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Biopsychological study on learning deficits in chronic vitamin E-deficient rats

慢性维生素E缺乏大鼠学习障碍的生物心理学研究

基本信息

批准号：
05610055
负责人：
ICHITANI Yukio
金额：
$ 1.15万
依托单位：
University of Tsukuba
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1993
资助国家：
日本
起止时间：
1993 至 1994
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05610055/
关键词：
vitamin E learning memory acetylcholine rat 電撃感受性学習・記憶

项目摘要

We have already reported the possibility that chronic vitamin E deficiency causes learning impairment. In this study, we first investigated the biochemical changes of central nervous system mediating this learning impairment with special reference to the brain cholinergic neuron system, since this system has been shown related to learning and memory. Rats were fed vitamin E-deficient [VE (-)], -supplemented [VE (+)], or control food beginning after the age of 4 weeks until 24 months. At this time serum and brain vitamin E concentration was significantly different between 3 groups. Choline acetyltransferase and acetylcholinesterase activities measured as markers for brain cholinergic function were not affected by long-term vitamin E deficiency and supplementation in all brain regions measured. Thus results showed that chronic vitamin E deficiency did not lead to lower levels of activity in brain cholinergic enzymes.Next we investigated the histological changes in the peripheral nervous system and behavioral changes in response to noxious stimulus. Rats were fed 3 kinds of diet mentioned above and they were tested with flight responses to electrical foot shock at the age of 9 months. Rats fed VE (-) diet showed significantly lower sensitivity to the foot shock compared with VE (+) and control groups. According to a histological observation of the spinal dorsal root ganglia, the number of neurons in VE (-) group was not different from that in other 2 groups. However, the location of satellite cells surrounding these neurons tended to be disordered. Thus our results suggest learning impairment in passive avoidance response caused by chronic vitamin E deficiency may partly come from the lower shock sensitivity associated with peripheral nervous system dysfunction.

我们已经报道了慢性维生素E缺乏导致学习障碍的可能性。在这项研究中，我们首先研究了中枢神经系统介导的这种学习障碍的生化变化，特别是脑胆碱能神经元系统，因为该系统已被证明与学习和记忆有关。大鼠从4周龄开始至24个月龄，喂食维生素E缺乏[VE（-）]、维生素E补充[VE（+）]或对照食物。此时，血清和脑维生素E浓度在3组之间存在显著差异。胆碱乙酰转移酶和乙酰胆碱酯酶的活动作为脑胆碱能功能的标志物测量不受长期维生素E缺乏和补充在所有测量的大脑区域。因此，结果表明，慢性维生素E缺乏并没有导致脑胆碱能酶活性水平降低。接下来，我们研究了周围神经系统的组织学变化和对伤害性刺激的行为学变化。用上述3种饲料喂养大鼠，在9月龄时进行足电击飞行反应测试。与VE（+）组和对照组相比，VE（-）组大鼠对足电击的敏感性显著降低。脊髓背根神经节组织学观察，VE（-）组神经元数目与其他2组无明显差异。然而，这些神经元周围的卫星细胞的位置往往是无序的。因此，我们的研究结果表明，慢性维生素E缺乏引起的被动回避反应的学习障碍可能部分来自与周围神经系统功能障碍相关的休克敏感性降低。