权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Kindling as a neuronal plasticity (Effect of dentate nucleus lesion upon amygdaloid kindling in cats)

点燃作为神经元可塑性（齿状核损伤对猫杏仁核点燃的影响）

基本信息

批准号：
05454313
负责人：
TSURU Noriko
金额：
$ 3.26万
依托单位：
MIYAZAKI MEDICAL COLLEGE
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1993
资助国家：
日本
起止时间：
1993 至 1995
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05454313/
关键词：
Kindling Amygdala cerebellar dentate nucleus rat microdialysis cat glutamate GABA Kindling dentate nucleus cerebellum CAT glutamate kindling Dentate rucleus Cerebellum cat

项目摘要

Amygdaloid kindling is used to investigate whether the cerebellar dentate nucleus participate in the neuronal plasticity of the cerebrum in cats. In this study we examined the behavioral change, the occurrence of epileptiform events and duration of afterdischarge during amygdaloid kindling under control conditions and after a contralateral cerebellar dentate lesion in 11 cats. The first stimulation induced stage 2 in five animals out of six in lesioned group, but one of five in control group. The numbers of spikes on the primary site of the amygdala and hippocampus in the lesionde group increased at first, however on the later stage of kindling, those were decreased compared to the control gourp. The spikes on the secondary site of the hippocampus increased more in the lesioned group. Threfore cerebellar dentate lesion facillitated the secondary epileptogenesis. The seizure-related changes of extracellular hippocampal glutamate (Glu) concentration during the development of amygdaloid k … More indling were monitored. The extracellular Glu level enhanced after stimulus and returned rapidly to baseline. In partially kindled rats (stage 3), the extracellular Glu level during the first minutes post stimulus was 2.5-3.5 fold that of the baseline, while fully kindled rats exhibited about a 5-fold increase in the Glu level.Then, we simultaneously monitored the seizure-related changes in extracellular hippocampal glutamate (Glu) and gamma-aminobutyric acid (GABA) concentration in brain dialysates in order to clarify the role of Glu and GABA in the development of kindling. Extracellular GABA concentration gradually, reaching a plateau level at 15-20 min.and lasting for several hours after stimulus at each stage. The enhancement of GABA level was about 1.5 times that of the baseline in the partially kindled stage, and was about 2.5 times of the baseline in the fullly kindled stage. The progressive, transient and stimulus-induced enhancement of extracellular Glu levels combined with long-lasting elevation of extracellular GABA levels in the bilateral ventral hippocampi results in hypersynchrony and imbalance between the excitatory and inhibitory neuronal systems, causing excessive propagation of seizure activity, culminating in the secondary generalized seizure of amygdaloid kindling. Less

本实验采用猫杏仁核点燃模型，探讨小脑齿状核是否参与大脑神经元的可塑性。在这项研究中，我们研究了11只猫的行为变化，癫痫样事件的发生和持续时间的杏仁核点燃控制条件下，对侧小脑齿状核病变后。第一次刺激在损伤组的6只动物中的5只中诱导了第2阶段，而在对照组的5只中有1只。与对照组相比，损伤组杏仁核和海马的初级部位放电数先增加，到点燃后期，放电数减少。海马次级部位的棘波在损伤组增加更多。小脑齿状回损害易诱发继发性癫痫。杏仁核发育过程中海马细胞外谷氨酸浓度的变化 ...更多信息监测indling。刺激后细胞外Glu水平升高，并迅速恢复至基线水平。在部分点燃的大鼠（阶段3）中，刺激后第一分钟期间的细胞外Glu水平是基线的2.5-3.5倍，而完全点燃的大鼠表现出Glu水平增加约5倍。我们同时监测了海马细胞外谷氨酸（Glu）和γ-氨基丁酸（GABA）的变化，为了阐明Glu和GABA在点燃发展中的作用，需要测定脑透析液中的浓度。细胞外GABA浓度逐渐升高，在刺激后15-20 min达到平台水平，并持续数小时。GABA水平在部分点燃期约为基线的1.5倍，在完全点燃期约为基线的2.5倍。在双侧腹侧海马中，细胞外Glu水平的进行性、短暂性和刺激诱导的增强与细胞外GABA水平的长期升高相结合，导致兴奋性和抑制性神经元系统之间的超同步性和不平衡，引起癫痫发作活动的过度传播，最终导致杏仁核点燃的继发性全身性癫痫发作。少