Evolution and taxonomical stydy on the genus Oryzae by mitochondrial differentiation (1994)

通过线粒体分化对稻属进行进化和分类学研究（1994）

• 批准号: 05660003
• 负责人: ABE Toshinori
• 金额: $ 1.41万
• 依托单位: Yamagata University, Faculty of Agriculture
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05660003/
• 关键词: O.sativa; Oryzae; Mitochondrial genome; RFLP; Dendrogram; Evolution; O.sativa; ミトコンドリア; イネ属類縁関係; サザンブロッティング; イネ属の進化

Restriction fragment length polymorphisms (RFLP) of mitochondrial DNA were invstigated using 10 rice varieties including japonica, indica and javanica subspesies. RFLPs of cultivated rice were distinguished using the restriction endonuclease, Xba I and non-radioactive mitochondrial gene probs. When the probes, atp6, coxI and rrn18 were used, the differences between subspecies i.e.japonica and indica were detected. Delation (0.7kb) in indica rice varieties was found when rrn18 probe was hybridized with mitochondrial DNA digested by XbaI.RFLPs of genus Oryzae (13 speciies and 22 lines) were revealed by 8 mitochondrial DNA probes. The relationships between RFLP patterns of the mitochondrial DNA were studied by a cluster analysis of genetic distance based on ratio of shared bands. O.sativa complex was included in one cluster, and indica varieties were closed to O.glaberrima. O.officinalis complex showed a remarkably higher amount of variation. In BB and BBCC genomes, O.punctata (BBCC) and O.minuta (BBCC) were separated. O.punctata (BB) belonged to either BBCC genome species i. e. O.punctata (BBCC) and O.minuta, (BBCC). These result indicated that the BBCC genome species, O.punctata (BBCC) and O.minuta (BBCC) were raised from BB genome of O.punctata. O.officinalis (CC), O.eichingeri (CC), O.latifolia (CCDD) and O.alta (CCDD) were closely related each other. O.australiensis (EE) and O.brachyanth (FF) were far distant from cultivated species of O.sativa.

使用包括粳稻、籼稻和爪哇亚种在内的 10 个水稻品种，研究了线粒体 DNA 的限制性片段长度多态性 (RFLP)。使用限制性内切酶、Xba I 和非放射性线粒体基因探针来区分栽培稻的 RFLP。当使用atp6、coxI和rrn18探针时，检测到粳稻和籼稻亚种之间的差异。 rrn18探针与XbaI酶切的线粒体DNA杂交，发现籼稻品种存在延迟（0.7kb）。8个线粒体DNA探针揭示了稻属（13种22系）的RFLP。通过基于共享带比率的遗传距离聚类分析来研究线粒体 DNA 的 RFLP 模式之间的关系。 O. sativa 复合体包含在一个簇中，籼稻品种与 O. glaberrima 接近。 O.officinalis 复合体表现出明显更高的变异量。在 BB 和 BBCC 基因组中，O.punctata (BBCC) 和 O.minuta (BBCC) 是分开的。 O.punctata (BB) 属于 BBCC 基因组物种 i。 e. O.punctata (BBCC) 和 O.minuta, (BBCC)。这些结果表明BBCC基因组种O.punctata (BBCC)和O.minuta (BBCC)是从O.punctata的BB基因组中培育出来的。 O.officinalis (CC)、O.eichingeri (CC)、O.latifolia (CCDD) 和 O.alta (CCDD) 彼此关系密切。 O.australiensis (EE) 和 O.brachyanth (FF) 与栽培稻种距离较远。

项目成果

阿部利徳: "ミトコンドリアDNAからみたイネ属植物の系統分化" IGEシリーズ 植物の系統分化及び遺伝変異の誘導と解析. 18. 39-49 (1994)

Toshinori Abe：“从线粒体DNA角度观察禾本科植物的系统发育分化” IGE系列植物谱系分化和遗传变异的诱导和分析。 18. 39-49 (1994)

阿部利徳: "イネカルスおよび再分化植物におけるオルガネラDNAのRFLP V.種子由来カルスおよび葯由来カルスにおけるmtDNA変異の相違" 育種学雑誌(印刷中). 44巻(別1). (1994)

Toshinori Abe：“水稻愈伤组织和再生植物中细胞器 DNA 的 RFLP V。种子来源的愈伤组织和花药来源的愈伤组织中 mtDNA 突变的差异”《育种杂志》第 44 卷（第 1 部分）（1994 年）。

ABE T., et al.: "RFLP analysis of rice mitochondrial DNA.I.Ecotypical and varietal variation in rice mitochondrial DNA." Japan.J.Breed.42 (Ext.1). 228-229 (1992)

ABE T. 等人：“水稻线粒体 DNA 的 RFLP 分析。I.水稻线粒体 DNA 的生态典型和品种变异。”

ABE T.: "Taxonomical differentiation of genus Oryzae in conderned with mitochondrial DNA." IGE in Institute of Genetic Ecology in Tohoku Univ.18. 39-49 (1994)

ABE T.：“米属的分类学分化与线粒体 DNA 的关系。”

阿部利徳: "イネミトコンドリアDNAのRFLP分析 II.種間差異" 育種学雑誌. 42巻(別2). 244-245 (1992)

Toshinori Abe：“水稻线粒体 DNA 的 RFLP 分析 II。种间差异”《育种科学杂志》第 42 卷（第 2 部分）（1992 年）。