Study of the IP_3 receptor family and its diverse roles in various physiological functions

IP_3受体家族及其在多种生理功能中的多种作用的研究

基本信息

  • 批准号:
    05455006
  • 负责人:
  • 金额:
    $ 4.74万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for General Scientific Research (B)
  • 财政年份:
    1993
  • 资助国家:
    日本
  • 起止时间:
    1993 至 1994
  • 项目状态:
    已结题

项目摘要

In this study, we have analyzed the structural and functional diversity of the IP_3 receptor family.1.We have cloned human type 1,2, and 3 receptor cDNAs of the IP_3R family, and have studied their structures, IP_3-binding activities, expression in various tissues and cell-lines, and chromosomal loci.2.We have localized the IP_3-binding site, calmodulin binding site and two N-linked glycosylation sites on the mouse type 1 IP_3 receptor. We have proposed a membrane-spanning model and a channel pore region.3.We have studied the expression of the type 1 receptor in various cell systems ; the up-regulation during the differentiation of a HL-60 cell line toward a neutrophilic cell lineage, the down-regulation in an SH-SY5Y neuroblastoma chronically stimulated with carbacol, and the reduction in cerebella of human spinocerebellar degeneration patients (olivopontocerebellar atrophy [OPCA] and hereditary cerebellar atrophy of Holmes type).4.We have developed transgenic mice carrying the fusion gene composed of the type 1 receptor promoter and beta-galactosidase gene, by which dynamic expression of the type 1 receptor can be easily visualized by assaying beta-galactosidase activity with chromogenic substrates.5.We have studied the expression of other molecules involved in calcium increase in neuronal cells, i.e., ryanodine receptors and voltage-dependent calcium channels, and have suggested the presence of complex calcium signalling systems in the nervous system.
本研究分析了IP_3受体家族的结构和功能多样性:1.克隆了人IP_3受体家族的1、2和3型受体cDNA,并对其结构、IP_3结合活性、在不同组织和细胞系中的表达以及染色体定位进行了研究; 2.定位了IP_3结合位点,小鼠1型IP_3受体上的两个N-糖基化位点和两个钙调蛋白结合位点。我们提出了一个跨膜模型和一个通道孔区。3.我们研究了1型受体在不同细胞系统中的表达;在HL-60细胞系向嗜酸性细胞谱系分化过程中的上调,在用卡巴可长期刺激的SH-SY 5 Y神经母细胞瘤中的下调,脊髓小脑变性患者小脑的缩小(橄榄体桥脑小脑萎缩[OPCA]和Holmes型遗传性小脑萎缩)4.我们已经开发了携带由1型受体启动子和β-半乳糖苷酶基因组成的融合基因的转基因小鼠,通过该方法,可以通过测定β-半乳糖苷酶与显色底物的活性5.我们研究了参与神经元细胞中钙增加的其他分子的表达,即,Ryanodine受体和电压依赖性钙通道,并已表明在神经系统中存在复杂的钙信号系统。

项目成果

期刊论文数量(152)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Wojcikiewicz,R.J.H.: "Muscarinic receptor activation down-regulates the type I inositol 1,4,5-trisphosphate receptor by accelerating its degradation" J.Biol.Chem.269. 7963-7969 (1994)
Wojcikiewicz,R.J.H.:“毒蕈碱受体激活通过加速其降解来下调 I 型肌醇 1,4,5-三磷酸受体”J.Biol.Chem.269。
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    0
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Ryo,Y.: "Immunohistochemical localization of metabotropic and ionotropic glutamate receptors in the mouse brain." Ann.New York Acad.Sci.707. 554-556 (1993)
Ryo,Y.:“小鼠大脑中代谢型和离子型谷氨酸受体的免疫组织化学定位。”
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    0
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Furuichi,T.: "Inositol 1,4,5-trisphosphate receptor-mediated Ca^<2+> signaling in the brain." J.Neurochem.(印刷中).
Furuichi, T.:“大脑中肌醇 1,4,5-三磷酸受体介导的 Ca^2+ 信号传导。”J. Neurochem。
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    0
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Aruga,J.: "Anovel zincfingerprotein,Zic,is involvedin neurogenesis,especially in the cell lineage of cerebellar granulecells." J.Neuochem.63:1880-1890,(1994).63. 1880-1890 (1994)
Aruga, J.:“一种新型锌指蛋白 Zic 参与神经发生,特别是小脑颗粒细胞的细胞谱系。”
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    0
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Yamamoto-Hino M: "Cloning and characterization of human type 2 and type 3 inositol 1,4,5-trisphosphate receptors" Receptors and Channels. (In press). (1994)
Yamamoto-Hino M:“人类 2 型和 3 型肌醇 1,4,5-三磷酸受体的克隆和表征”受体和通道。
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    0
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FURUICHI Teiichi其他文献

FURUICHI Teiichi的其他文献

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{{ truncateString('FURUICHI Teiichi', 18)}}的其他基金

Study on the molecular mechanisms underlying CAPS-mediated exocytosis of dense-core vesicles containing BDNF and catecholamine
CAPS介导的BDNF和儿茶酚胺致密核囊泡胞吐作用的分子机制研究
  • 批准号:
    23300137
  • 财政年份:
    2011
  • 资助金额:
    $ 4.74万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Study of Molecular Organization of Mouse Cerebellum
小鼠小脑分子组织的研究
  • 批准号:
    13308047
  • 财政年份:
    2001
  • 资助金额:
    $ 4.74万
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)
Study of the Structure-Function of IPィイD23ィエD2 Receptor/CaィイD12+ィエD1 Release Channel and IPィイD23ィエD2/CaィイD12+ィエD1 Signaling
IPD23D2受体/CaD12+D1释放通道结构功能及IPD23D2/CaD12+D1信号传导的研究
  • 批准号:
    10490007
  • 财政年份:
    1998
  • 资助金额:
    $ 4.74万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Study of the Molecular Structure and Function of IP_3 Receptor/Ca^<2+> Release Channel
IP_3受体/Ca^<2>释放通道的分子结构与功能研究
  • 批准号:
    08459009
  • 财政年份:
    1996
  • 资助金额:
    $ 4.74万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)

相似海外基金

Study of IP_3 receptor/Ca^<2+> signaling in neural plasticity and brain development and differentiation
IP_3受体/Ca^2信号在神经可塑性和脑发育分化中的研究
  • 批准号:
    20220007
  • 财政年份:
    2008
  • 资助金额:
    $ 4.74万
  • 项目类别:
    Grant-in-Aid for Scientific Research (S)
Analysis of the role of IP_3 receptor in higher brain function
IP_3受体在高级脑功能中的作用分析
  • 批准号:
    20500301
  • 财政年份:
    2008
  • 资助金额:
    $ 4.74万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Study for IP_3 - detecting system of IP_3 receptor
IP_3的研究——IP_3受体检测系统
  • 批准号:
    13357001
  • 财政年份:
    2001
  • 资助金额:
    $ 4.74万
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)
Role of IP_3 receptor/ Ca^<2+> signaling for synaptic plasticity and development and differentiation of brain
IP_3受体/Ca^2信号对突触可塑性和大脑发育分化的作用
  • 批准号:
    13308044
  • 财政年份:
    2001
  • 资助金额:
    $ 4.74万
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)
Role of IP_3 receptor/Ca^<2+> signaling in neural plasticity and brain development
IP_3受体/Ca^2信号在神经可塑性和大脑发育中的作用
  • 批准号:
    11308032
  • 财政年份:
    1999
  • 资助金额:
    $ 4.74万
  • 项目类别:
    Grant-in-Aid for Scientific Research (A).
Study of the Molecular Structure and Function of IP_3 Receptor/Ca^<2+> Release Channel
IP_3受体/Ca^<2>释放通道的分子结构与功能研究
  • 批准号:
    08459009
  • 财政年份:
    1996
  • 资助金额:
    $ 4.74万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Is 240kDa protein, a substrate of G-kinase, IP_3 receptor of smooth muscle?
240kDa 蛋白(G 激酶的底物)是平滑肌的 IP_3 受体吗?
  • 批准号:
    07670102
  • 财政年份:
    1995
  • 资助金额:
    $ 4.74万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Moleculan mechanism of IP_3 receptor Ca^<2+> channel and the role of the receptor in signal transduction and growth and development
IP_3受体Ca^2通道的分子机制及其在信号转导和生长发育中的作用
  • 批准号:
    02101001
  • 财政年份:
    1990
  • 资助金额:
    $ 4.74万
  • 项目类别:
    Grant-in-Aid for Specially Promoted Research
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