Studies on bilirubin metabolism and in vivo lipid peroxidation of fish.

1.比较了人工养殖和野生黄尾鱼肝脏和血浆的脂质过氧化水平和抗氧化活性。结果表明，养殖鱼可能比野生鱼遭受更多的氧化应激，在黄尾鱼饲料中添加α-生育酚和抗坏血酸是必要的，以防止体内脂质过氧化的进展，从而导致许多疾病.红细胞包涵体综合征（EIBS）病毒感染银鲑后，出现高胆红素血症。感染EIBS病毒的银鲑出现胆红素分泌障碍是引起高胆红素血症的原因之一.胆绿素在体外为重氮阴性物质，在血浆或胆汁中为重氮阳性物质。因此，在含有胆绿素的鱼血浆中，胆红素含量不应采用重氮法测定.饲喂黄尾五斑瓢虫 关于我们 对不同α-生育酚（alpha-Toc）水平的挤压机日粮（EP）的影响。α-Toc含量与2-硫代巴比妥酸反应物质（TBARS）值呈负相关。虽然两种饲料的抗坏血酸水平相同，但高α-Toc水平饲料的肝脏中抗坏血酸水平高于低α-Toc水平饲料。TBARS值与注入细菌的血浆中胆红素含量之间观察到线性相关性，这被认为是黄疸的原因之一。胆红素含量与细菌数无线性相关关系.采用抗胆红素单克隆抗体24 G7，通过酶联免疫吸附试验（ELISA）在健康人尿液中鉴定出7种重氮反应阴性的胆红素代谢产物。从7种代谢产物中分离出2种，并使用快原子轰击质谱（FAB-MS）和1H-NMR确定了它们的化学结构。这2种代谢产物为1，14，15，17-四氢-2，7，13-三甲基-1，14-二氧代-3-乙烯基-16H-三吡咯-8，12-二丙酸和1，14，15，17-四氢-3，7，13-三甲基11，14-二氧代-2-乙烯基-16H-三吡咯啉-8，12-二丙酸。少

1. In vivo lipid peroxidation levels and antioxidant activities in the liver and plasma of cultured yellowtail Seriola quinqueradiata were compared with those of wild fish. Results obtaianed suggest that cultured fish may suffer from more oxidative stress than wild fish and that alpha-tocopherol and ascorbic acid supplements are necessary in the diets of yellowtail to prevent the progress of in vivo lipid peroxidation which brings about many diseases.2. Hyperbilirubinemia occurred in coho salmon, Oncorhynchus kisutch, infected with erythrocytic inclusion body syndrome (EIBS) virus. Secretory disorder of bilirubin occurred in the diseased fish was one of the causes of hyperbilirubinemia observed in coho salmon infected with EIBS virus.3. Biliverdin being a diazo negative substance in vitro become a diazo positive sustance in plasma or bile. Therefore bilirubin level in fish plasma in which biliverdin exists must not analyzed by diazo method.4. Yellow tail Seriola quinqueradiata were fed … More on extruder diets (EP) with different alpha-tocopherol (alpha-Toc) level. Reverse relation was observed between alpha-Toc contents and 2- thiobarbituric acid reactive substances (TBARS) values. Although ascorbic acid level of each diet was the same, the levels in the liver fed on diet containing higer level of alpha-Toc was higer than those containing lower level of alpha-Toc.5. Linear correlation was observed between TBARS values and bilirubin content in the plasma injected with bacteria which is thought to be one of the cause of jaundice. However, no linear correlation was observed between bilirubin contents and bacteria number.6. Seven bilirubin metabolites negative to the diazo reaction were identified in the urine of healthy persons by enzyme-linked immunosorbent assay (ELISA) using the anti-bilirubin monoclonal antibody 24G7. Two of the seven metabolites were isolated and their chemical structures were determined using fast-atom bombardment-mass spectroscopy (FAB-MS) and 1H-NMR.The two metabolites are 1,14,15,17-tetrahydro-2,7,13-trimethy1-1,14-dioxo3-vinyl-16H-tripyrrin-8,12-dipropionic acid and 1,14,15,17-tetrahydro-3,7,13-trimethy11,14-dioxo-2-vinyl-16H-tripyrrin-8,12-dipropionic acid. Less