Recovery and recycling of phosphorous from aquatic ecosystems by use of the salt-tolerant green algae Dunaliella and chitosan

利用耐盐绿藻杜氏藻和壳聚糖从水生生态系统中回收和再循环磷

• 批准号: 09650841
• 负责人: SUZUKI Takahiro
• 金额: $ 1.79万
• 依托单位: TOKYO INSTITUTE OF TECHNOLOGY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09650841/
• 关键词: micro-algae; Dunaliella; photosynthesis; polyphosphates; bio-concentration; fixation of P

A basic study on the development of a recovery and recycling process for phosphorous (P) from aquatic ecosystems by use of the salt-tolerant green algae Dunaliella and chitosan was performed. The effects of various combinations of environmental or operational factors on P content in the Dunaliella cells were examined, using laboratory cultures under controlled conditions. The factors examined were the culture temperature, light intensity, CO_2 concentration in flushing gas, P, NaCl, nitrogen and Ca^<2+> concentration in the culture. The experimental system of the factors and the measured responses of algal growth rate and P content was modeled and analyzed based on the response surface method. The model obtained revealed that the intracellular P content in D.parva was highly dependent on the P concentration in the pre-culture medium in the case of the P concentration in the culture was relatively low. It was also found that procaine hydrochloride was useful to increase the growth of the algal cells. In the next step, the P recovery in terms of physico-chemical treatments from the harvested algal cells was studied. The results showed the Ca(0H)_2 or Al_2(SO_4)_3 treatment for the polyphosphates in the algal cells was effective to precipitate phosphate salt. The biopolymers, natural chitin and chitosan, were not so effective at solid removal. Further study on the use of modified chitosan such as iron chitosan will be required.

一项关于通过使用耐盐绿藻Dunaliella和壳聚糖从水生生态系统中开发磷（P）开发的基础研究。在受控条件下，使用实验室培养物，检查了环境或操作因素的各种组合对Dunaliella细胞中P含量的影响。所检查的因素是培养气体的培养温度，光强度，CO_2浓度，P，NaCl，氮和Ca^<2+>浓度在培养物中。根据响应表面方法对因素和藻类生长速率和P含量的测量反应进行了实验系统。获得的模型表明，在培养物中P浓度的情况下，D.Parva中的细胞内P含量高度依赖于培养培养基中的P浓度。还发现盐酸普遍有助于增加藻类细胞的生长。在下一步中，研究了从收获的藻类细胞的物理化学处理方面恢复的P。结果表明，Ca（0H）_2或Al_2（SO_4）_3对藻类细胞中的多磷酸盐处理可有效沉淀磷酸盐盐。天然几丁质和壳聚糖的生物聚合物在固体去除方面并不那么有效。将需要进一步研究使用改良壳聚糖，例如铁壳壳。

项目成果

Takahiro Suzuki: "Influence of Environmental Factors on Phosphorous Content in the Marine Microalga Dunaliella" Toxicological and Environmental Chemistry. 64. 173-181 (1997)

Takahiro Suzuki：“环境因素对海洋微藻杜氏藻中磷含量的影响”毒理学和环境化学。

Takahiro Suzuki: "Influence of Environmental Factors on Fhosphorous Content in the Marine Microalga Dunaliella" Toxicological and Environmental Chemistry. 64. 173-181 (1997)

Takahiro Suzuki：“环境因素对海洋微藻杜氏藻中磷含量的影响”毒理学和环境化学。

Takahiro Suzuki: "Effect of Procaine Concentration on the Growth of Some Species of Microalgae and Cyanobacteria" Journal of Pharmacy and pharmacology. 50(Suppl.). 133-133 (1998)

Takahiro Suzuki：“普鲁卡因浓度对某些微藻和蓝藻物种生长的影响”药学和药理学杂志。

Takahiro Suzuki: "Effect of Procaine Concentration on the Growth of Some Species of Microalgae and Cyanobacteria" Journal of Pharmacy and Pharmacology. 50(Suppl.). 133 (1998)

Takahiro Suzuki：“普鲁卡因浓度对某些微藻和蓝细菌生长的影响”《药学与药理学杂志》。

Takahiro Suzuki: "Intluence of Environmental Factors on Phosphorous Content in the Marine Microalga Dunaliella" Toxicological and Environmental Chemistry. 64. 173-181 (1997)

Takahiro Suzuki：“环境因素对海洋微藻杜氏藻中磷含量的影响”毒理学和环境化学。