Studies on the signal transduction network in adaptive responses in microorganisms.

微生物适应性反应信号转导网络的研究。

• 批准号: 05660097
• 负责人: KATO Junichi
• 金额: $ 1.41万
• 依托单位: HIROSHIMA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05660097/
• 关键词: Adaptive responses; Signal transduction; Information processing; Microorganism; Chemotaxis; Pho regulon; Phoレギュロン; シグナル伝達系

The final goal of our research is to understand the signal transduction networks in a bacterial cell.We have focused on the signal transductions in the adaptation response system for phosphate starvation in Pseudomonas aeruginosa for two years and performedthe genetic analyzes of the Pi taxis and the phosphate regulon.The summary of research results is as follows :1.An constitutive alkaline phosphatase (AP) mutant, designated APC1, showed a constitutive phosphate (Pi) taxis.The result suggests the existence of a common negative regulator for the Pi regulon and the Pi taxis.1.A chromosomal phoB mutant still showed the inducible Pi taxis, indicating the Pi taxis is not regulated by the phoB gene unlike the Pi regulon.2.We cloned a 27-kb DNA fragment that complements the mutation of APC1.Genetic analyzes of the fragment revealed the negative regulator is the Pi specific transport channel complex.4.A 20-kb fragment was obtained by shot-gun cloning usibg a chemotaxis deficient mutant as a host strain.Two chemotaxis genes, cheY and cheZ were found on the fragment by genetic analyzes.

本研究的最终目的是了解细菌细胞中的信号转导网络。我们对铜绿假单胞菌磷饥饿适应反应系统中的信号转导进行了两年的研究，并对PI趋向性和磷酸盐调节进行了遗传分析。研究结果如下：1.碱性磷酸酶(AP)的结构性突变体APC1表现出结构性的PI趋向性。结果表明，存在一个共同的PI调节子和PI趋向性的负调控因子。1.染色体上的Phob突变体仍然显示出可诱导的PI趋性。2.克隆了一个27kb的DNA片段，该片段与APC1基因的突变互补。遗传分析表明，该负调控因子是PI特异的转运通道复合体。4.以趋化性缺失突变体为宿主菌株，通过枪击克隆获得了一个20kb的片段，遗传分析发现该片段上有两个趋化性基因Chey和chez。

项目成果

Junichi KATO: "Cloning and characterization of a Pseudomonas aeruginosa gene involved in tne negative regulation of phosphate taxis" J.Bacteriol.176. 5874-5877 (1994)

Junichi KATO：“参与磷酸盐负调控的铜绿假单胞菌基因的克隆和表征”J.Bacteriol.176。

Agus MASDUKI: "Isolation and characterization of chemotaxis mutants and genes of Pseudomonas aeruginosa" J.Bacteriol.177. 948-952 (1995)

Agus MASDUKI：“铜绿假单胞菌趋化突变体和基因的分离和表征”J.Bacteriol.177。

HARDOYO,Shunsuke KAJIWARA,Junichi KATO,and Hisao OHTAKE: "Bacterial motility as a potential tool for rapid toxicity assays" Biotechnique. 7(7). 457-462 (1993)

HARDOYO、Shunsuke KAJIWARA、Junichi KATO 和 Hisao OHTAKE：“细菌运动作为快速毒性测定的潜在工具”生物技术。

Junichi KATO,and Hisao OHTAKE: "Signal transduction pathways in the assimilation system for phosphate in bacteria" Bulletin of Japanese Society of Microbial Ecology. 8(3). 183-189 (1993)

加藤纯一 (Junichi KATO) 和大竹久男 (Hisao OHTAKE)：“细菌中磷酸盐同化系统中的信号转导途径”日本微生物生态学会通报。

Agus Masduki: "Isolation and characterization of chemotaxis mutants and genes of Pseudomonas aeruginosa." J.Bacteriol.177. 948-952 (1995)

Agus Masduki：“铜绿假单胞菌趋化突变体和基因的分离和表征。”