1995
- 资助金额:
$ 8万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Short-and long-term effects of cytolines on the body water homeostasis-studies in conscious rats
细胞素对清醒大鼠体内水稳态的短期和长期影响研究
- 批准号:
04807010 - 财政年份:1992
- 资助金额:
$ 8万 - 项目类别:
Grant-in-Aid for General Scientific Research (C)
Brain peptides : central regulation of the cardiovascular, renal and autonomic nervous functions in conscious rats
脑肽:清醒大鼠心血管、肾和自主神经功能的中枢调节
- 批准号:
02807018 - 财政年份:1990
- 资助金额:
$ 8万 - 项目类别:
Grant-in-Aid for General Scientific Research (C)
Roles of neuropeptides in the neural control of cardiovascular function.
神经肽在心血管功能的神经控制中的作用。
- 批准号:
62570078 - 财政年份:1987
- 资助金额:
$ 8万 - 项目类别:
Grant-in-Aid for General Scientific Research (C)
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