New approach for an optical biopsy in melanoma diagnostics

光学活检诊断黑色素瘤的新方法

• 批准号: 432242402
• 负责人: Professor Dr. Steffen Emmert
• 金额: --
• 依托单位: Hannoversches Zentrum für Optische Technologien (HOT)
• 依托单位国家: 德国
• 项目类别: New Instrumentation for Research
• 资助国家: 德国
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/432242402?language=en
• 关键词: New; approach; optical; biopsy; melanoma

Malignant melanoma is the most dangerous type of skin cancer and has the highest mortality rate. Since the 1980s, the incidence rate has more than tripled. This project addresses an existing deficit in melanoma diagnostics. For a suspicious skin lesion it is often not clear whether it is malignant or benign. The tumor thickness, i.e. the depth of invasion of melanoma cells, is the decisive criterion apart from the dignity (malignant or benign) for assessing the tumor stage and the prognosis of the disease. It also determines the further treatment. According to the current gold standard, a reliable diagnosis of melanoma and the determination of the actual thickness are only possible by histopathology. Reliable, timely and non-invasive diagnostics are currently not possible, but would signify a clear improvement and initiate a paradigm shift in melanoma diagnostics.The overall goal of this project is the development of a new, integrative device that will enable the determination of both (i) the invasion depth and (ii) the dignity of suspected melanoma skin lesions preoperatively, for the first time, leading to reliable diagnosis. To obtain all relevant information, three optical modalities - optical coherence tomography (OCT), optoacoustics (OA) and Raman spectroscopy (RS) - will be combined for the first time in a multimodal device, the accessible skin parameters explored and their diagnostic capability evaluated. Also, the associated intelligent, au-tomated evaluation procedures will be established and the functionality validated in mouse and patient measurements in the clinic.The challenges of the project are (i) the realization of a fast (< 15 min) imaging system through combination of the three modalities, (ii) the reliable determination of the invasion depth of lesions using OCT and OA (up to 1.5 mm with OCT and several mm with OA) with similar resolution (<15 μm and <30 μm, resp.), (iii) the determination of lesion dignity by OCT (morphology) and RS (chemical composition) with high sensitivity (> 90%) and specificity (> 70%), and (iv) the implemen-tation of evaluation algorithms relying on generated data bases that will enable faithful and rapid lesion determination by multivariate data analysis, 3D segmentation, and deep learning algorithms.At the end of the proposed 3 year funding period, the suitability of the new device with regard to determination of invasion depth and dignity of melanoma will be demonstrated and validated. The multimodal system should ensure, for the first time, an optical biopsy in melanoma diagnosis with high sensitivity and specificity. In a potential second 2-year funding period, the suitability of the device shall be evaluated in a clinical pilot study. Also the potential with regard to identification of diagnostic criteria for other skin diseases, i.e. inflammatory skin diseases and different skin cancer types, as well as disease progression and melanoma therapy studies can be investigated.

恶性黑色素瘤是皮肤癌最危险的类型，死亡率最高。自1980年代以来，发病率的三倍多。该项目解决了黑色素瘤诊断中现有的赤字。对于可疑的皮肤病变，通常不清楚它是恶性还是良性。肿瘤厚度，即黑色素瘤细胞的侵袭深度，是决定性标准，除了评估肿瘤阶段和疾病预后的尊严（恶性或良性）外。它还决定了进一步的治疗方法。根据当前的黄金标准，仅通过组织病理学才有可能对黑色素瘤的可靠诊断和实际厚度的确定。 Reliable, timely and non-invasive diagnostics are currently not possible, but would signify a clear improvement and initiate a paradigm shift in melanoma diagnostics.The overall goal of this project is the development of a new, integrative device that will enable the determination of both (i) the invasion depth and (ii) the dignity of suspected melanoma skin lesions preoperatively, for the first time, leading to reliable diagnostics.为了获得所有相关信息，将首次合并三种光学模式 - 光学相干断层扫描（OCT），光顺序（OA）和拉曼光谱（RS） - 首次将在多模式设备中首次合并，即探索的可访问的皮肤参数及其诊断功能。同样，将建立相关的智能，自动化的评估程序，并在诊所中的鼠标和患者测量中验证的功能。该项目的挑战是（i）通过三种方式结合了快速（<15分钟）成像系统的快速（<15分钟）成像系统，（ii）使用oca和oca oca oca oca oca oca的可靠确定（ii）（ii）（ii）（ii）（ii）（ii）（ii）（ii）（ii）（ii）（ii）oc.5 mmmmmmmmmmmmmm（ 15μm和<30μm，分别为），（iii）通过OCT（形态学）和RS（化学组成）确定病变的尊严，具有高灵敏度（> 90％）和特异性（> 90％）和（> 70％），（> 70％），以及（iv）通过依赖于生成的数据基础的评估算法的实现，将其依赖于生成的数据进行分析，并将其依赖于生成的数据进行分析，并将其迅速分析，并将其快速分析，并将其快速分析，并将其依赖于生成的数据，并将其快速分析，并将其依赖于生成的数据，并将其快速分析，并将其依赖于生成的数据，并将其快速及深度学习算法。在拟议的3年筹资期结束时，将证明和验证新装置对确定黑色素瘤的入侵深度和尊严的适用性。多模式系统应首次确保具有高灵敏度和特异性的黑色素瘤诊断中的光学活检。在潜在的第二个2年资金期内，应在一项临床试点研究中评估该设备的适用性。还可以研究其他皮肤病的诊断标准，即炎症性皮肤病和不同的皮肤癌类型的潜力，以及疾病进展和黑色素瘤治疗研究。