Studies on bilirubin metabolism and in vivo lipid peroxidation of fish.

1.比较了养殖和野生黄鱼体内肝脏和血浆中的脂质过氧化水平和抗氧化活性。结果表明，养殖鱼类可能比野生鱼类遭受更多的氧化应激，在黄尾鱼的饲料中补充α-生育酚和抗坏血酸是必要的，以防止体内脂质过氧化的进展，从而导致许多疾病。感染红细胞包涵体综合征(EIBS)病毒的鲑鱼(Oncorhynchus Kiutch)出现高胆红素血症。病鱼体内胆红素分泌紊乱是感染Eibs病毒的鲑鱼高胆红素血症的原因之一。胆绿素在体外是重氮化的负性物质，在血浆或胆汁中成为重氮化阳性物质。因此，不能用重氮法分析含有胆绿素的鱼血浆中的胆红素水平。用…饲喂黄尾五斑潜蝇更多关于不同α-生育酚(α-Toc)水平的挤出机日粮(EP)。α-ToC含量与2-硫代巴比妥酸活性物质(TBARS)值呈负相关。虽然每种饲料的抗坏血酸水平相同，但高水平α-ToC饲料的肝脏水平高于低水平α-ToC饲料的肝脏水平。注射细菌的血浆中胆红素含量与TBARS值呈线性相关，这可能是引起黄疸的原因之一。然而，胆红素含量与细菌数量之间不存在线性相关。用抗胆红素单抗24G7建立的酶联免疫吸附试验(EL ISA)检测了7种重氮反应阴性的正常人尿液中的胆红素代谢物。分离得到7种代谢物中的2种，其化学结构经快原子轰击-质谱仪(FAB-MS)和核磁共振氢谱确证，两种代谢物分别为1，14，15，17-tetrahydro-2，7，13-trimethy1-1，14-dioxo3-vinyl-16H-tripyrrin-8，12-dipropionic酸和1，14，15，17-tetrahydro-3，7，13-trimethy11，14-dioxo-2-vinyl-16H-tripyrrin-8，12-dipropionic酸。较少

1. In vivo lipid peroxidation levels and antioxidant activities in the liver and plasma of cultured yellowtail Seriola quinqueradiata were compared with those of wild fish. Results obtaianed suggest that cultured fish may suffer from more oxidative stress than wild fish and that alpha-tocopherol and ascorbic acid supplements are necessary in the diets of yellowtail to prevent the progress of in vivo lipid peroxidation which brings about many diseases.2. Hyperbilirubinemia occurred in coho salmon, Oncorhynchus kisutch, infected with erythrocytic inclusion body syndrome (EIBS) virus. Secretory disorder of bilirubin occurred in the diseased fish was one of the causes of hyperbilirubinemia observed in coho salmon infected with EIBS virus.3. Biliverdin being a diazo negative substance in vitro become a diazo positive sustance in plasma or bile. Therefore bilirubin level in fish plasma in which biliverdin exists must not analyzed by diazo method.4. Yellow tail Seriola quinqueradiata were fed … More on extruder diets (EP) with different alpha-tocopherol (alpha-Toc) level. Reverse relation was observed between alpha-Toc contents and 2- thiobarbituric acid reactive substances (TBARS) values. Although ascorbic acid level of each diet was the same, the levels in the liver fed on diet containing higer level of alpha-Toc was higer than those containing lower level of alpha-Toc.5. Linear correlation was observed between TBARS values and bilirubin content in the plasma injected with bacteria which is thought to be one of the cause of jaundice. However, no linear correlation was observed between bilirubin contents and bacteria number.6. Seven bilirubin metabolites negative to the diazo reaction were identified in the urine of healthy persons by enzyme-linked immunosorbent assay (ELISA) using the anti-bilirubin monoclonal antibody 24G7. Two of the seven metabolites were isolated and their chemical structures were determined using fast-atom bombardment-mass spectroscopy (FAB-MS) and 1H-NMR.The two metabolites are 1,14,15,17-tetrahydro-2,7,13-trimethy1-1,14-dioxo3-vinyl-16H-tripyrrin-8,12-dipropionic acid and 1,14,15,17-tetrahydro-3,7,13-trimethy11,14-dioxo-2-vinyl-16H-tripyrrin-8,12-dipropionic acid. Less