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A STUDY ON VIVIDNESS OF UNDERWATER OPTICAL MICROSCOPE IMAGE WITH HIGH MAGNIFICATION AND STEREOSCOPIC DISPLAY SYSTEM

水下光学显微镜高倍立体显示图像清晰度研究

基本信息

批准号：
05452228
负责人：
TAZAWA Eiichi
金额：
$ 2.3万
依托单位：
HIROSHIMA UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1993
资助国家：
日本
起止时间：
1993 至 1994
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05452228/
关键词：
MICROSCOPIC OBSERVATION OPTICAL MICROSCOPE UNDERWATER IMAGE GRAPHICS WORKSTATION STEREOSCOPIC DISPLAY LENS WHICH CAN BE IMMERSED IN LIQUID IMAGE PROCESSING

项目摘要

Optical microscopes have the disadvantage that only a limited depth can be focussed. In order to cope with disadvantage, the stereoscopic display system using optical microscopes has been developed in this study. That is, by using image processing techniques, focussed areas are extracted from multiple images taken on slightly different depths, and a set of stereoscopic images is then synthesized using these focussed areas. This study aims to solve problems when the system is used in practice and to reduce the speed of image processing in order to observe changes in morphology of cement minerals during hydration of cement minerals.The time needed for extracting and processing image was able to be reduced to one third by introducing graphics workstation purchased in this study. Use of objective lens which could be immersed in liquid solved the problems of both waterproofing of abjectivelens and fixing of samples in water.Concerning fixing of samples, a box was made with silicone rubber on a slide glass before sample and water were injected into the box. By using this method, samples did not move in water during observation.Brightness of field in optical microscope with high magnification was kept by changing 30 W of halogen lamp into 50 W of halogen lamp. Halation occurring in observation of samples was eliminated by image processing.As a result of observing hydration process of cement minerals with defferent types of mixing water, it was clarified that speed of the system was fast enough to observe changes in morphology of calcium aluminate hydrates, while faster speed was needed in order to observe changes in morphology of calcium sulphoaluminate hydrates.

光学显微镜的缺点是只能聚焦有限的深度。为了科普这些缺点，本研究发展了使用光学显微镜的立体显示系统。也就是说，通过使用图像处理技术，从在稍微不同的深度上拍摄的多个图像中提取聚焦区域，然后使用这些聚焦区域合成一组立体图像。本研究旨在解决该系统在实际应用中存在的问题，降低图像处理速度，观察水泥矿物水化过程中的形态变化，通过引入本研究购买的图形工作站，图像提取和处理所需的时间可以减少到原来的三分之一。采用可浸于液体的物镜透镜，解决了物镜的防水和样品在水中的固定问题，样品的固定是在载玻片上用硅橡胶制成盒，然后将样品和水注入盒中。该方法观察时样品在水中不移动，将30 W卤素灯更换为50 W卤素灯，保持了高倍光学显微镜视野的亮度。通过图像处理消除了观察过程中出现的光晕现象，并通过对不同类型混合水的水泥矿物水化过程的观察，明确了系统的速度足以观察到铝酸钙水化产物形貌的变化，而要观察到硫铝酸钙水化产物形貌的变化则需要更快的速度。